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To create the recombinant monoclonal antibody directed against CD19, the first step involves the incorporation of CD19 antibody genes into plasmid vectors. These modified plasmid vectors are then introduced into suitable host cells for expression using exogenous protein expression technology. Following this, the CD19 recombinant monoclonal antibody undergoes purification using affinity chromatography. It has undergone rigorous validation for specific applications, including ELISA and WB. It is essential to note that this antibody binds to both human and mouse CD19 proteins.
CD19 protein is a key co-receptor on the surface of B cells that plays a central role in B cell activation, differentiation, and regulation of the adaptive immune response. Its functions are critical for mounting effective immune responses to pathogens and maintaining immune system balance.
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