The mouse VEGFA ELISA Kit quantitates mouse VEGFA levels in multiple samples, including serum, plasma, cell culture supernates, and tissue homogenates. VEGFA usually referred to simply as VEGF, is a renowned angiogenic factor with potent mitogenic, pro-survival, anti-apoptotic, and vascular permeative properties. It plays an essential role in capillary maintenance, endothelial cell and myofiber survival, exercise-induced angiogenesis, and muscular endurance. VEGF binds to its receptor VEGFR1 and VEGFR2, activating several downstream signaling pathways, including MAPK and PI3K, that stimulate the expression of genes involving the promotion of endothelial cells' proliferation, migration, survival, and vascular permeability. VEGF-mediated signaling is associated with key aspects of tumorigenesis, including the function of cancer stem cells and tumor initiation.
This kit employs the sandwich-ELISA mechanism in conjugation with VEGFA antibody-VEGFA antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of VEGFA in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.