Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Instructions
Code CSB-E04756m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name vascular endothelial growth factor A
Alternative Names RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
Abbreviation VEGFA
Uniprot No. Q00731
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.906 pg/mL-250 pg/mL
Sensitivity 0.857 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Mouse VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development
Subcellular Location Isoform VEGF-1: Secreted, SUBCELLULAR LOCATION: Isoform VEGF-2: Secreted, SUBCELLULAR LOCATION: Isoform VEGF-3: Cell membrane, Peripheral membrane protein
Protein Families PDGF/VEGF growth factor family
Tissue Specificity In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in ki
Database Links

KEGG: mmu:22339

STRING: 10090.ENSMUSP00000115883

UniGene: Mm.282184

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