Code | CSB-PA00029A0Rb |
Size |
US$299Purchase it in Cusabio online store (only available for customers from the US) |
Image |
|
The Latest Promotion | ![]() |
Have Questions? | Leave a Message or Start an on-line Chat |
Full Product Name | Rabbit anti-Homo sapiens (Human) GAPDH Polyclonal antibody | ||||||||
Uniprot No. | P04406 | ||||||||
Target Names | GAPDH | ||||||||
Alternative Names | 38 kDa BFA-dependent ADP-ribosylation substrate antibody; aging associated gene 9 protein antibody; Aging-associated gene 9 protein antibody; BARS-38 antibody; cb609 antibody; EC 1.2.1.12 antibody; Epididymis secretory sperm binding protein Li 162eP antibody; G3P_HUMAN antibody; G3PD antibody; G3PDH antibody; GAPD antibody; GAPDH antibody; Glyceraldehyde 3 phosphate dehydrogenase antibody; glyceraldehyde 3-PDH antibody; Glyceraldehyde-3-phosphate dehydrogenase antibody; HEL-S-162eP antibody; KNC-NDS6 antibody; MGC102544 antibody; MGC102546 antibody; MGC103190 antibody; MGC103191 antibody; MGC105239 antibody; MGC127711 antibody; MGC88685 antibody; OCAS; p38 component antibody; OCT1 coactivator in S phase; 38-KD component antibody; peptidyl cysteine S nitrosylase GAPDH antibody; Peptidyl-cysteine S-nitrosylase GAPDH antibody; wu:fb33a10 antibody | ||||||||
Raised in | Rabbit | ||||||||
Species Reactivity | Human, Mouse | ||||||||
Immunogen | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase protein (2-335AA) | ||||||||
Immunogen Species | Homo sapiens (Human) | ||||||||
Conjugate | Non-conjugated The GAPDH Antibody (Product code: CSB-PA00029A0Rb) is Non-conjugated. For GAPDH Antibody with conjugates, please check the following table. |
||||||||
Clonality | Polyclonal | ||||||||
Isotype | IgG | ||||||||
Purification Method | >95%, Protein G purified | ||||||||
Concentration | It differs from different batches. Please contact us to confirm it. | ||||||||
Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
||||||||
Form | Liquid | ||||||||
Tested Applications | ELISA, WB, IHC, IF | ||||||||
Recommended Dilution |
|
||||||||
Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol |
||||||||
Troubleshooting and FAQs | Antibody FAQs | ||||||||
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||||
Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Applications : WB
Sample type: Mouse RAW264.7 cells
Review: RAW264.7 cells were transformed with plasmids for RNA interference shRNA (Cont and ATF3i). After 24 h, carnosol was added to the culture medium, and ATF3 protein levels were examined by western blot analyses. The panel shows a representative set of genes analysed in duplicate. Carnosol only (third lane) resulted in the upregulation ATF3 protein.
By Anonymous
Applications : Western blot analysis
Sample type: cell
Review: Western blots and quantification of p21 and p16 protein levels normalized to GAPDH using protein extracts from the skin.
By Anonymous
Function |
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
|
Gene References into Functions |
|
Subcellular Location | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. |
Protein Families | Glyceraldehyde-3-phosphate dehydrogenase family |
Database Links |
HGNC: 4141 OMIM: 138400 KEGG: hsa:2597 STRING: 9606.ENSP00000229239 UniGene: Hs.544577 |