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Human Interleukin 3,IL-3 ELISA KIT

Human Interleukin 3,IL-3 ELISA KIT

Specification
Target

Code

CSB-E04631h

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P08700

Lead Time

3-5 working days

Abbreviation

IL3

Target Name

interleukin 3 (colony-stimulating factor, multiple)

Alias

IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF, P-cell stimulating factor|hematopoietic growth factor|interleukin 3|mast-cell growth factor|multilineage-colony-stimulating factor

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This protein is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

HGNC

2436

RGD

620189

MGI

1339759

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-98
1:2	Average %	103
	Range %	91-108
1:4	Average %	92
	Range %	87-95
1:8	Average %	98
	Range %	90-101

Recovery

The recovery of human IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	91-106
EDTA plasma (n=4)	100	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.550	2.467	2.509	2.355
100	1.953	1.889	1.921	1.767
50	1.305	1.343	1.324	1.170
25	0.876	0.871	0.874	0.720
12.5	0.494	0.483	0.489	0.335
6.25	0.345	0.339	0.342	0.188
3.12	0.248	0.242	0.245	0.091
0	0.152	0.156	0.154

References

Elderly-onset hereditary pulmonary alveolar proteinosis and its cytokine profile Masayuki Ito.et al,BMC Pulmonary Medicine,Feb 17

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Function	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; FUNCTION
Subcellular Location	Secreted
Protein Families	IL-3 family
Tissue Specificity	Activated T-cells, mast cells, natural killer cells.
Database Links	HGNC: 6011 OMIM: 147740 KEGG: hsa:3562 STRING: 9606.ENSP00000296870 UniGene: Hs.694
Pathway	Jak-STAT signaling pathway PI3K-Akt signaling pathway Apoptosis Fc epsilon RI signaling pathway Hematopoietic cell lineage

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CSB-AP004521HU	Recombinant Human Interleukin-3(IL3)	Mammalian cell
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CSB-EP011652HU	Recombinant Human Interleukin-3(IL3)	E.coli
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Human Interleukin 3,IL-3 ELISA KIT

Specification

Target

Related Products

IL3 Antibodies

IL3 Proteins

Most popular with customers

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