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The STAT3 recombinant monoclonal antibody was produced by first incorporating STAT3 antibody genes into plasmid vectors. These constructed plasmid vectors were then introduced into appropriate host cells to enable antibody expression using exogenous protein expression technology. Following this, the STAT3 recombinant monoclonal antibody underwent purification via affinity chromatography and was subsequently verified for its suitability in ELISA assays. In a functional ELISA test, it was determined that this STAT3 recombinant monoclonal antibody could efficiently bind to the human STAT3 protein (CSB-BP022812HU(A4)j7) at a concentration of 2 μg/mL, displaying an EC50 within the range of 41.31 to 75.59 ng/mL.
STAT3 is a multifunctional protein that plays a central role in cellular signaling, immune responses, inflammation, and various physiological and pathological processes. Its regulation of gene expression makes it a key player in coordinating cellular responses to extracellular signals and maintaining tissue homeostasis. Dysregulation of STAT3 signaling can have profound implications for health and disease.
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