CCR9 Antibody

Datasheet
Code CSB-RA004848A0HU
Size US$350
Uniprot No. P51686
Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue
    All lanes: CCR9 antibody at 1μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 43, 41 KDa
    Observed band size: 43 KDa

  • Immunofluorescence staining of HepG2 cells with CSB-RA004848A0HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitating CCR9 in HL-60 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA004848A0HU in HL-60 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA004848A0HU (3μg) + HL-60 whole cell lysate (500μg)
    Lane 3: HL-60 whole cell lysate (20μg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Immunogen A synthesized peptide derived from human CCR9
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IF, IP; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Relevance Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6, CDw199, CCR9, GPR28
Immunogen Species Human
Research Area Immunology
Gene Names CCR9
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Highly expressed in the thymus and low in lymph nodes and spleen.
Database Links

HGNC: 1610

OMIM: 604738

KEGG: hsa:10803

STRING: 9606.ENSP00000350256

UniGene: Hs.225946

Pathway Chemokine signaling pathway

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