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The GPC3 recombinant monoclonal antibody was meticulously produced by CUSABIO using a systematic approach. Initially, isolating B cells from the spleen of an immunized animal. The recombinant human GPC3 protein was used as the immunogen. Following this, extracting RNA from the B cells and converting it into cDNA through reverse transcription. Using the cDNA as a template, the gene encoding the GPC3 antibody was amplified with a degenerate primer and inserted into a vector. This recombinant vector was then transfected into host cells, facilitating the expression of the GPC3 recombinant monoclonal antibodies. These antibodies were subsequently harvested from the cell culture supernatant and purified using affinity chromatography. It has been confirmed that this antibody can detect human GPC3 protein in ELISA.
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