Your Good Partner in Biology Research
Your Good Partner in Biology Research
| Code | CSB-EP004811HU |
| Size |
$1812Purchase it in Cusabio online store (only available for customers from the US) |
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| Description |
This protein is a recombinant Human CCND1 protein with N-terminal 6xHis tag. Its purity is 90% determined by SDS-PAGE. The production includes 5 steps. 1, obtain the cDNA from the respective mRNA; 2, Select expression vector to insert the CCND1 sequence and get the recombinant plasmid of CCND1; 3, Clone the recombinant plasmid into E.coli; 4, Express the CCND1 protein in the E.coli cell; 5, Protein purification. And finally, the desired recombinant protein was obtained. In addition, the activity has been tested. CCND1 is a gene encoding regulatory component of the cyclin D1-CDK4 (DC) complex called G1/S-specific cyclin-D1 (also known as B-cell lymphoma 1 protein (BCL-1), Cyclin D1). CCND1 protein is involved in multiple biological processes, including cell division, fat cell differentiation, mitotic G1 DNA damage checkpoint signaling, regulation of cell cycle, Wnt signaling pathway, etc. Increasing evidence indicates that Cyclin D1 is important for the development and progression of several cancers including those of the breast, esophagus, bladder, and lung. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | CCND1 |
| Uniprot No. | P24385 |
| Research Area | Cell Cycle |
| Alternative Names | AI327039; B cell CLL/lymphoma 1; B cell leukemia 1; B cell lymphoma 1 protein; B-cell lymphoma 1 protein; BCL 1; BCL-1; BCL-1 oncogene; BCL1; BCL1 oncogene; ccnd1; CCND1/FSTL3 fusion gene; CCND1/FSTL3 fusion gene; included; CCND1/IGHG1 fusion gene; CCND1/IGHG1 fusion gene; included; CCND1/IGLC1 fusion gene; CCND1/IGLC1 fusion gene; included; CCND1/PTH fusion gene; CCND1/PTH fusion gene; included; CCND1_HUMAN; cD1; Cyl 1; D11S287E; G1/S specific cyclin D1; G1/S-specific cyclin-D1; Parathyroid adenomatosis 1; PRAD1; PRAD1 oncogene; U21B31 |
| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 1-295aa |
| Target Protein Sequence | MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 37.7kDa |
| Protein Length | Full Length |
| Tag Info |
N-terminal 6xHis-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
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| Gene References into Functions |
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| Involvement in disease | Multiple myeloma (MM) |
| Subcellular Location | Nucleus. Cytoplasm. Nucleus membrane. |
| Protein Families | Cyclin family, Cyclin D subfamily |
| Database Links |
HGNC: 1582 OMIM: 168461 KEGG: hsa:595 STRING: 9606.ENSP00000227507 UniGene: Hs.523852 |
