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Human Interleukin 3,IL-3 ELISA KIT

Code CSB-E04631h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08700
Lead Time 3-5 working days
Abbreviation IL3
Target Name interleukin 3 (colony-stimulating factor, multiple)
Alias IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF, P-cell stimulating factor|hematopoietic growth factor|interleukin 3|mast-cell growth factor|multilineage-colony-stimulating factor
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
 Instructions
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
HGNC 2436
RGD 620189
MGI 1339759
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 94    
Range % 85-98    
1:2 Average % 103    
Range % 91-108    
1:4 Average % 92    
Range % 87-95    
1:8 Average % 98    
Range % 90-101    
Recovery
The recovery of human IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 101 91-106    
EDTA plasma (n=4) 100 92-104    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
200 2.550 2.467 2.509 2.355    
100 1.953 1.889 1.921 1.767    
50 1.305 1.343 1.324 1.170    
25 0.876 0.871 0.874 0.720    
12.5 0.494 0.483 0.489 0.335    
6.25 0.345 0.339 0.342 0.188    
3.12 0.248 0.242 0.245 0.091    
0 0.152 0.156 0.154 　    
References
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Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; FUNCTION
Subcellular Location Secreted
Protein Families IL-3 family
Tissue Specificity Activated T-cells, mast cells, natural killer cells.
Database Links

HGNC: 6011

OMIM: 147740

KEGG: hsa:3562

STRING: 9606.ENSP00000296870

UniGene: Hs.694
Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway
Apoptosis
Fc epsilon RI signaling pathway
Hematopoietic cell lineage

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