Product Details

Uniprot No.

Target Names

Alternative Names

BA554C12.1 antibody; E3 ubiquitin-protein ligase RBX1 antibody; FLJ60363 antibody; MGC13357 antibody; MGC1481 antibody; OTTHUMP00000028983 antibody; Protein ZYP antibody; Rbx 1 antibody; Rbx1 antibody; RBX1_HUMAN antibody; Regulator of cullins 1 antibody; Ring box 1 antibody; Ring box 1 E3 ubiquitin protein ligase antibody; RING box protein 1 antibody; RING finger protein 75 antibody; RING finger protein antibody; RING-box protein 1 antibody; Ringbox protein 1 antibody; RNF 75 antibody; RNF75 antibody; ROC 1 antibody; ROC1 antibody; ZYP protein antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human RBX1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RBX1, also known as RING-box protein 1 or ROC1, serves as an essential component of the Cullin-RING E3 ubiquitin ligase complexes that regulate protein degradation through the ubiquitin-proteasome pathway. This small but critical protein functions as the catalytic subunit that recruits E2 ubiquitin-conjugating enzymes, making it indispensable for cellular processes including cell cycle progression, DNA damage response, and signal transduction. Given its involvement in regulating the stability of numerous substrates, RBX1 has emerged as a significant focus in cancer biology and therapeutic development research.

This recombinant monoclonal antibody, clone 7D3, offers researchers the reproducibility advantages inherent to recombinant technology. Generated against a synthetic peptide derived from human RBX1 and produced in rabbit host, the sequence-defined nature of this antibody ensures consistent performance across experiments and eliminates the lot-to-lot variability that can complicate long-term studies.

Validation across multiple experimental platforms demonstrates genuine workflow flexibility. Western blot analysis confirms specific detection of RBX1 at the expected 12 kDa molecular weight across a diverse panel of human cell lines including HEK293, HeLa, COLO205, HepG2, U-251MG, PC-3, K562, A549, and Jurkat cells, indicating robust performance across different cellular contexts. Immunohistochemistry validation in paraffin-embedded human colorectal cancer tissue demonstrates suitability for archival sample analysis. Immunofluorescence staining in PC-3 cells and flow cytometry analysis of Jurkat cells further extend the antibody's utility for subcellular localization studies and quantitative single-cell analysis.

This antibody supports investigations into ubiquitin-mediated proteolysis, Cullin-RING ligase biology, and the mechanistic underpinnings of diseases where protein homeostasis is disrupted.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Recruits the E2 ubiquitin-conjugating enzyme CDC34 to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Gene References into Functions

The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrate ribosome quality control of premature polyadenylated mRNAs. PMID: 28685749
SESN2 is critical to protect cells against detrimental effect of mitochondrial damage and RBX1 is a negative regulator of SESN2 which hampers its stabilization. PMID: 29294217
RBX-1 overexpression in bladder UCC is associated with high tumor grade and advanced stage and represents biological potential of invasiveness and aggressive disease. PMID: 28946546
The crystal structure of a pentameric CRL2(VHL) complex, composed of Cul2, Rbx1, Elongin B, Elongin C, and pVHL reveals how Rbx1 interacts with Cullin 2. PMID: 28591624
Study demonstrated that ROC1 was overexpressed in esophageal squamous cell carcinomas, which was positive associated with poor prognosis of esophageal cancer patients. PMID: 28418860
p97 negatively regulates NRF2 through the canonical pathway by extracting ubiquitylated NRF2 from the KEAP1-CUL3 E3 complex. PMID: 28115426
Regulation of substrate ubiquitination and polyubiquitination takes place through Rbx1 rotations. PMID: 28082425
High RBX1 expression was related to poor tumor differentiation, advanced TNM stage, and lymph node metastasis in non-small cell lung cancer. PMID: 27566015
Data show that melanoma antigen, family C, 2 protein (MAGE-C2) binds with RING-box protein 1 (Rbx1) and Cullin 1, and regulates cyclin E stability in melanoma cells. PMID: 26540345
ROC1 has an important role in the malignant progression of bladder transitional cell carcinoma via the mTOR/DEPTOR pathway. PMID: 26742010
Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8. PMID: 26632597
RBX1 E3 ubiquitin protein expression responsible with multiple genetic mechanism in the development of head and neck cancer. PMID: 24596130
This work sheds new light on the roles of NEDD8 lysines on neddylation cascades and provides a dominant negative mutant for the study of neddylation and its biological functions. PMID: 25918018
These findings indicate that Rbx1 and Rbx2 can both activate Cul5-Vif E3 ligase in vitro, but they may undergo a more delicate selection mechanism in vivo. PMID: 25912140
upregulation of miR-194 can inhibit proliferation, migration, and invasion of GC cells, possibly by targeting RBX1. Aberrant expression of miR-194 and RBX1 is correlated to GC patient survival time. PMID: 25412959
RBX1 expression level was associated with the proliferation of gastric cancer. PMID: 24292229
Studied the DNA-level mechanisms affecting KEAP1/CUL3/RBX1 E3-ubiquitin ligase complex as a regulator of NRF2 levels in ovarian cancer. PMID: 25114896
eEF1A1 may mediate SAMHD1 turnover by targeting it to the proteosome for degradation through association with Cullin4A and Rbx1. PMID: 25423367
data suggest a new paradigm for Hsp90-modulated assembly of a Cul3/DBC2 E3 ubiquitin ligase complex that may extend to other E3 ligase complexes. PMID: 24608665
CUL4A-DDB1-Rbx1 E3 ligase controls the quality of the PTS2 receptor Pex7p. PMID: 24989250
A genome-wide association study on a southern European population identified a new Crohn's disease susceptibility locus at RBX1-EP300. PMID: 22936669
Overexpression of RBX1 protein contributes to tumor progression and poor prognosis of non-muscle-invasive bladder cancer (NMIBC). PMID: 23609182
ROC1 knockdown significantly inhibited the growth of liver cancer cells by sequentially and independently inducing autophagy and p21-dependent cell senescence. PMID: 22935614
a model by which N-terminal cleavage of RBX1 impairs its activity and promotes susceptibility to ER stress induction. PMID: 22822056
Structural and biochemical analyses reveal that GLMN adopts a HEAT-like repeat fold that tightly binds the E2-interacting surface of RBX1, inhibiting CRL-mediated chain formation by the E2 CDC34. PMID: 22748924
Numb regulates glioma stem cell fate and growth by altering epidermal growth factor receptor and Skp1-Cullin-F-box ubiquitin ligase activity PMID: 22553175
Glomulin binds Rbx1 and regulates cullin-1 RING ligase-mediated turnover of Fbw7. PMID: 22405651
the SCF complex (Skp1/Cul1/F-box protein/Roc1) intervenes in the surveillance of Cdh1 cellular abundance in S-phase PMID: 16123585
Results indicate that FBW5-DDB1-CUL4-ROC1 is an E3 ubiquitin ligase regulating TSC2 protein stability and TSC complex turnover. PMID: 18381890
Data show that REDD1 is subject to ubiquitin-mediated degradation mediated by the CUL4A-DDB1-ROC1-beta-TRCP E3 ligase complex and through the activity of glycogen synthase kinase 3beta. PMID: 19557001
KLHL20-Cul3-ROC1 is an E3 ligase for DAPK ubiquitination PMID: 20389280
A new conformation of RBX1 involved in the e2-to-substrate ubiquitin-like protein transfer is discussed. PMID: 21765416
ROC1 expression is negatively correlated with cyclin D1 expression, demonstrating its importance in the degradation of cyclin D1 in melanomas. PMID: 21300445
in human cancer cells, RBX1 silencing causes the accumulation of DNA replication licensing proteins CDT1 and ORC1, leading to DNA double-strand breaks, DDR, G(2) arrest, and, eventually, aneuploidy PMID: 21115485
Data show that CRN7 interacted with Cullin1 and Roc1 to form a novel SCF-like E3 complex. PMID: 21130766
Nrf2 regulates Cul3-Rbx1 by controlling regulation of expression and induction of Cul3-Rbx1 PMID: 20452971
The data suggest that RBX1 could potentially be developed into biomarkers of resistance to acyl sulfonamide-based cancer drugs. This will require clinical validation in a series of patients treated with R3200. PMID: 19723642
crystal structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF complex PMID: 11961546
findings report that HIV-1 Vif interacts with cellular proteins Cul5, elongins B and C, and Rbx1 to form an Skp1-cullin-F-box (SCF)-like complex PMID: 14564014
DET1 promotes ubiquitination and degradation of c-Jun by assembling a multisubunit ubiquitin ligase containing DNA Damage Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1 (ROC1), and constitutively photomorphogenic-1 PMID: 14739464
Keap1 negatively regulates Nrf2 function in part by targeting Nrf2 for ubiquitination by the CUL3-ROC1 ligase and subsequent degradation by the proteasome PMID: 15601839
This study uncovers CUL4-DDB-ROC1 as a histone ubiquitin ligase and demonstrate that histone H3 and H4 ubiquitylation participates in the cellular response to DNA damage. PMID: 16678110
ROC1 protein binds to the proinactive form of pro-caspase 3 and shortens the half life of the enzyme. PMID: 17217622
VprBP depletion abolished the in vivo interaction of Merlin and Roc1-Cullin4A-DDB1, which resulted in Merlin stabilization and inhibited ERK and Rac activation PMID: 18332868
CUL1 ECTD (extreme C-terminal domain; spanning the C-terminal 50 amino acids), did not contribute to CUL1's stable association with ROC1. PMID: 18723677
SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation PMID: 18826954
ROC1 silencing triggers multiple death and growth arrest pathways to effectively suppress tumor cell growth, suggesting that ROC1 may serve as a potential anticancer target PMID: 19509229

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

RING-box family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 9928

OMIM: 603814

KEGG: hsa:9978

STRING: 9606.ENSP00000216225

UniGene: Hs.474949

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Code	CSB-RA157424A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate, HeLa whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HepG2 whole cell lysate, U-251MG whole cell lysate(30µg), PC-3 whole cell lysate, K562 whole cell lysate(30µg), A549 whole cell lysate, Jurkat whole cell lysate(30µg) All lanes: RBX1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 30s IHC image of CSB-RA157424A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of PC-3 cell with CSB-RA157424A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA157424A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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RBX1 Recombinant Monoclonal Antibody

Product Details

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Target Background