Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized SIRPA at 2 μg/ml can bind human CD47(CSB-MP004940HU), the EC50 of human SIRPA protein is 58.30-85.04 ng/ml.②Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.
Alternative Names
Brain Ig-like molecule with tyrosine-based activation motifs (Bit) (CD172 antigen-like family member A) (Inhibitory receptor SHPS-1) (Macrophage fusion receptor) (MyD-1 antigen) (Signal-regulatory protein alpha-1) (Sirp-alpha-1) (Signal-regulatory protein alpha-2) (Sirp-alpha-2) (Signal-regulatory protein alpha-3) (Sirp-alpha-3) (p84) (CD_antigen: CD172a) (SHP substrate 1) (SHPS-1) (BIT) (MFR) (MYD1) (PTPNS1) (SHPS1) (SIRP)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
31-370aa
Target Protein Sequence
EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER
Tag Info
C-terminal 6xHis-Myc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant human tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) is an active protein expressed from mammalian cells, with a C-terminal 6xHis-Myc-tag. Its expression region is the DNA fragment encoding the amino acid residues 31-370 of the human SIRPA protein. The purity of this SIRPA protein is greater than 95% measured by SDS-PAGE. This recombinant SIRPA protein migrated to the band with a molecular weight of approximately 50 kDa on the gel. Its endotoxin level is less than 1.0 EU/ug determined by the LAL method. And its bioactivity has been validated in the ELISA and LSPR assay. In the functional ELISA, this SIRPA protein binds to the human CD47, with an EC50 constant of 58.30-85.04 ng/ml. In the LSPR assay, the SIRPA protein captured on the COOH ship binds to the human CD47 protein with an affinity constant of 19.1 nM. It is in stock now.
SIRPA binds to its ligand CD47, initiating a signaling cascade that results in the inhibition of phagocytosis. It also suppresses infection by various viruses that enter via acidic compartments, including Zika, SARS-CoV-2, and Ebola.