Recombinant Human Interferon gamma protein(IFNG),(Active)

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Code CSB-AP002801HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml.
Target Names IFNG
Uniprot No. P01579
Research Area Immunology
Alternative Names IFN-gamma
Species Homo sapiens (Human)
Source E.coli
Expression Region M+24-166aa
Complete Sequence M+QDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ
Mol. Weight 16.9 kDa
Protein Length Partial
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Gene References into Functions
  1. Impaired IFNgamma-Signaling and Mycobacterial Clearance in IFNgammaR1-Deficient Human iPSC-Derived Macrophages. PMID: 29249666
  2. High expression of IFNG is associated with breast cancer. PMID: 30336781
  3. results suggest, the interplay of pro-inflammatory cytokines IFN-gamma derived from CD4+T lymphocytes and TNF-alpha from CD14+ cells has no direct additive impact on parasite replication but induces IL-4 production. PMID: 29953494
  4. IFN-lambda may participate in local inflammation in the salivary glands of primary Sjogren's syndrome patients through direct and indirect regulations of the expressions of BAFF and CXCL10 in salivary gland epithelium. PMID: 28421993
  5. The expression of CXCL10 mRNA and IFN-gamma mRNA was significantly higher in non-lesional and perilesional skin of vitiligo and alopecia areata patients compared with the skin of healthy controls; however, the level of expression of CXCL10 and IFN-gamma in lesional skin was not different than that in healthy skin PMID: 27863059
  6. High IFNG expression is associated with Chronic Periodontitis. PMID: 30051674
  7. hypomethylation of the IFNG promoter is significantly related to the risk of essential hypertension PMID: 29643275
  8. These results demonstrated that the IFNGinduced immunosuppressive properties of B7H1 in human BM and WJMSCs were mediated by STAT1 signaling, and not by PI3K/RACalpha serine/threonineprotein kinase signaling PMID: 29901104
  9. serum IP-10 level and the IFN-gamma/IL-4 ratio have great potential to predict significant fibrosis among chronic hepatitis B patients. PMID: 28067328
  10. IFN-gamma increases free ISG15 levels in the cytoplasm and ISGylation in the nucleus and cytoplasm, but in a manner distinct between MCF-7 and MDA-MB-231cells. PMID: 29626479
  11. the expression of IFN-gamma and IL-17 was also suppressed by IRAK1/4 inhibitor both in active Behcet's patients and in normal subjects. PMID: 28780618
  12. IFNgamma induces a rapid activation of aerobic glycolysis followed by a reduction in oxidative phosphorylation in M1 macrophages. PMID: 29463472
  13. Results provide evidence that rs2069707 locus SNPs of IFN-gamma is a risk factor for contracting tuberculous pericarditis. PMID: 30017738
  14. No correlation was observed between interferon gamma mRNA/protein levels and recurrent depressive disorders. PMID: 29367100
  15. Aberrant IFN-gamma promoter methylation may be involved in the process of tumorigenesis of oral cancer. PMID: 28091876
  16. this study shows that elevated levels of interferon-gamma are associated with high levels of Epstein-Barr virus reactivation in patients with the intestinal type of gastric cancer PMID: 29349089
  17. This study contributes to clarification of the previously inconsistent prognostic performance of IFNgamma by providing the first prognostic evaluation with long follow-up, time-dependence assessment and absence of any chemotherapy influence. PMID: 29478965
  18. Association Between the Interferon Gamma 874 T/A Polymorphism and the Severity of Valvular Damage in Patients with Rheumatic Heart Disease. PMID: 29332266
  19. IFN-gamma can promote cancer immunoevasion. (Review) PMID: 29283429
  20. an electrophoretic mobility shift assay showed that signal transducers and activators of transcription 1 (STAT1) attach to the GAS motif on the human STING promoter region. This indicates that IFN-gamma/Janus kinases/STAT1 signaling is essential for the STING upregulation in human keratinocytes. PMID: 29143896
  21. we review the direct and indirect effects of IFN-gamma on hematopoiesis, as well as the underlying signaling mechanisms of how IFN-gamma modulates the self-renewal, cell cycle entry, and proliferation of hematopoietic stem cells PMID: 28852997
  22. IFN-gamma +874T allele may increase the risk of ocular lesions in Toxoplasma infection. The principle of natural selection seems to also play a role. The less common TNF-308A allelic form could be protective against the development of Toxoplasma ocular infection. PMID: 27081842
  23. this study shows the age-related reductions in serum and PBMC IFN-gamma in healthy nonobese subjects PMID: 28762199
  24. Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
  25. SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID: 29180417
  26. the frequencies of IFNgamma and IL-17A(+) cells were increased in the antrum, particularly in patients with H. pylori induced gastric ulcers. PMID: 28683359
  27. rs2069718 in the IFNG gene was significantly associated with pulmonary tuberculosis but not spinal tuberculosis. PMID: 28867622
  28. IFN-gamma was associated with a cerebral volume reduction in systemic lupus erythematosus with central nervous system involvement PMID: 28848179
  29. Data suggest that semen exhibits substantial individual variation over time in pro-inflammatory seminal fluid cytokines IFNG and CXCL8. (IFNG = interferon gamma; CXCL8 = C-X-C motif chemokine ligand 8) PMID: 28541460
  30. Dysregulation of the IFN-gamma-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia PMID: 29474442
  31. STAT1b plays a key role in enhancing the tumor suppressor function of STAT1a, in ESCC, in a manner that can be amplified by IFN-gamma PMID: 28981100
  32. epigenetic silencing by single CpG methylation determines differential IL18BP inducibility in monocytic versus epithelial cells. T PMID: 29409936
  33. Systemic IFN activation is associated with higher activity only in the ESSDAI biological domain but not in other domains or the total score. Our data raise the possibility that the ESSDAI biological domain score may be a more sensitive endpoint for trials targeting either IFN pathway. PMID: 29474655
  34. These findings suggest that IFN-a can inhibit HCV replication through a STAT2-dependent but STAT1-independent pathway, whereas IFN-g induces ISG expression and inhibits HCV replication exclusively through a STAT1- and STAT2-dependent pathway. PMID: 27929099
  35. rs1861493 and rs2430561 polymorphisms were conformed to be in HWE in genotypes distribution of the control group (P>0.05 for both). However, only TT genotype and T allele of rs2430561 presented significantly higher frequencies in Ankylosing Spondylitis patients than in healthy controls. IFN-g rs2430561 polymorphism may contribute to the risk of IFN-g rs2430561 polymorphism may contributhrough influencing IFN-g expression. PMID: 28843049
  36. Interactions among polymorphisms of IFN-gamma+874 AA, IL-2-330 TT, IL-10-1082 AA, IL10--592 AC and IL-4-589 CC/CT significantly influenced the clinical progression of the subjects with hepatitis B virus and/or hepatitis C virus infection. PMID: 28838891
  37. Ex vivo interferon-gamma production is a useful biomarker for assessing disease activity and predicting poor clinical outcomes of systemic lupus erythematosus patients. PMID: 28841837
  38. this study demonstrates reduced IFN-gamma production in chronic brucellosis patients PMID: 28919584
  39. These data suggest that the de novo expression of PDL1 on tumor cells is upregulated by IFN-g secreted from CD8+ TILs upon recognition of the tumor cells with an MHC class I molecule. PMID: 28791392
  40. H pylori expression of cgt reduces cholesterol levels in infected gastric epithelial cells and thereby blocks IFNG signaling, allowing the bacteria to escape the host inflammatory response. PMID: 29273450
  41. Strategies to block MICA-NKG2D interactions resulted in reductions in IFNgamma production. Depletion of monocytes in vivo resulted in decreased IFNgamma production by murine NK cells upon exposure to Ab-coated tumor cells PMID: 28724544
  42. study concluded that the IFN-gamma (874A/T) polymorphism is associated with the susceptibility to oral lichen planus PMID: 27544215
  43. IFN gamma induced upregulation of BCL6 was dependent on the classical STAT1 signaling pathway, and affected both major BCL6 variants. Interestingly, although IFN alpha induced stronger STAT1 phosphorylation than IFN gamma, it only slightly upregulated BCL6 in multiple myeloma lines. PMID: 29510136
  44. IFN-gamma, CXCL16 and uPAR are promising as effective biomarkers of disease activity, renal damage, and the activity of pathological lesions in systemic lupus erythematosus. PMID: 28628472
  45. serum levels of soluble FAS ligand (sFASL) and interferon gamma (IFN-gamma) were analyzed and correlated with sFGL2 levels in Hepatitis C Virus-Infected Patients and Hepatocellular Carcinoma Patients. PMID: 28609212
  46. IFN-gamma induces activated but insufficient autophagy and thus contributes to a degree to p62-dependent apoptosis of nasal epithelial cells in chronic rhinosinusitis with nasal polyps. PMID: 28258963
  47. results suggested that IFN-gamma induces autophagy-associated apoptosis in CRC cells via inducing cPLA2-dependent mitochondrial ROS production. PMID: 29551681
  48. Activated interferon-gamma-producing CD56(bright) NK cells are positioned to play a key role in the fibrotic process and progression to chronic kidney disease. PMID: 28396119
  49. Posttransplant immune monitoring by donor-specific IFN-gamma ELISPOT can assess risk for developing subclinical T-cell mediated rejection and anti-donor HLA antibodies. PMID: 28274484
  50. genetic polymorphism is not associated with increased susceptibility to chronic spontaneous urticaria in Iran PMID: 28159384
  51. Chemokine profiling in addition to IFN-gamma can be used to differentiate among Mycobacterium tuberculosis infection possibilities. PMID: 28610785
  52. Aberrant Th1 immune responses in biliary atresia promote the proliferation and secretion of hepatic stellate cells through the IFN-gamma/STAT1 pathway. PMID: 28304404
  53. More prominent IL-17 and IFN-gamma production by all lymphocyte subsets in Behcet disease (BD) might be associated with the increased innate responses, early tissue neutrophil infiltrations and late adaptive immunity in BD. PMID: 28414558
  54. The effector memory T-cell compartment was the primary source of IFNgamma, and culture of T cells and these BiTEs with plate-bound rMICA showed ligand density-dependent production of IFNgamma from both CD4(+) and CD8(+) T cells. There was 2-fold more IFNgamma produced per CD8(+) T cell and 5-fold greater percentage of CD8(+) T cells producing IFNgamma compared with CD4(+) T cells. PMID: 28500232
  55. IFN-gamma +874 non-AA genotype is associated with more severe thrombocytopenia and a higher Th1/Th2 ratio in chronic immune thrombocytopenia , indicating that the cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic immune thrombocytopenia PMID: 28511637
  56. data suggest that IFN-gamma close to the dorsal nerve roots may contribute to the pathogenesis, the nociceptive activity and the pain behavior following lumbar disc herniation. PMID: 28651128
  57. In dermatomyositis muscle specimens, IL-4 and IFN-g levels were positively correlated, while no correlation was observed between IL-17 and the other two cytokines. Moreover, IL-4 and IFNg levels were significantly negative correlated with muscle strength grades for the deltoid muscle PMID: 28652434
  58. His-FLAG Tag as a Fusion Partner of Glycosylated Human Interferon-Gamma and Its Mutant. PMID: 28685146
  59. Data point out to a broad set of activities shared by IL-27 and IFN-gamma, which are dependent on the common activation of the STAT1 pathway. These data add further explanation to the anti-tumor activity of IL-27 and also to its dual role in immune regulation PMID: 27683036
  60. Polymorphism of the rs2430561 locus of the IFNG gene is associated with increased risk for preeclampsia. PMID: 28981943
  61. These findings indicate that viral replication and inflammation are linked through a common IFNgamma-like, STAT-dependent pathway and that HIV-1-induced STAT1 and STAT3 signaling are involved in both inflammation and HIV-1 replication. PMID: 28142265
  62. Increased levels of IL-6, IFN-gamma, and IL-10 might be useful as diagnostic biomarkers for gastric cancer; however, this needs to be confirmed with larger number of patients and with control groups other than blood donors, properly age paired. PMID: 28558708
  63. the cause of active TB in the patients seems to be due to the lack of effective IFNgamma function or the lack of effective signaling connection between IFNgamma and its receptor in presence of -56 C/T polymorphism in promoter region of IFNgammaR1 gene PMID: 27941164
  64. The interferon-gamma (+874T/A) TA genotype was significantly associated with susceptibility to tuberculosis in patients compared to controls (OR = 1.76; 95%CI = 0.84-3.71; p = 0.007), while the interferon-gamma (+874T/A) TT genotype (OR = 0.51; 95%CI = 0.19-1.36; p = 0.007) had protective effects against tuberculosis and was related to a low risk of tuberculosis development. PMID: 29372577
  65. IFN-gamma polymorphisms may be involved in the development and progression of IgA Nephropathy. PMID: 28391282
  66. Following activation with T-cell receptor and co-culture with various concentrations of chrysotile fibers using freshly isolated CD4+ surface CXCR3 positive and negative fractions, the intracellular expression of CXCR3, IFNgamma and IL-17 remained unchanged when co-cultured with chrysotile. PMID: 28498408
  67. In this study, we first found that sPD-L1 was increased in patients with chronic hepatitis C (CHC). Our results indicate that the level of serum sPD-L1 might be associated with the progression of CHC and the generation of hepatocellular carcinoma PMID: 28539815
  68. expression of IL-9, IFN-gamma and IL-22 was higher in CLP compared to that of OLP PMID: 27696572
  69. Evaluation of interferon-gamma release assays in extrasanguinous body fluids for diagnosing tuberculosis PMID: 29421440
  70. Results of this study suggest that certain SNPs of IL-2 and IFN-gamma genes have association with predisposition of individuals to recurrent aphthous stomatitis. PMID: 28922104
  71. Results showed that IFNG upregulated PD-L1 expression in gastric cancer cells mainly through JAK-STAT pathway. PMID: 29034543
  72. The findings suggest that not the IFN-gamma (+874) A/T but the IL-12 (-1188) A/C polymorphism is correlated with subacute sclerosing panencephalitis (SSPE), and having an AA genotype or A allele decreases the risk of developing SSPE by 2.06- and 1.65-fold, respectively. PMID: 27103395
  73. This review focuses on the immune modulatory functions of IFN-gamma in cancer. PMID: 27502919
  74. Cytokines (IFNg and IL-4) estimation was done in the blood and NPA samples of cases and blood samples of controls.The mean levels of interleukin-4 in controls (blood), and cases (NPA and blood) were 1280.77, 956.08 and 692.37 pg/ml, respectively (P<0.05). PMID: 28474631
  75. Severe gastroenteritis and acute pancreatitis following rotavirus infection in children: The age-related failure of IFN-gamma. PMID: 27163635
  76. Our observations suggested that IL-10 -592 A/C, -1082 G/A, and IFN-gamma +874 T/A polymorphisms had a strong association with susceptibility to HCV infection. However, no significant association was observed between the cytokines (IL-10 and IFN-gamma) genotypes profile and HCV-liver cirrhosis risk in the studied population, except for the high frequency of IFN-gamma +874 T allele in cirrhotic patients. PMID: 28387594
  77. Results showed that plasma and mRNA levels of IL-2, IFN-gamma, IL-4, and IL-17A in primary immune thrombocytopenia patients did not change significantly after TPO-RA treatment, whereas TGF-beta1 levels increased remarkably. PMID: 28142109
  78. These findings demonstrate how IFNgamma induced CK2 regulates RIG-I to drive a complex program of metabolic adaptation and redox homeostasis, crucial for determining glioma cell fate. PMID: 26631910
  79. Data indicate that dysregulated IFN-gamma secretion by NK cells contributed to a significant defect in STAT1 in patients with advanced melanoma in response to IL-2 stimulation. PMID: 27153543
  80. STAT4 mRNA expression was significantly correlated with IFNG mRNA expression. PMID: 29187449
  81. These studies suggest that innate cells by producing pro-inflammatory cytokines may contribute to pathogenesis. in patients with spondyloarthropathy. PMID: 28390966
  82. findings show HIV reservoir size is dependent on the presence of a defined NK cell population with a specific transcriptional signature: high NCR (NKp46 and NKp30) and IFN-gamma inducibility upon NCR and cytokine receptor engagement PMID: 28956765
  83. The increased expression of interferon gamma signaling and cholesterol biosynthesis pathways might explain part of the association between APOE4 and cardiovascular disease. Fish-oil supplementation may particularly benefit APOE4 carriers by decreasing expression of interferon gamma related genes. PMID: 27005961
  84. this study shows decreased expression of IFNG in schizophrenia and bipolar patients PMID: 29032575
  85. these results indicated association between splenomegaly, one of the most common complications in this disease, and IFN-g gene polymorphism PMID: 27288995
  86. crucial component of the regulatory network of joint capsule myofibroblasts PMID: 28176370
  87. The performance of IFNG release assays is robust despite variations in the incubation temperature between 37 degrees C and 39 degrees C for the diagnosis of latent tuberculosis infection. PMID: 27156612
  88. Elevated levels of systemic IFNgamma and IL-6 implied that the CEACAM5-specific T cells had undergone immune activation in vivo but only in patients receiving high-intensity pre-conditioning. PMID: 28660319
  89. Condensin II(CAP-D3 and CAP-H2) and GAIT subunits associate with L1 RNA in a co-dependent manner, independent of IFN-gamma. These findings suggest that cooperation between the Condensin II and GAIT complexes may facilitate a novel mechanism of L1 repression, thus contributing to the maintenance of genome stability in somatic cells PMID: 29028794
  90. The results highlight the importance of the inflammatory mediators, IFN-gamma and TLR4, in the pathogenesis of aseptic loosening; increased pro-inflammatory status was associated with early time to revision, whereas IL-4 correlated with longer implant survival PMID: 27669374
  91. Study provides evidence that mutations in IFNG confer melanoma cells resistance to ipilimumab. PMID: 27864228
  92. High IFNG expression is associated with susceptibility to HPV-16 positive cervical cancer. PMID: 27220278
  93. Graft dysfunction in chronic antibody-mediated rejection correlates with B-cell-dependent indirect antidonor alloresponses and autocrine regulation of interferon-gamma production by Th1 cells. PMID: 27988211
  94. Our results suggest that TNF-alpha, IFN-gamma and TGF-beta function cooperatively to regulate the expressions of IL-6 and MMP-9, and may play important roles in pathological behaviour of AMs, such as bone resorption. PMID: 27868311
  95. IFN-gamma is a promising immunological marker in vitiligo pathogenesis PMID: 28273427
  96. These data suggest that prior exposure to IFN-gamma may leave an epigenetic mark on the chromatin that enhances airway cells' ability to resist infection, possibly via epigenetic upregulation of RIG-I. PMID: 28481620
  97. Polymorphisms of IFNG, IL12B and IL12RB1 genes in paracoccidioidomycosis Brazilian patients do not affect the susceptibility or resistance to the disease. PMID: 27223631
  98. IL27 recapitulates IFN-gamma responses in hepatocellular carcinoma cells, but is inhibited by IL6-type cytokines. PMID: 27939431
  99. this study shows that genes associated with MAF-binding enhancers are suppressed in macrophages isolated from rheumatoid-arthritis patients, revealing a disease-associated signature of IFN-gamma-mediated repression PMID: 28813657
  100. This study presents preliminary evidence for the association between IFN +874 T/A, T/T, IL-10 -1082 A/G genotypes, and hemophagocytic lymphohistiocytosis susceptibility in Chinese children PMID: 28332776

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Involvement in disease Aplastic anemia (AA)
Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

HGNC: 5438

OMIM: 147570

KEGG: hsa:3458

STRING: 9606.ENSP00000229135

UniGene: Hs.856

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