Code | CSB-RA546821A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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FC | 1:20-1:200 |
CUSABIO immunized a rabbit with a human STAT3-derived peptide and obtained splenocytes from this immunized rabbit. Extract RNA from the splenocytes and perform reverse transcription of the extracted RNA to convert it into cDNA. With the synthesized cDNA as the template, the STAT3 antibody gene will be extended with the degenerate primer. PCR amplified the STAT3 antibody gene fragments. The purified STAT3 antibody gene fragments were cloned into an expression vector and transfected into a host system for antibody expression. The STAT3 recombinant monoclonal antibodies were purified from the cell culture supernatant using affinity chromatography. The binding specificity and affinity of the recombinant STAT3 monoclonal antibody were confirmed using two applications ELISA and FC. This antibody specifically recognizes the human STAT3 protein.
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