Target Name
tumor necrosis factor (TNF superfamily, member 2)
Alternative Names
TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:1 | Average % | 95 | |
Range % | 89-105 | |
1:2 | Average % | 93 | |
Range % | 86-99 | |
1:4 | Average % | 97 | |
Range % | 90-105 | |
1:8 | Average % | 95 | |
Range % | 90-100 | |
Recovery
The recovery of dog TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 97 | 90-105 | |
EDTA plasma (n=4) | 95 | 93-99 | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
ng/ml | OD1 | OD2 | Average | Corrected | | 10 | 2.211 | 2.235 | 2.223 | 2.078 | | 5 | 1.672 | 1.684 | 1.678 | 1.533 | | 2.5 | 1.112 | 1.135 | 1.124 | 0.979 | | 1.25 | 0.629 | 0.639 | 0.634 | 0.489 | | 0.625 | 0.373 | 0.384 | 0.379 | 0.234 | | 0.312 | 0.264 | 0.277 | 0.271 | 0.126 | | 0.156 | 0.197 | 0.201 | 0.199 | 0.054 | | 0 | 0.144 | 0.145 | 0.145 | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Dog TNF ELISA Kit was designed for the quantitative measurement of Dog TNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .