SIRPA, also known as Signal regulatory protein alpha, and by other names such as Sirp-alpha-3p84 or CD172a, is a type I transmembrane protein and a member of the immunoglobulin superfamily. It is primarily expressed on the surface of myeloid cells such as macrophages, monocytes, and dendritic cells, as well as on neural cells. The mechanism of action for SIRPA involves binding to ligands such as CD47, transmitting inhibitory signals that suppress phagocytosis by macrophages, thus playing a crucial role in immune regulation. The intracellular domain of SIRPA contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which regulate cellular signaling pathways through dephosphorylation.
SIRPA is involved in the regulation of various physiological and pathological processes, including immune responses, cell phagocytosis, central nervous system development, and directed hematopoietic stem cell transplantation. Through its interaction with CD47, SIRPA can inhibit macrophage phagocytosis of target cells, a mechanism exploited by tumor cells to evade the immune system. Consequently, SIRPA is a potential target in cancer immunotherapy, particularly when used in combination with PD-1-PD-L1 blockers, which may have significant implications for the treatment of certain types of cancer.
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized SIRPA at 2 μg/ml can bind human CD47(CSB-MP004940HU), the EC50 of human SIRPA protein is 58.30-85.04 ng/ml.
Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.
SIRPA Recombinant Monoclonal Antibody (CSB-RA443117A0HU)
Validated Data
IHC image of CSB-RA443117A0HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.13% DAB.
IHC image of CSB-RA443117A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.13% DAB.
Overlay Peak curve showing U937 cells surface stained with CSB-RA443117A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA) ELISA kit (CSB-EL021334HU)
Validated Data
Code: CSB-EL021334HU
Size: 96T,5×96T,10×96T
Sensitivity: 0.039 ng/mL
Detection Range: 0.156 ng/mL-10 ng/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following SIRPA reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
SIRPA Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA021334GA01HU | SIRPA Antibody | Human | ELISA,WB |
CSB-PA021334LB01HU | SIRPA Antibody, HRP conjugated | Human | ELISA |
CSB-PA021334LC01HU | SIRPA Antibody, FITC conjugated | Human | |
CSB-PA021334LD01HU | SIRPA Antibody, Biotin conjugated | Human | ELISA |
CSB-PA021334LA01HU | SIRPA Antibody | Human | ELISA, IHC, IF |
CSB-PA004094 | SIRPA Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-RA443117A0HU | SIRPA Recombinant Monoclonal Antibody | Human | ELISA, IHC, FC |
SIRPA Proteins for Homo sapiens (Human)
SIRPA Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-CF021334BO | Recombinant Bovine Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) | in vitro E.coli expression system |
CSB-YP021334BO1 CSB-EP021334BO1 CSB-BP021334BO1 CSB-MP021334BO1 CSB-EP021334BO1-B |
Recombinant Bovine Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SIRPA Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-CF021334RA | Recombinant Rat Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa) | in vitro E.coli expression system |
CSB-YP021334RA1 CSB-EP021334RA1 CSB-BP021334RA1 CSB-MP021334RA1 CSB-EP021334RA1-B |
Recombinant Rat Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SIRPA Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF021334MO | Recombinant Mouse Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa) | in vitro E.coli expression system |
CSB-YP021334MO1 CSB-EP021334MO1 CSB-BP021334MO1 CSB-MP021334MO1 CSB-EP021334MO1-B |
Recombinant Mouse Tyrosine-protein phosphatase non-receptor type substrate 1 (Sirpa), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SIRPA ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL021334HU | Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 0.039 ng/mL |