SIRPA

SIRPA is a gene providing an instruction of making a protein named tyrosine-protein phosphatase non-receptor type substrate 1 (usually abbreviated as SIRPA). SIRPA is also known as CD172a, SHPS-1, P84, BIT, and its glycosylation structure is species- and tissue-specific. It is a plasma membrane glycoprotein mainly expressed on the surface of nerve cells and myeloid cells, and SIRPA is often expressed as an inhibitory receptor in myeloid cells. SHP-1 and SHP-2 are non-transmembrane protein tyrosine phosphatases that regulate macrophage proliferation and activation in multiple ways. SIRPA activates tyrosine phosphorylation in the cytoplasm, and then recruits and activates tyrosine phosphatases (SHP1, SHP-2) with SH2 domains. SIRPA can act as a substrate protein for SHP1/SHP-2 to dephosphorylate , which in turn inhibits protein tyrosine kinase (PIK)-dependent cellular activation and inhibits immune cell activation.

The process of inhibiting phagocytosis of macrophages by SIRPa requires the binding of ligands. Currently, the known ligands of SIRPa include CD47, alveolar surfactant protein A (SP-A), and alveolar surfactant protein D (SP-D). Among them, SIRPa/CD47 binding is important during macrophage phagocytosis.

Popular Product

Recombinant Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA),partial (Active) (CSB-MP021334HU)

Validated Data

Purity determined
by
SDS-PAGE

Greater than 95% as determined by SDS-PAGE.

The binding activity of SIRPA-CD47 validated by functional ELISA

Immobilized SIRPA at 2 μg/ml can bind human CD47 (CSB-MP004940HU), the EC₅₀ of human SIRPA protein is 58.30-85.04 ng/ml.

The binding activity of SIRPA-CD47 validated
by LSPR

Human SIRPA protein captured on COOH chip can bind Human CD47 protein with an affinity constant of 19.1 nM as detected by LSPR Assay.