UPF1 is a protein in humans that is encoded by UPF1 gene. RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.
The following Upf1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Upf1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA040201 | UPF1 Antibody |
Human,Mouse | WB, IHC, ELISA |
CSB-PA245266 | UPF1 Antibody |
Human,Mouse | ELISA,WB,IHC |
CSB-PA838819LA01HU | UPF1 Antibody |
Human | ELISA, IF |
CSB-PA838819LB01HU | UPF1 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA838819LC01HU | UPF1 Antibody, FITC conjugated |
Human | |
CSB-PA838819LD01HU | UPF1 Antibody, Biotin conjugated |
Human | ELISA |
Upf1 Antibodies for Arabidopsis thaliana (Mouse-ear cress)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA875473XA01DOA | UPF1 Antibody |
Arabidopsis thaliana | ELISA, WB (ensure identification of antigen) |
Upf1 Antibodies for Drosophila melanogaster (Fruit fly)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA896169XA01DLU | Upf1 Antibody |
Drosophila melanogaster (Fruit fly) | ELISA, WB (ensure identification of antigen) |
Upf1 Antibodies for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA283931XA01SXV | upf1 Antibody |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ELISA, WB (ensure identification of antigen) |
Upf1 Proteins for Dictyostelium discoideum (Slime mold)
Code | Product Name | Source |
---|---|---|
CSB-YP681606DKK CSB-EP681606DKK CSB-BP681606DKK CSB-MP681606DKK CSB-EP681606DKK-B |
Recombinant Dictyostelium discoideum Regulator of nonsense transcripts 1 (upf1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Upf1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP838819HU CSB-EP838819HU CSB-BP838819HU CSB-MP838819HU CSB-EP838819HU-B |
Recombinant Human Regulator of nonsense transcripts 1 (UPF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Upf1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP872108MO CSB-EP872108MO CSB-BP872108MO CSB-MP872108MO CSB-EP872108MO-B |
Recombinant Mouse Regulator of nonsense transcripts 1 (Upf1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Upf1 Proteins for Arabidopsis thaliana (Mouse-ear cress)
Code | Product Name | Source |
---|---|---|
CSB-YP875473DOA CSB-EP875473DOA CSB-BP875473DOA CSB-MP875473DOA CSB-EP875473DOA-B |
Recombinant Arabidopsis thaliana Regulator of nonsense transcripts 1 homolog (UPF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Upf1 Proteins for Drosophila melanogaster (Fruit fly)
Code | Product Name | Source |
---|---|---|
CSB-YP896169DLU CSB-EP896169DLU CSB-BP896169DLU CSB-MP896169DLU CSB-EP896169DLU-B |
Recombinant Drosophila melanogaster Regulator of nonsense transcripts 1 homolog (Upf1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |