Code | CSB-RA780821A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IP | 1:200-1:1000 |
The synthesized DNA sequence corresponding to the CD19 monoclonal antibody was cloned into the plasmid and then transfected into the cell line for expression. The product was purified through the affinity-chromatography method to obtain the CD19 recombinant monoclonal antibody. This CD19 recombinant antibody is a rabbit IgG and has been tested in scientific applications, including ELISA, WB, IHC, and IP. It only reacts with human CD19.
Sophia Roßkopf et al. revealed that the combination of Fc glyco-engineering and protein-engineering could potentiate ADCC and CDC in CD19 antibodies. Dorothee Winterberg et al. designed a fusion protein formed by fusing a monoclonal CD19-directed IgG antibody to scTRAIL and showed that the CD19-TRAIL efficiently killed CD19-positive BCP-ALL cell lines in vitro and in vivo and was also effective in BCP-ALL xenograft mouse models.
Applications : Western blot analysis
Sample type: cell
Review: The expression of hub proteins (TNF, ESR1, MCL1, TBP, CD19, LCK, PCNA, CHEK1, and POLA1) in HeLa cells treated with N-CM and H-CM. The expression of hub proteins in HeLa cells treated with N-CM and H-CM for 24 h was analyzed with Western blotting.
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