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| Code | CSB-RA022812A727phHU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IHC | 1:50-1:200 |
| IP | 1:200-1:1000 |
The phospho-STAT3 (S727) recombinant monoclonal antibody is a highly specific antibody against the pS727-STAT3 from human sources. This phospho-STAT3 (S727) antibody was expressed by transfecting the human phospho-STAT3 (S727) monoclonal antibody gene-vector clones into the cell line for in vitro production and subsequent purification from the tissue culture supernatant (TCS) through affinity-chromatography. Its isotype matches with the rabbit IgG. This phospho-STAT3 (S727) antibody can be used in ELISA, WB, IHC, and IP applications.
STAT3 has a role in a variety of biological processes, including cancer and immunology. When JAKs associated with cytokine-stimulated receptors phosphorylate STAT3 monomers at tyrosine 705 (pY705), cytoplasmic STAT3 monomers dimerize and translocate to the nucleus, where the homodimers promote target gene transcription. Members of the MAPK and c-Jun N-terminal kinase families can also phosphorylate STAT3 at serine 727 (pS727). The role of pS727 has been shown to improve STAT3's transcriptional activity, and later pS727 was shown to reduce the duration of STAT3's transcriptional activity for the socs3 gene by promoting pY705 dephosphorylation.
Applications : Immunoblotting
Sample type: cells
Review: Cells were transduced with NEK2 oe or sh-NEK2#1/sh-NEK2#2 for 24 h and then treated with IL-22 (100 ng/mL) for 24 h and examined for the protein levels of p-STAT3 by Immunoblotting.
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