Code | CSB-RA009232A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:3000-1:10000 |
The coding sequence for the GAPDH monoclonal antibody isolated by immunizing animals with the human GAPDH synthesized peptide was cloned into the plasmids and then transfected into cell lines for in vitro expression. The product underwent affinity-chromatography-mediated purification to get the GAPDH recombinant monoclonal antibody. This GAPDH antibody is a rabbit IgG. It is suitable for the ELISA and WB to detect GAPDH protein from human and mouse samples.
GAPDH is a glycolytic enzyme that catalyzes the conversion of glyceraldehyde 3-phosphate to 1,3-diphosphoglycerate. Apart from its role as a glycolytic enzyme, GAPDH is a pleiotropic functional protein involved in the regulation of gene expression, DNA repair, and replication, neurodegeneration, pathogenesis, virulence in bacteria, tubular bundling, protein-protein interactions, RNA export, as well as apoptosis, and autophagy.
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