Code | CSB-RA081853A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
FC | 1:50-1:200 |
CUSABIO's approach to developing a recombinant monoclonal antibody against CREB1 commenced with the immunization of a rabbit using a synthesized peptide from human CREB1 protein. B cells were subsequently isolated from the immunized rabbit, and RNA was extracted from these B cells. The extracted RNA was reverse-transcribed into cDNA, which served as a template for extending CREB1 antibody genes using degenerate primers. The engineered CREB1 antibody genes were incorporated into a plasmid vector and transfected into host cells for expression. The resulting CREB1 recombinant monoclonal antibody was then purified from the cell culture supernatant using affinity chromatography. Its suitability for ELISA, WB, and FC applications was confirmed, demonstrating specific reactivity with CREB1 proteins from human, mouse, and rat species.
CREB1 is a crucial transcription factor that regulates gene expression in response to a wide range of signals and stimuli. Its roles extend to various physiological processes, including learning and memory, cell growth and differentiation, metabolism, and the cellular response to stress.
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