Code | CSB-RA005947A133phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The vectors expressing anti-CREB1 antibody were constructed as follows: immunizing an animal with a synthesized peptide derived from human Phospho-CREB1 (S133), isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening CREB1 antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant CREB1 antibody. Recombinant CREB1 antibody in the culture medium was purified using affinity-chromatography. It can react with CREB1 protein from Human and is used in the ELISA, WB, IHC, IF.
In adult mammalian retina, p-CREB1 is normally limited to the ganglion cell and inner nuclear layers. It appears that as in other parts of the nervous system, stressful stimuli can induce phosphorylation of CREB1 in retinal neurons. CREB1 not only controls the expression of its own direct target genes, but is also involved in signaling crosstalk with nuclear receptors such as the glucocorticoid receptor and ERα. Whether CREB1 stimulates or represses nuclear receptor activity seems to be cell-context dependent. Upon phosphorylation of serine 133 by PKA, pCREB1 can specifically recruit the coactivator CREB binding protein (CBP) and its paralog p300. The stimulatory activity of CREB1 requires its DNA binding and activation by phosphorylation, and affects the chromatin recruitment of ERα. CREB1 and ERα are biochemically associated and share hundreds to thousands of chromatin binding sites upon stimulation by estrogen and cAMP, respectively.
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