CCND1 Antibody

Datasheet
Code CSB-PA004811KA01HU
Product Type Rabbit Anti Human PolyClonal Antibody
Size US$398
Uniprot No. P24385
Image
  • Western blot analysis of MCF-7 cell lysate ,using Cyclin D1 antibody.
Immunogen A synthetic peptide of Human Cyclin D1
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Tested Applications ELISA,WB,IHC,ICC,IP;WB:1:500-1:1000,IHC:1:50-1:100,ICC:1:50-1:100,IP:1:20-1:50
Relevance Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation.
Storage Buffer Store at -20°C or -80°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Isotype IgG
Alias CCND1;BCL1;D11S287E;PRAD1;U21B31;G1/S-specific cyclin-D1;BCL-1 oncogene ;PRAD1 oncogene
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Research Area Cell Cycle
Intended Use For research use only. Not for human, diagnostic or therapeutic use.
Target Names CCND1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Details This protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
HGNC 1582
RGD 68384
MGI 88313
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Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Involvement in disease Multiple myeloma (MM)
Subcellular Location Nucleus, Cytoplasm, Membrane
Protein Families Cyclin family, Cyclin D subfamily
Database Links

HGNC: 1582

OMIM: 168461

KEGG: hsa:595

STRING: 9606.ENSP00000227507

UniGene: Hs.523852

Pathway Hedgehog signaling pathway
Hippo signaling pathway
Jak-STAT signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Wnt signaling pathway
Focal adhesion
Cell cycle
Cellular senescence
AGE-RAGE signaling pathway in diabetic complications
AMPK signaling
Apelin signaling pathway
FoxO signaling pathway

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