Calpain-2 catalytic subunit is a protein in humans that is encoded by CAPN2 gene. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'.
The following CAPN2 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CAPN2 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004496LA01HU | CAPN2 Antibody |
Human | ELISA, WB, IHC, IF, IP |
CSB-PA004496GA01HU | CAPN2 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA060228 | CAPN2 Antibody |
Human,Mouse,Rat | IHC, ELISA |
CSB-PA257142 | CAPN2 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA247305 | CAPN2 Antibody |
Human,Mouse,Rat | ELISA,WB |
CAPN2 Antibodies for Pig
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004496LA01PI | CAPN2 Antibody |
Pig | ELISA |
CAPN2 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP004496HU CSB-BP004496HU CSB-MP004496HU CSB-EP004496HU-B |
Recombinant Human Calpain-2 catalytic subunit(CAPN2) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP004496HU | Recombinant Human Calpain-2 catalytic subunit(CAPN2) |
E.coli |
CAPN2 Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP004496PI CSB-BP004496PI CSB-MP004496PI CSB-EP004496PI-B |
Recombinant Pig Calpain-2 catalytic subunit(CAPN2) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP004496PI | Recombinant Pig Calpain-2 catalytic subunit(CAPN2) |
E.coli |
CAPN2 Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-YP004496RB CSB-EP004496RB CSB-BP004496RB CSB-MP004496RB CSB-EP004496RB-B |
Recombinant Rabbit Calpain-2 catalytic subunit(CAPN2) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CAPN2 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP004496MO CSB-EP004496MO CSB-BP004496MO CSB-MP004496MO CSB-EP004496MO-B |
Recombinant Mouse Calpain-2 catalytic subunit(Capn2) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CAPN2 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP631683BO CSB-EP631683BO CSB-BP631683BO CSB-MP631683BO CSB-EP631683BO-B |
Recombinant Bovine Calpain-2 catalytic subunit(CAPN2) ,partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CAPN2 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP849716CH CSB-EP849716CH CSB-BP849716CH CSB-MP849716CH CSB-EP849716CH-B |
Recombinant Chicken Calpain-2 catalytic subunit(CAPN2) ,partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CAPN2 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP867083MOV CSB-EP867083MOV CSB-BP867083MOV CSB-MP867083MOV CSB-EP867083MOV-B |
Recombinant Macaca fascicularis Calpain-2 catalytic subunit(CAPN2) ,partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CAPN2 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E17822h | Human calpain 2, (m/II) large subunit (CAPN2) ELISA kit |
serum, plasma, cell lysates | 0.039 ng/mL |
The CAPN2 encodes calpain-2, one type of isozymic calcium-dependent protease calpain that belongs to the cysteine protease family [1]. Calpain is ubiquitously expressed in the central nervous system CNS) of mammalian cells. Calpain exists as an inactive proenzyme in the cytosol and migrates to membranes in response to the increase of cellular calcium ion concentration [2]. At the membrane, Calpain is activated in the presence of calcium ion and phospholipids [3]. Calpain-2 is activated by millimolar concentrations of calcium. Inactive calpain-2 is associated with the ER (endoplasmic reticulum) membrane at the cytosolic side. Upon activation, calpain-2 is released and rapidly degraded. Opposite to the neuroprotective role of calpain-1, calpain-2 is neurodegenerative. Chang-jiang Huang et al. demonstrated that either increased cytosolic calcium ion concentration or hyperactivation of calpain-2 mediates beta-cell dysfunction and apoptosis in type 2 diabetes mellitus (T2DM) [4]. Experimental and clinical evidence indicates that CAPN2 is aberrantly expressed in various tumors. It plays a crucial role in tumorigenesis, disease progression, and therapy resistance [5][6][7]. Extrasynaptic Nmethyl- D-asparate receptors (NMDARs) specifically activate calpain-2, which degrades STEP, ultimately resulting in neurotoxicity [8].
[1] Ohno S, Minoshima S, et al. Four genes for the calpain family locate on four distinct human chromosomes [J]. Cytogenet Cell Genet. 1990, 53 (4): 225-9.
[2] Wingrave JM, Schaecher KE, et al. Early induction of secondary injury factors causing activation of calpain and mitochondria-mediated neuronal apoptosis following spinal cord injury in rats [J]. J Neurosci Res. 2003 Jul 1; 73(1):95-104.
[3] Suzuki K, Sorimachi H. A novel aspect of calpain activation [J]. FEBS Lett. 1998 Aug 14; 433(1-2):1-4.
[4] Chang-jiang Huang, Tatyana Gurlo, et al. Calcium-activated Calpain-2 Is a Mediator of Beta Cell Dysfunction and Apoptosis in Type 2 Diabetes [J]. The Journal of Biological Chemistry, 2010, 285, 339-348.
[5] Ho WC, Pikor L, et al. Calpain 2 regulates Akt-FoxO-p27(Kip1) protein signaling pathway in mammary carcinoma [J]. J Biol Chem. 287:15458-15465. 2012.
[6] Li P, Miao C, Liang C, et al. Silencing CAPN2 expression inhibited castration-resistant prostate cancer cells proliferation and invasion via AKT/mTOR signal pathway [J]. Biomed Res Int.
2017:25936742017.
[7] Liu B, Zhou Y, et al. Comparison of the protein expression of calpain-1, calpain-2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa [J]. Oncol Lett. 14:3705-3710.2017.
[8] Xu J, et al. Extrasynaptic NMDA receptors couple preferentially to excitotoxicity via calpain-mediated cleavage of STEP [J]. J Neurosci. 2009;29(29):9330-43.