Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue
All lanes: CCR9 antibody at 1μg/ml
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 41 KDa
Observed band size: 43 KDa
Immunofluorescence staining of HepG2 cells with CSB-RA004848A0HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating CCR9 in HL-60 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA004848A0HU in HL-60 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA004848A0HU (3μg) + HL-60 whole cell lysate (500μg)
Lane 3: HL-60 whole cell lysate (20μg)