Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Tert

Uniprot No.

O70372

Research Area

others

Alternative Names

Tert; Telomerase reverse transcriptase; EC 2.7.7.49; Telomerase catalytic subunit

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

595-928aa

Target Protein Sequence

EVRHHQDTWLAMPICRLRFIPKPNGLRPIVNMSYSMGTRALGRRKQAQHFTQRLKTLFSMLNYERTKHPHLMGSSVLGMNDIYRTWRAFVLRVRALDQTPRMYFVKADVTGAYDAIPQGKLVEVVANMIRHSESTYCIRQYAVVRRDSQGQVHKSFRRQVTTLSDLQPYMGQFLKHLQDSDASALRNSVVIEQSISMNESSSSLFDFFLHFLRHSVVKIGDRCYTQCQGIPQGSSLSTLLCSLCFGDMENKLFAEVQRDGLLLRFVDDFLLVTPHLDQAKTFLSTLVHGVPEYGCMINLQKTVVNFPVEPGTLGGAAPYQLPAHCLFPWCGLLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

54.1kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 595-928 form the expressed segment for recombinant Mouse Tert. The theoretical molecular weight of the Tert protein is 54.1 kDa. This Tert recombinant protein is manufactured in e.coli. The Tert coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant Tert protein in following stages of expression and purification.

The telomerase reverse transcriptase (TERT) is a crucial enzyme involved in maintaining telomere length, which is essential for chromosomal stability and cellular lifespan. TERT is a catalytic subunit of the telomerase complex, responsible for adding telomeric DNA repeats to the ends of chromosomes during DNA replication. This process counteracts the gradual shortening of telomeres that occurs with each cell division. In mice, TERT plays a fundamental role in preventing premature cellular senescence and supporting tissue homeostasis. The enzyme is particularly active in stem cells and highly proliferative cells, contributing to their ability to undergo numerous cell divisions. Research areas related to TERT include aging, cancer, and regenerative medicine, as its activity is tightly linked to cellular processes influencing both normal development and pathological conditions. Understanding the regulation and function of TERT holds significant implications for potential therapeutic strategies targeting age-related diseases and cancer.

Target Background

Function

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

Gene References into Functions

Par-4 activation and binding to TERT are key steps required for inducing the apoptosis of islet beta cells under high-glucose/fatty acid conditions in type 2 diabetes. PMID: 30186877
Tert-deficient but not Terc-deficient mice develop hepatocyte injury and frank steatosis when challenged with liquid high-fat diet. PMID: 28741793
identification of a subset of hepatocytes that expresses high levels of telomerase and show that this hepatocyte subset repopulates the liver during homeostasis and injury PMID: 29618815
These preclinical data in mouse models and human cells provide a strong rationale for the development of pharmacological approaches to target BMI1-mediated mitochondrial regulation and protection from DNA damage to sustain the regenerative potential of the skeletal muscle in conditions of chronic muscle wasting. PMID: 28757168
Reactivation of Tert in the hippocampus was sufficient to normalize the depressive but not the aggressive behaviors of Tert(-/-) mice. Conversely, re-expression of Tert in the medial prefrontal cortex (mPFC) reversed the aggressive but not the depressive behavior of Tert(-/-) mice. PMID: 27300262
Nrf2-driven TERT regulates pentose phosphate pathway in glioblastoma. PMID: 27148686
TERT has a role in neointima formation through epigenetic regulation of proliferative E2F1 target gene expression in smooth muscle cells. PMID: 27932351
these findings support a model in which gain of TERT function modulates mTORC1 activity and induces autophagy. PMID: 27545609
Regarding extratelomeric activities, our results showed a decrease of 64, 38 and 25% in the transcription of c-Myc, Cyc-D1 and TERT, respectively (p<0.05) after AZT treatment. Furthermore, we found an effect on cell migration, reaching an inhibition of 48% (p<0.05) and a significant passage-dependent increase on cell doubling time during treatment PMID: 27633795
Results suggest that in mature Purkinje neurons, TERT is present both in the nucleus and in mitochondria, where it may participate in adaptive responses of the neurons to excitotoxic and radiation stress PMID: 26374457
Wnt10a/beta-catenin signaling pathway is able to exacerbate keloid cell proliferation and inhibit the apoptosis of keloid cells through its interaction with TERT. PMID: 27771714
This study reports the characterisation of two novel mouse TERT splice variants, Ins-i1[1-102] (Insi1 for short) and Del-e12[1-40] (Dele12 for short) that have not been previously described. Insi1 represents an in-frame insertion of nucleotides 1-102 from intron 1, encoding a 34 amino acid insertion at amino acid 73. PMID: 26787169
TERT may promote gastric cancer metastasis through the TERT-miR-29a-ITGB1 regulatory pathway. PMID: 26903137
TERT switches macrophages towards M1 phenotype by regulating NF-kappaB signaling, but has limited effect on M2 macrophages polarization in vitro. PMID: 26725521
The results suggest that the mouse endometrial epithelium and vasculature are foci of stem/progenitor activity expressing mTert. PMID: 26740067
miR-195 overexpressed in old mesenchymal stem cells (OMSCs) induces stem cell senescence deteriorating their regenerative ability by directly deactivating telomerase reverse transcriptase (Tert), and abrogation of miR-195 can reverse stem cell aging. PMID: 26390028
These findings indicate that telomerase gene therapy represents a novel therapeutic strategy to treat aplastic anemia provoked or associated with short telomeres. PMID: 26903545
findings identified a key role for TERT in fibroblast proliferation and survival essential for pulmonary fibrosis PMID: 26555817
miR-512-5p suppresses tumor growth by targeting TERT in telomerase positive head and neck squamous cell carcinoma in vitro and in vivo. PMID: 26258591
Telomerase deficiency triggers alveolar stem cell replicative senescence-associated low-grade inflammation. PMID: 26518879
high telomerase expression is a fundamental characteristic of germline stem cells. PMID: 26584619
data suggest that S1P binding to hTERT allosterically mimicks phosphorylation, promoting telomerase stability and hence telomere maintenance, cell proliferation, and tumor growth. PMID: 26082434
Telomerase may direct Pol I transcription in oncogenic and regenerative hyperproliferation. PMID: 25118183
Tert expression confers cardioprotection in the adult mouse heart after acute myocardial infarction. PMID: 25519492
TERT is a regulator of MYC stability in cancer. Reactivation of TERT, a direct transcriptional MYC target in tumors, provides a feed-forward mechanism to potentiate MYC-dependent oncogenesis. PMID: 25893605
Pharmacologically relevant doses of atorvastatin resulted in a 6-fold increase of telomerase activity in mouse PBMCs and CD4 T cells. Transgenic GFP-mTert reporter mice had 30% to 15% less telomerase-positive lymphocytes during the first 5 months of age. PMID: 25127175
Studies indicate that reverse transcriptase (RT) enzyme highly expressed in mouse embryos and mouse and human cancer cells and repressed in somatic differentiated healthy cells. PMID: 25586649
The overexpression of Zfp637 markedly increases mTERT expression and telomerase activity, maintains telomere length, and inhibits H2O2 and D-galactose-induced senescence. PMID: 25032857
Telomerase exerts telomere-independent effects on pulmonary artery smooth muscle cell growth in pulmonary hypertension. PMID: 25550449
These findings establish a functional link between endoplasmic reticulum stress and telomerase. PMID: 24119029
TERT, combined with beta-catenin and BRG1, serves as a transcriptional complex, which binds the FAS ligand (FASL) promoter to upregulate FASL expression, leading to an elevated immunomodulatory function. PMID: 24401839
These data indicate that TERT plays an extratelomeric role in the reprogramming process, but its function is dispensable. PMID: 24733392
Once HIV production had reached a peak (7 dpi), the telomerase activity decreased, showing levels similar to those of noninfected cells PMID: 24254728
Overexpression of TERT in mesenchmal stem cells resulted in increased cell proliferation. PMID: 22884695
TERT expression was up-regulated by a histone deacetylase inhibitor, while the induction of TERT in lung fibroblasts was associated with the binding of acetylated histone H3K9 to the TERT promoter region. PMID: 23526226
calorie restriction attenuates telomere erosion associated to aging and that synergizes with TERT over-expression in increasing "health span" and extending mouse longevity PMID: 23349740
prepared two mutant forms of the PhSurv-PhTERT tandem with two or four Sp1 sites removed from the distal "long" PhSurv promoter PMID: 23056318
Study identified the cells responsible for cardiac telomerase activity, demonstrates a significant diminution with age. PMID: 22919071
mir498 has a role in 1,25-Dihydroxyvitamin D3 suppression of telomerase expression and cancer growth PMID: 23055531
Data show that AUF1 binds and strongly activates the transcription promoter for telomerase catalytic subunit Tert. PMID: 22633954
Essential role for telomerase in chronic myeloid leukemia induced by BCR-ABL in mice. PMID: 22408137
telomerase plays a role in the aging of nondividing cells PMID: 22533433
findings show beta-catenin regulates Tert expression through interaction with Klf4, a core component of the pluripotency transcriptional network; beta-Catenin binds to the Tert promoter in a mouse intestinal tumor model PMID: 22723415
Data provide evidence that telomere dysfunction plays a critical role in prostate cancer initiation and progression, permitting acquisition of and selection for cancer-relevant genomic events upon telomerase reactivation. PMID: 22341455
Silencing transgenic TERT expression or inhibiting Wnt signaling through systemic expression of the Wnt inhibitor Dkk1 in either TERT transgenic mice or in a mouse model of HIVAN results in marked normalization of podocytes PMID: 22138751
Selenium and benzene can upregulate the telomerase activity in mouse lymphocytes in vivo. PMID: 19080380
Hippocampal telomerase is involved in the modulation of depression-related behaviors, possibly by regulating adult neurogenesis. PMID: 21865469
Primitive hematopoietic Tert(-/-) cells lacking telomerase activity exhibit signs of enhanced DNA damage. PMID: 21730353
Results indicate that both telomerase reverse transcriptase and telomerase RNA are haploinsufficient and that their deficiency leads to telomere shortening, which limits tissue renewal. PMID: 21464209
The results of this study provide the first experimental evidence that telomere shortening, despite impairing adult neurogenesis and maintenance of post-mitotic neurons, can slow down the progression of amyloid plaque pathology in Alzheimer's disease. PMID: 21672962

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Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Chromosome, telomere. Cytoplasm. Nucleus, PML body.

Protein Families

Reverse transcriptase family, Telomerase subfamily

Tissue Specificity

High activity in intestine, liver and testis, moderate in lung, very low in muscle, heart and brain.

Database Links

KEGG: mmu:21752

STRING: 10090.ENSMUSP00000022104

UniGene: Mm.10109

Code	CSB-EP023391MO
Abbreviation	Recombinant Mouse Tert protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Telomerase reverse transcriptase (Tert), partial

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