Recombinant Mouse Rho-associated protein kinase 1(Rock1) ,partial

Code CSB-YP020058MO
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Source Yeast
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Code CSB-EP020058MO
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Source E.coli
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Code CSB-EP020058MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020058MO
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Source Baculovirus
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Code CSB-MP020058MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Rock1
Uniprot No. P70335
Alternative Names Rock1; Rho-associated protein kinase 1; EC 2.7.11.1; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Promotes keratinocyte terminal differentiation (By similarity). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.
Gene References into Functions
  1. by differentially affecting CyPA and Bsg expressions, ROCK1 protects and ROCK2 jeopardizes the heart from pressure-overload heart failure with postcapillary pulmonary hypertension, for which celastrol may be a promising agent. PMID: 29987023
  2. Data indicate that Rho-associated protein kinase (ROCK)-dependent intermittent T cell migration regulates tissue-sampling during acute lung injury. PMID: 29044117
  3. findings are the first to show that astrocytes exposed to MeHg showed increased cleavage/activation of ROCK-1, which was independent of the small GTPase RhoA PMID: 29428217
  4. the transcription of Rho-associated coiled-coil containing protein kinase 1 (ROCK1), which phosphorylates Drp1 at Ser616, was shown by luciferase assay to be directly regulated by FOXO1. These findings suggested that FOXO1 is critical to preserve mitochondrial quantity and function in ECs, and FOXO1 may serve as a therapeutic target for microvascular complications of diabetes. PMID: 29654945
  5. A specific inhibitor of ROCK, Y-27632, was used to examine the role of ROCK in mouse colitis models. ROCK1 and ROCK2 were silenced respectively using RNA interference in Caco-2 cells. The expression of tight junction proteins and the downstream molecules of ROCK were assessed PMID: 29115372
  6. GTP-RhoA and ROCK1 expression levels were markedly increased in a time-dependent manner in the ears and lungs of mice treated with penicillin. PMID: 27619816
  7. Irisin replenishment in mCaROCK1 mice partially reversed insulin resistance. PMID: 27411515
  8. MicroRNA-146a suppresses ROCK1 allowing hyperphosphorylation of tau in Alzheimer's disease PMID: 27221467
  9. Fasudil exhibited protective effects on smoke exposure induced cognitive deficits which might involve with the regulation of Rho/ROCK/NF-kappaB pathways. PMID: 27791202
  10. Dexamethasone up-regulates ROCK1/2 activity promoting migration, invasion and metastasis of melanoma cells. PMID: 28923399
  11. mDia induces circumferential actin filaments around the edge of the synaptic cleft, which contract the presynaptic terminals in a ROCK-dependent manner. PMID: 27880913
  12. SNRK in cardiomyocytes is responsible for maintaining cardiac metabolic homeostasis, which is mediated in part by ROCK, and alteration of this homeostasis influences cardiac function in the adult heart. PMID: 27780848
  13. we uncovered cell state plasticity and adhesion dynamics regulated by Ror2, which influenced Ras Homology Family Member A (RhoA) and Rho-Associated Coiled-Coil Kinase 1 (ROCK1) activity downstream of Dishevelled-2 (Dvl2). PMID: 28650466
  14. Data indicate that oxidative stress in diabetes causes a decrease in miR-133a expression leading to an increase in RhoA/Rho kinase pathway and muscle contraction. PMID: 28678840
  15. that reduction of ROCK1 diminishes amyloid-beta levels by enhancing Amyloid beta-Protein Precursor protein degradation PMID: 27246255
  16. the cross-talk between canonical and non-canonical signaling pathways of Wnt3A, which induces GSK-3beta phosphorylation and beta-catenin accumulation through RhoA and ROCK activation. PMID: 27575935
  17. Inhibition of ROCK1 largely blocked Endothelial-to-mesenchymal transition and the increase in endothelial permeability under this high-glucose condition, but overexpression of ROCK1 induced these changes. PMID: 26842599
  18. While the loss of either Rock1 or Rock2 had no negative impact on tumorigenesis in mouse models of non-small cell lung cancer and melanoma, loss of both blocked tumor formation, as no tumors arise in which both Rock1 and Rock2 have been genetically deleted. PMID: 26765561
  19. YQFM ameliorates the OGD-induced brain microvascular endothelial cell barrier disruption associated with the NF-kappaB/p65 and ROCK1/MLC signaling pathways. PMID: 26915982
  20. Extracellular vesicle-encapsulated caspase-3 was largely responsible for the alveolar macrophage activation via the ROCK1 pathway. PMID: 26658190
  21. In experimental autoimmune encephalomyelitis (EAE), ROCK activity was also increased in serum, spleen, brain and spinal cord. Neuron injury with scratch and TNF-alpha stimulation induced the up-regulation of ROCK activity. PMID: 26481340
  22. indicates that the up-regulation of ROCK1 activity in RhoA-deleted macrophages and macrophage phenotype/polarity are dependent on non-apoptotic Caspase-3 PMID: 26875770
  23. Rho-kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closure. PMID: 26040287
  24. Results suggest that Rho-associated, coiled-coil containing protein kinase (ROCK) inhibitors fasudil may be an emerging therapeutic option for targeting cancer vasculogenic mimicry (VM). PMID: 25934709
  25. doxorubicin induces caspase activation, necrosis, and actin cytoskeleton alterations largely through ROCK1-dependent and oxidative stress-independent pathways PMID: 26134406
  26. A very rapid reduction in p27-phospho-Ser10 levels at the onset of atherogenesis, which contributes to early plaque build-up through RhoA/ROCK-induced integrin expression, is reported. PMID: 25908026
  27. RhoA and ROCK mediate histamine-induced vascular leakage and anaphylactic shock. PMID: 25857352
  28. ROCK1-dependent changes in hyaluronan may contribute to ROCK1's role in O3-induced AHR, the role of ROCK2 is downstream of inflammation, likely at the level of airway smooth muscle contraction. PMID: 26276827
  29. The results showed that ROCK was essential for early embryo cleavage and blastocyst formation, possibly through a conserved ROCK-LIMK1/2-actin pathway, during mouse embryo development. PMID: 24562870
  30. The role of Rho-kinase in leukocytes during ischemia/reperfusion injury. PMID: 24638037
  31. Proper inner cell mass morphology is dependent on ROCK activity and is crucial for fetal development. PMID: 23946538
  32. Data indicate that transient receptor potential melastatin subtype 8 (TRPM8) activation by menthol attenuated vasoconstriction via RhoA/Rho kinase pathway inhibition in wild-type mice, but the effect was absent in TRPM8(-/-) mice. PMID: 24637663
  33. Adipose ROCK1 isoform plays an inhibtory role for the regulation of insulin sensitivity in diet-induced obesity in vivo. PMID: 24326423
  34. Rho kinase inhibition enhances the survival of newborn neurons in the dentate gyrus. PMID: 23707981
  35. ROCK1(-/-) MEFs showed significant preservation of both focal adhesions and adherens junctions. PMID: 23598717
  36. Data from transgenic/knockout mice suggest loss of Rock1 in AgRP (agouti-related peptide) neurons of arcuate nucleus leads to decrease in energy expenditure/locomotor activity independent of change in food intake and thus leads to obesity/adiposity. PMID: 23885017
  37. the two isoforms of Rho-kinase distribute differentially to accomplish their specific functions PMID: 22986902
  38. Rock1 displays specific control of endothelial cell survival. PMID: 22934846
  39. ROCK1 is involved in destabilizing actin cytoskeleton through regulating MLC2 phosphorylation and peripheral actomyosin contraction. PMID: 23392171
  40. ROCK inhibition diminishes most microglial activation properties, such as the increased cell body size, number of filopodial processes and size of Golgi apparatus, after MPTP insult. PMID: 23139861
  41. data identify ROCK1 as a key regulator of leptin action on energy homeostasis. PMID: 22941110
  42. Rock is thus essential for survival of neural crest cells that form the craniofacial region. PMID: 22629443
  43. ROCK1 functions as a physiologic regulator of p53 under conditions of erythroid stress. PMID: 22889758
  44. Rho-kinase signaling constitures a key feature in streptococcal M1 protein induced acute lung injury. PMID: 22433673
  45. our overall results reveal a model whereby CD1d-mediated Ag presentation is negatively regulated by ROCK1 and ROCK2 via its effects on the actin cytoskeleton. PMID: 22798677
  46. Data suggest that small artery dysfunction in diabetes involves suppression of extracellular glucose dependency of vasoconstriction under hyperglycemic conditions; this dysfunction appear to involve reduced Rho kinase expression. PMID: 22465314
  47. a novel mechanism in which miR-148a positively regulates myogenic differentiation via ROCK1 down-regulation. PMID: 22547064
  48. Regulation of glucose transport by ROCK1 differs from that of ROCK2 and is controlled by actin polymerization. PMID: 22355071
  49. conclude that ROCKDelta1 is a novel fibrotic factor. Activation of TGFbeta1 and NF-kappaB signaling contributes to the Rho kinase-mediated pathological fibrosis PMID: 22278938
  50. Mitochondrial fission triggered by hyperglycemia is mediated by ROCK1 activation in podocytes and endothelial cells. PMID: 22326220
  51. ROCK1 may have an important role in the process of myocardialization of the proximal right outflow tract septum. PMID: 22088464
  52. The role of ROCK within the specification and differentiation of all three germ layers was examined. PMID: 22140430
  53. ROCK1-directed basement membrane positioning coordinates epithelial tissue polarity. PMID: 22186730
  54. Both ROCK1 and ROCK2 are important in regulating allergic airway responses. PMID: 21565918
  55. ROCK isoforms function redundantly during embryogenesis and play a critical role in vascular development. PMID: 21895889
  56. Using siRNA, knockdown of both ROCK1 and ROCK2 was required to promote NPC migration and morphological changes PMID: 21732490
  57. We show constitutive activation of Rho kinase in cells bearing oncogenic forms of KIT, FLT3, and BCR-ABL, which is dependent on PI3K and Rho GTPase. PMID: 21907926
  58. A novel role has been uncovered for ROCK1 in amelogenesis. PMID: 21792909
  59. p120-catenin regulates growth of metastatic lobular carcinoma through Rock1-mediated anoikis resistance PMID: 21747168
  60. The decrease in Rho kinase-dependent contractions in P18 diabetic mice coincided with reduced RhoA and Rho kinase expression relative to nonpregnant diabetic mice PMID: 21558549
  61. Report a willin(FRMD6)/Par3-aPKC-ROCK pathway that controls epithelial apical morphology. PMID: 21685893
  62. calcium channel-induced Ca(2+) release has a major role in tonic vascular smooth muscle contractility because it links membrane depolarization and Ca(2+) channel activation with metabotropic Ca(2+) release and sensitization (RhoA/ROCK stimulation) PMID: 21493898
  63. Data suggest that in addition to regulating tumorigenesis through modulation of the phosphoproteome, Rho kinase signaling also contributes to the regulation of the tumor transcriptome. PMID: 21471517
  64. Heparin inhibits pulmonary artery smooth muscle cell proliferation through GEF-H1/RhoA/ROCK/p27 signaling pathway. PMID: 20558775
  65. The present study investigated whether ROCK1 deficiency can prevent the transition from hypertrophy to heart failure. PMID: 20709073
  66. Rho-kinase signalling regulates TNF-alpha and CXC chemokine formation as well as lipid peroxidation in the reperfused colon. PMID: 20593289
  67. Expression of MT1-MMP may be anti-tumorigenic in keratinocytes by promoting cellular aggregation. PMID: 20605790
  68. Data show that silencing of ROCK-1 or ROCK-2 in cells seeded at 2x10(3)/cm(2) did not change renewal factors. PMID: 20169147
  69. These results suggest that sphingosine 1-phosphate (S1P) is capable of altering pulmonary vascular tone in mouse lung through S1P2 receptor-dependent Rho-kinase activation-mediated vasoconstriction. PMID: 20435688
  70. Suggest that RAGE-dependent acceleration of atherosclerosis in ApoE-null mice is dependent on the action of the ROCK1 (rho-associated protein kinase 1) branch of the transforming growth factor-beta pathway. PMID: 20133903
  71. alpha(1)-adrenergic receptor smooth muscle contraction shows higher dependency on Rho-kinase activity, suggesting down-regulation of the mechanism different from Rho/Rho kinase signaling in the aorta prior to atherosclerosis PMID: 19755789
  72. This study identifies a specific signaling pathway, mediated by Rhoa and Rock1, which determines cell fate by promoting stress granules formation or initiating apoptosis during stress. PMID: 20004716
  73. ROCK and mDia1 antagonize in Rho-dependent Rac activation in Swiss 3T3 fibroblasts. PMID: 12021256
  74. ROCK I-induced stress fiber formation and phosphorylation of the ROCK I target myosin light chain phosphatase is regulated by RhoE PMID: 12773565
  75. a novel mechanism for coupling cytoplasmic p21(Cip1) to the control of actin polymerization by compromising the Rho/ROCK/LIMK/cofilin pathway by oncogenic Ras. PMID: 14559914
  76. Signaling through RhoA and p160ROCK is important in TGF-beta inhibition of cell proliferation. PMID: 14657354
  77. the RhoA/ROCK signaling pathway is a novel and important regulator of chondrocyte proliferation and differentiation PMID: 14726536
  78. Rho-kinase is substantially involved in the pathogenesis of LV remodeling after MI associated with upregulation of proinflammatory cytokines. PMID: 15096457
  79. inactivation of Rho/ROCK signaling is a prerequisite for FKHR nuclear translocation and myoblast fusion in C2C12 cells PMID: 15322110
  80. Plays an essential role in endothelial cell differentiation and migration during endocardial cushion development. PMID: 15464581
  81. a RhoA-ROCK-MEKK1-JNK pathway and a MEKK1-p38 pathway are Smad-independent mechanisms in the transmission of activin signals PMID: 15601830
  82. RhoA/ROCK signaling suppresses chondrogenesis through the control of Sox9 expression and actin organization. PMID: 15665004
  83. Rock1 regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles. PMID: 15753128
  84. ROCK I phosphorylates multiple residues on RhoE. PMID: 15775972
  85. These results suggest that ROCK-I and ROCK-II cooperatively regulates the assembly of actin bundles essential for closure of the eyelid and ventral body wall in mouse embryos PMID: 16098146
  86. ROCK has a role in ischemia-induced endothelial dysfunction during stroke PMID: 16141422
  87. ROCK1 is critical for the development of cardiac fibrosis, but not hypertrophy, suggesting that signaling pathways leading to the hypertrophic and profibrotic response of the heart are distinct. PMID: 16260635
  88. role in increase phosphorylation of myosin light chain and stress fiber formation PMID: 16613852
  89. ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. PMID: 16675849
  90. Cyclin D1 promotes cellular motility through inhibiting ROCK signaling and repressing the metastasis suppressor TSP-1. PMID: 16705174
  91. Integrin cytoplasmic domain-associated protein-1 (ICAP-1) binds ROCK at the cell membrane. PMID: 16741948
  92. ROCK-1-dependent caspase-3 activation was coupled with the activation of PTEN and the subsequent inhibition of protein kinase B (Akt) activity, all of which was attenuated by siRNA directed against ROCK-1 expression. PMID: 16983089
  93. the induction of Hic-5 expression in response to TGFbeta1 was shown to be a RhoA/ROCK I-dependent process PMID: 17299801
  94. This review, based on recent molecular, cellular, and animal studies, focuses on the current understanding of ROCK signaling in the regulation of apoptosis and highlights new findings from recently generated ROCK-deficient mice. PMID: 17347801
  95. ROCKI/II and Rac/Cdc42 are required for both neural crest-derived cell migration and axon growth in the developing gut. PMID: 17524389
  96. Loss of p53 promotes Rhoa-Rock1-dependent cell migration and invasion in 3D matrices. PMID: 17606864
  97. inhibition of ROCK-II, not ROCK-I, enhances adipogenesis accompanied by the up-regulation of adipogenic transcription factors PMID: 17681946
  98. The RhoA/ROCK pathway negatively regulates membrane FasL expression in B16F10 melanoma cells. PMID: 18084615
  99. Study establishes for the first time a role for ROCK1 in cardiac dilation and contractile dysfunction. PMID: 18178218
  100. Data suggest that ROCK1 in bone marrow-derived cells mediates neointima formation following vascular injury and suggest that ROCK1 may represent a promising therapeutic target in vascular inflammatory diseases. PMID: 18414683

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Subcellular Location Cytoplasm, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Golgi apparatus membrane, Peripheral membrane protein, Cell projection, bleb, Cytoplasm, cytoskeleton, Cell membrane, Cell projection, lamellipodium, Cell projection, ruffle
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family
Tissue Specificity Highly expressed in brain, heart, lung, liver, stomach, spleen, kidney, testis, muscle, embryo and placenta.
Database Links

KEGG: mmu:19877

STRING: 10090.ENSMUSP00000069549

UniGene: Mm.6710

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