Code | CSB-RA616025A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The process of producing the CCND1 recombinant monoclonal antibody is intricate and multi-step. Initially, the CCND1 monoclonal antibody is collected and its genetic sequence is analyzed. A vector carrying the CCND1 monoclonal antibody gene is then constructed and incorporated into a host cell line for culturing. The CCND1 monoclonal antibody is generated using a synthetic peptide sourced from human CCND1 as an immunogen. Finally, the CCND1 recombinant monoclonal antibody is purified via affinity chromatography to ensure high purity. The antibody's specificity is then verified using ELISA and WB assays, which examine its ability to accurately identify CCND1. It detects human and mouse CCND1 proteins.
CCND1 protein is a regulatory protein that plays a critical role in cell cycle progression and cell differentiation. In response to extracellular signals, CCND1 is synthesized and binds to CDK4 or CDK6 to form an active complex that phosphorylates and inactivates the retinoblastoma protein (RB), leading to the release of E2F transcription factors thus promoting the expression of genes required for DNA replication and cell cycle progression. CCND1 promotes the differentiation of various cell types, including osteoblasts, adipocytes, and neurons, by regulating the activity of Runx2, PPARgamma, and CREB, respectively. Dysregulation of CCND1 can contribute to the development and progression of cancer.
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