Phospho-STAT3 (S727) Antibody

Datasheet
Code CSB-RA022812A727phHU
Size US$350
Uniprot No. P40763
Image
  • Western Blot
    Positive WB detected in:A549 whole cell lysate(treated with EGF or not)
    All lanes:Phospho-STAT3 antibody at 1.88μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 98 KDa
    Observed band size: 98 KDa

  • IHC image of CSB-RA022812A727phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin A
    Lane 1: Rabbit control IgG(1μg)instead of CSB-RA022812A727phHU in 293 whole cell lysate treated with Calyculin A. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA022812A727phHU(3μg)+ 293 whole cell lysate treated with Calyculin A(1mg)
    Lane 3: 293 whole cell lysate treated with Calyculin A (20μg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunogen A synthesized peptide derived from Human Phospho-STAT3 (S727)
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Relevance Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3
Immunogen Species Homo sapiens (Human)
Research Area Signal Transduction
Gene Names STAT3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors
Involvement in disease Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant (AD-HIES); Autoimmune disease, multisystem, infantile-onset, 1 (ADMIO1)
Subcellular Location Cytoplasm, Nucleus
Protein Families Transcription factor STAT family
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Database Links

HGNC: 11364

OMIM: 102582

KEGG: hsa:6774

STRING: 9606.ENSP00000264657

UniGene: Hs.463059

Pathway Chemokine signaling pathway
HIF-1 signaling pathway
Jak-STAT signaling pathway
Necroptosis
Signaling pathways regulating pluripotency of stem cells
Th17 cell differentiation
Adipocytokine signaling pathway
AGE-RAGE signaling pathway in diabetic complications
FoxO signaling pathway

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