PTK2 Antibody

Datasheet
Code CSB-PA018993GA01HU
Size US$685
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Product Details

Uniprot No. Q05397
Target Names PTK2
Alternative Names FADK 1 antibody; FADK antibody; FAK related non kinase polypeptide antibody; FAK1 antibody; FAK1_HUMAN antibody; Focal adhesion kinase 1 antibody; Focal adhesion Kinase antibody; Focal adhesion kinase isoform FAK Del33 antibody; Focal adhesion kinase related nonkinase antibody; FRNK antibody; p125FAK antibody; pp125FAK antibody; PPP1R71 antibody; Protein phosphatase 1 regulatory subunit 71 antibody; Protein tyrosine kinase 2 antibody; Protein-tyrosine kinase 2 antibody; Ptk2 antibody; PTK2 protein tyrosine kinase 2 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Human FAK
Immunogen Species Homo sapiens (Human)
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
Tested Applications ELISA,WB,IHC
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
Gene References into Functions
  1. LFA-1 cross-linking recruits and activates FAK1 and PYK2 to phosphorylate LAT selectively on a single Y-171 site that binds to the GRB2-SKAP1 complex and limits dwell times of T-cells with dendritic cells PMID: 28699640
  2. Results identified FAK mRNA as a direct target of miR-433. Its activation inhibits the effect of microRNA433 on the growth of cervical cancer cells. PMID: 30272334
  3. This study shows that Leu33Pro polymorphism of integrin beta 3 modulates platelet Src pY418 and focal adhesion kinase pY397 phosphorylation in response to abnormally high shear stress. Whereas physiological shear stress does not affect platelet signaling, abnormally high-shear stress considerably elevates Src and FAK phosphorylation in both Pro33 and Leu33 platelets. PMID: 29965811
  4. High FAK expression is associated with gastric cancer. PMID: 30106432
  5. These results indicate that PCTK3 controls actin cytoskeleton dynamics by negatively regulating the FAK/Rho signaling pathway. PMID: 28361970
  6. FAK is required for adipocyte survival and maintenance of insulin sensitivity, particularly in the context of adipose tissue expansion as a result of caloric excess. PMID: 28165007
  7. Data suggest that TYRO3-mediated phosphorylation of ACTN4 is involved in invasiveness of melanoma cells; TYRO3-mediated phosphorylation of ACTN4 requires FAK activation at tyrosine 397. (TYRO3 = TYRO3 protein tyrosine kinase; ACTN4 = actinin alpha 4; FAK = focal adhesion kinase isoform FAK1) PMID: 29274473
  8. FAK controls invasiveness of tumor cells by regulating focal adhesion-mediated motility. PMID: 29133485
  9. FAK controls the nuclear translocation and activation of YAP in response to mechanical activation and submit that the YAP-dependent process of durotaxis requires a cell with an asymmetric distribution of active and inactive FAK molecules. PMID: 29070586
  10. Results show thatproto-Oncogene Protein ets-1 (ETS1) drives ovarian cancer (OC) metastasis phenotypes through its transcriptional target PTK2 (focal adhesion kinase FAK). PMID: 29174800
  11. Methylmercury chloride negatively affects the activation of Src, Rac1 and Cdc42, all of which are critical proteins for the regulation of cell movement. PMID: 29197552
  12. This study demonstrated that the Cas scaffolding protein family member 4 and protein tyrosine kinase 2 proteins and their significant role in the activation of downstream signaling pathways in Alzheimer's disease. PMID: 29789968
  13. Calpain small subunit 1 (Capn4) overexpression increased the protein level of cleaved talin and and activated the focal adhesion kinase (FAK)/AKT/MAPK signaling in 786-O cells, while Capn4 silencing decreased the protein level of cleaved talin in Caki-1 cells. PMID: 29648579
  14. mitochondria are present at the leading edge of migrating cells, SIRT3 expression is down-regulated during migration, resulting in elevated ROS levels. This SIRT3-mediated control of ROS represses Src oxidation and attenuates focal adhesion kinase (FAK) activation. PMID: 29915029
  15. These results demonstrated that the inhibition of FAK promoted cell detachment by decreasing the expression of focal adhesions components (talin and paxillin), and inhibiting cell motility by reducing the levels of Rho GTPases (Rac1, Cdc42 and RhoA). PMID: 29484384
  16. The results showed that in cervical cancer cells Rac1 activation by hypoxia could stimulate invasion and migration, and this process was mediated by integrin a5b3-facilitated FAK and PI3K phosphorylation. PMID: 29358562
  17. MUC4/X facilitated pancreatic cancer (PC) tumorigenesis via integrin-beta1/FAK/ERK signaling pathway. Overall, these findings revealed the novel role of MUC4/X in promoting and sustaining the oncogenic features of PC. PMID: 29777904
  18. The addition of LCS to capecitabine treatment led to an increase in the proteolysis of the FAK signaling cascade components. PMID: 30061234
  19. MPAP suppressed cancer cell proliferation and the phosphorylation of FAK1. Combined treatment with MPAP and irradiation (IR) showed enhanced suppression of cancer cell proliferation in wild-type p53 cells and more intense suppression in p53-null cells PMID: 29048635
  20. Optogenetic control of FAK signaling has been described. PMID: 29074139
  21. results suggest that W2 suppresses cancer cell migration and invasion by inhibiting FAK/STAT3 signaling and STAT3 translocation to the nucleus in monomorphic malignant human glioma cells. PMID: 28498494
  22. these results suggest that Ascochlorin inhibits cell migration and invasion by blocking FAK and JAK/STAT signaling, resulting in reduced MMP-2 activity. PMID: 28569433
  23. High levels of phosphorylated tyrosine-397 FAK in the nucleus of patient-derived melanoma tissues. PMID: 28348210
  24. The RNA-editing enzyme ADAR promotes lung adenocarcinoma migration and invasion by stabilizing FAK. PMID: 28928239
  25. The ectopic overexpression of miR-379 inhibited cell migration, invasion and EMT progress, while downregulated miR-379 reversed the effect. In addition, miR-379 regulated the focal adhesion kinase (FAK) by directly binding to its 3'-UTR, resulting in suppression of AKT signaling. In clinical samples of gastric cancer (GC), miR-379 inversely correlated with FAK, which was upregulated in GC. PMID: 28713929
  26. Building upon previous work suggesting that FAK-Akt1 binding is mediated by the FAK F1 lobe, we demonstrated that independently expressing the F1 domain in human Caco-2 or murine CT-26 colon cancer cells by transient or stable inducible plasmid expression respectively prevents the stimulation of cancer cell adhesion by increased extracellular pressure. PMID: 28820394
  27. functional activation of FAK1 in metastases and provide preclinical rationale for targeting this kinase in the setting of advanced ccRCC PMID: 28418903
  28. this study shows that simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar PMID: 27181267
  29. Silencing of p130Cas and inhibition of FAK activity both strongly reduced imatinib and nilotinib stimulated invasion. PMID: 27293031
  30. IP6K1 physiologically regulates neuronal migration by binding to alpha-actinin and influencing phosphorylation of both FAK and alpha-actinin through its product 5-diphosphoinositol pentakisphosphate. PMID: 28154132
  31. These data indicate that Ang II-AT2R regulates human bone marrow MSC migration by signaling through the FAK and RhoA/Cdc42 pathways. PMID: 28697804
  32. upregulated FAK expression correlates with poor prognosis and tumor dissemination in surgically treated patients with hypopharyngeal cancer PMID: 27061113
  33. These findings suggest that the integrin beta4-FAK/Src signaling axis may play a crucial role in clonorchiasis-associated cholangiocarcinoma metastasis during tumor progression. PMID: 28286026
  34. whereas Src activation under shear stress is dominantly ligand-dependent, FAK signaling seems to be mostly shear induced. PMID: 27467982
  35. The miR-7 can inhibit the activation of ERK/MAPK signaling pathway by down-regulating FAK expression, thereby suppressing the proliferation, migration and invasion of NSCLC cells. The miR-7 and its target gene FAK may be novel targets for the diagnosis and treatment of NSCLC. PMID: 27764812
  36. Thrombomodulin (TM) promotes angiogenesis by enhancing cell adhesion, migration, and FAK activation through interaction with fibronectin. PMID: 27602495
  37. FAK activation may facilitate tumour initiation by causing resistance to apoptosis. PMID: 27611942
  38. Among a group of tumor cells, there is correlation between activation of the MRTF-dependent transcription and activated FAK-dependent regulation of cell migration. PMID: 27708220
  39. Our study suggests that FOXM1 transcription factor regulates Integrin b1 gene expression and that FOXM1/ Integrin-b1/FAK axis may play an important role in the progression of Triple-negative breast cancer PMID: 28361350
  40. It has been demonstrated that FAK depletion reduces hepatocellular carcinoma cell growth by affecting cancer-promoting genes including the pro-oncogene EZH2. PMID: 28338656
  41. High FAK expression is associated with breast cancer cell invasion, transendothelial migration and metastasis. PMID: 26993780
  42. Study provides evidence that PTK2 expression is regulated by KCNMA1 in gastric tumorigenesis. PMID: 28231797
  43. HER2 reduces the radiosensitivity of breast cancer by activating Fak in vitro and in vivo. PMID: 27286256
  44. The interaction between FAK and tetraspan proteins in physiological and pathological conditions is reviewed. PMID: 27279237
  45. BKCa has a role in promoting growth and metastasis of prostate cancer through facilitating the coupling between alphavbeta3 integrin and FAK PMID: 27233075
  46. Proteomic analysis identified PTK2/FAK overexpression is a biomarker of radioresistance in locally advanced HNSCC, and PTK2/FAK inhibition radiosensitized HNSCC cells. PMID: 27036135
  47. FAK-Src-Paxillin system is a marker of unfavorable prognosis for human Neuroblastoma patients but also a promising therapeutic target. PMID: 29040002
  48. IGF-II siRNA inactivates the FAK/PI3K/Akt signaling pathway, and further reduces cell proliferation, N-ras and C-myc levels in SMMC-7721 cells. PMID: 27768959
  49. The purpose of this study was to determine the maximum tolerated dose (MTD), safety, pharmacokinetics (PK), and pharmacodynamics (PD) of the FAK inhibitor, GSK2256098, in cancer patients.GSK2256098 has an acceptable safety profile, has evidence of target engagement at doses at or below the MTD, and has clinical activity in patients with mesothelioma, particularly those with merlin loss PMID: 27733373
  50. Studies suggest that signaling pathways downstream of activated FAK including paxillin will be important to study in the context of FAK inhibition and other therapeutics to identify novel biomarkers. PMID: 26980710
  51. The levels of phosphorylated FAK (Tyr397 and Tyr925) were increased after overexpressing Lasp2 and were downregulated by transfecting Lasp2-siRNA. PMID: 28667800
  52. Human pluripotent stem cells promote the expression of integrin alpha6beta1, and nuclear localization and inactivation of FAK to supports stem cell self-renewal. PMID: 26930028
  53. In contrast to mice, the analysis of human olfactory bulbs revealed a late activation of FAK in advanced AAlzheimer's disease stages, whereas ERK1/2 activation was enhanced across AD staging PMID: 27498392
  54. focal adhesion kinase (FAK) transduces integrin activation and supports Human embryonic stem cell survival, substrate adhesion, and maintenance of the undifferentiated state. PMID: 27509133
  55. Data show that miR-193b, by directly targeting focal adhesion kinase (FAK), CRK-like proto-oncogene (CRKL), and methionine sulfoxide reductase A (MSRA), regulates focal adhesion signaling and ROS signaling, which play pivotal roles in liposarcomagenesis and adipogenic differentiation. PMID: 28882999
  56. PTK2 inhibition-induced sustained levels of ATG3 were able to sensitize cancer cells to DNA-damaging agents. PMID: 28103122
  57. High FAK expression is associated with Ovarian Cancer. PMID: 28933561
  58. Generally, this study indicates that miR-3173 negatively regulates PTK2 and inhibits proliferation and invasion of B-ALL cell lines. Thus, miR-3173 may represent a potential therapeutic molecule for B-ALL intervention. PMID: 29066351
  59. elevated levels of bile acid increase the tumorigenic potential of pancreatic cancer cells by inducing FXR/FAK/c-Jun axis to upregulate MUC4 expression. PMID: 27185392
  60. Osteoprotegerin facilitates pulmonary arterial hypertension pathogenesis by regulating pulmonary arterial smooth muscle cell proliferation via integrin alphavbeta3/FAK/AKT signaling pathway. PMID: 28077433
  61. the active phosphorylated form of Src (Src(pY416) ) is co-expressed in Exo with phosphorylated FAK (FAK(pY861) ), a known target site of Src, which enhances proliferation and migration. PMID: 27232975
  62. our findings identified FAK as a common aberrant protein overexpression in various subtypes of osteosarcoma. pFAK-Y397 overexpression can be used as a prognostic biomarker predicting poor OS for patients with metastatic osteosarcoma, and the expression of pFAK-Y397 differentiated good and poor responders to neoadjuvant chemotherapy. PMID: 28846700
  63. Data suggest that the cytoplasmic domain of Sdc2 is involved in regulation of expression of MMP7 in colon carcinoma/adenocarcinoma cells; induction of MMP7 involves protein kinase C gamma-mediated FAK/ERK signaling. (Sdc2 = syndecan-2; MMP7 = matrix metalloproteinase-7; FAK = focal adhesion kinase 1) PMID: 28821612
  64. High FAK expression is associated with melanoma. PMID: 28092671
  65. High FAK expression is associated with Tunneling nanotubes formation in squamous cell carcinoma. PMID: 28423494
  66. sp(2) -Iminosugar alpha-glucosidase inhibitor 1-C-octyl-2-oxa-3-oxocastanospermine inhibits breast cancer cell migration via beta1-integrin, Stim1, and FAK signaling pathways. PMID: 28145580
  67. results suggest that Kctd20 impacts proliferation and invasion of non-small cell lung cancer through enhancing Fak and Akt phosphorylation PMID: 28398603
  68. mTORC1/2 inhibition promotes reorganization of integrin/focal adhesion kinase-mediated adhesomes, induction of IGFR/IR-dependent PI3K activation, and Akt phosphorylation via an integrin/FAK/IGF1R-dependent process, mediating tumor drug resistance. PMID: 28757207
  69. Recruitment of Paxillin, Cdc42 and N-WASP is necessary for cell adhesion, migration and invasion induced by Estradiol in breast cancer cells. PMID: 27095481
  70. Type I collagen increased pancreatic ductal adenocarcinoma tumor initiating potential, self-renewal, and the frequency of Cancer stem cells through the activation of FAK. PMID: 28692661
  71. the administration of 10(-6) M retinoic acid (10-20 min) induces the activation of the migration-related proteins Moesin, FAK, and Paxillin in T-47D breast cancer cells. PMID: 27130522
  72. Study demonstrates that FAK expression, but not kinase activity alone, predominantly mediates thyroid tumor growth and metastasis. PMID: 27259715
  73. The Pyk2/STAP-2 interaction is a novel mechanism to regulate SDF-1alpha-dependent T-cell chemotaxis. PMID: 28478037
  74. overexpression of syndecan-1 confers to B-LCs an increased capacity to migrate in response to Tat, owing to a switch from a CXCR4/G-protein/Rac to a syndecan-1/alphavbeta3/pp60src/pp125FAK signal transduction pathway that depends on the formation of a complex in which syndecan-1 interacts with Tat via its HS-chains, with alphavbeta3 via its core protein ectodomain and with pp60src via its intracellular tail PMID: 27819680
  75. MUC5AC interacts with integrin beta4 that mediates phosphorylation of FAK at Y397 leading to lung cancer cell migration. PMID: 26751774
  76. Focal adhesion kinase (FAK) in platelets regulated their migration into the tumor microenvironment, and FAK-deficient platelets completely prevented the rebound tumor growth. PMID: 27064283
  77. results suggest that SOX11 promotes MCL homing and invasion and increases CAM-DR through the direct regulation of CXCR4 and FAK expression and FAK/PI3K/AKT pathway activation, contributing to a more aggressive phenotype. PMID: 28533307
  78. CXCL1 secreted by tumor-associated lymphatic endothelial cells promotes lymph node metastasis of gastric cancer through integrin beta1/FAK/AKT signaling pathway. PMID: 27832972
  79. study shows that high expression of FAK is associated with a worse OS in patients with carcinomas, but the association between FAK and prognosis varies according to cancer type PMID: 27637100
  80. FAK activity was elevated in human pancreatic ductal adenocarcinoma tissues and correlated with high levels of fibrosis and poor CD8(+) cytotoxic T cell infiltration. PMID: 27376576
  81. tyrosine kinase FAK and the laminin subunit LAMB3 as functional targets of miR-1298. Silencing of FAK or LAMB3 recapitulated the synthetic lethal effects of miR-1298 expression in KRAS-driven cancer cells. PMID: 27698189
  82. Knocking out FAK (also known as PTK2) and vinculin caused an increase in nuclear paxillin. This could be reversed by rescue with wild-type FAK but not by FAK with a mutated FAT domain, which inhibits paxillin binding. PMID: 27068537
  83. Triiodothyronine (T3) regulates actin remodeling and cell movement in breast cancer T-47D cells through the recruitment of FAK. T3 controls FAK phosphorylation and translocation at sites where focal adhesion complexes are assembled. PMID: 28050799
  84. The phosphorylation of focal adhesion kinase (FAK) was increased in spheroids compared to adherent cells, and inhibition of FAK in spheroids resulted in inhibition of the downstream mammalian target of the rapamycin (mTOR) pathway in ovarian clear cell carcinomas. PMID: 28259988
  85. SMARCE1 plays an essential role in breast cancer metastasis by protecting cells against anoikis through the HIF1A/PTK2 pathway. PMID: 27495308
  86. Data show that p63 transcription factors modulate cell migration via Src-focal adhesion kinase (FAK) signaling in foreskin keratinocytes (HFK) expressing human papilloma virus (HPV)16 E6/E7 genes. PMID: 26001294
  87. Focal adhesion kinase expression predicts neoadjuvant Chemoradiation response in rectal cancer patients PMID: 27171907
  88. FAK is tightly associated with H3K9 methylation and negatively related to the growth of tumor-repopulating cells. PMID: 28007596
  89. FAK plays a key role in regulating macrophage behavior, which underlies the chronic progression of abdominal aortic aneurysms. PMID: 27856458
  90. No associations between PTK2 SNP and skeletal muscle specific force were observed. PMID: 28251396
  91. FAK kinase activity is important in promoting influenza A virus subtypes replication by regulating its polymerase activity. PMID: 27743963
  92. Mono treatment with lexatumumab was not sufficient to induce apoptosis in pancreatic cancer cells, whereas focal adhesion kinase inhibitor PF573228 significantly sensitized lexatumumab-induced apoptosis. Western blotting analysis revealed that lexatumumab and PF573228 combination treatment increased death receptor 5 but decreased Bcl-xL expression. sed the ins PMID: 28459212
  93. NF2 regulates the interaction of FAK-p53 and MDM2-p53. PMID: 27736841
  94. Taken together, our findings suggested that HMGB1 enhances tumor cell migration ability by activating alphavbeta3/FAK through TLR4/NF-kappaB signaling, leading to metastasis of NSCLC. PMID: 27769864
  95. The main significance of this work is the discovery of EPO as a novel ligand for the HER2 receptor. Following HER2 activation, EPO induces activation of FAK and subsequent activation of beta1-integrin, via inside-out signaling. This complex results in downstream activation of ERK1/2 and a sustained up regulation of both MUC4 and the HER2 receptor PMID: 27519953
  96. The beta-arrestin1.STAM1 complex is necessary for promoting autophosphorylation of focal adhesion kinase (FAK). FAK is necessary for CXCL12-induced chemotaxis and associates with and localizes with beta-arrestin1 and STAM1 in a CXCL12-dependent manner. PMID: 27789711
  97. data suggest that activation of FAK by the interaction between CD133 and Src promotes cell migration, providing clues to understand the migratory mechanism of CD133(+) tumor cells. PMID: 27226554
  98. Focal adhesion kinase (FAK) is over-expressed in a variety of tumours, including hepatocellular carcinoma (HCC). PMID: 28067792
  99. data indicate that the beta4 integrin/FAK complex and subsequent FAK activation are essential regulators during the tumorigenicity of colon cancer PMID: 27178753
  100. monocytes infected with T. gondii can disrupt outer blood-retinal barrier, which is mediated by paracrinely activated FAK signaling. PMID: 28406972

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Involvement in disease Aberrant PTK2/FAK1 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. PTK2/FAK1 overexpression is seen in many types of cancer.
Subcellular Location Cell junction, focal adhesion, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cell cortex, Cytoplasm, cytoskeleton, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nucleus
Protein Families Protein kinase superfamily, Tyr protein kinase family, FAK subfamily
Tissue Specificity Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.
Database Links

HGNC: 9611

OMIM: 600758

KEGG: hsa:5747

STRING: 9606.ENSP00000341189

UniGene: Hs.395482

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