Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Code CSB-E04756m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name vascular endothelial growth factor A
Alternative Names Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
Abbreviation VEGFA
Uniprot No. Q00731
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.906 pg/mL-250 pg/mL
Sensitivity 0.857 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.


To assess the linearity of the assay, samples were spiked with high concentrations of Mouse VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Target Data

Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development
Gene References into Functions
  1. Study using Hcar1-KO mice identified the lactate receptor Hcar1 as a key regulator of Vegf and angiogenesis in the brain and as an initial mediator of cerebral effects of physical exercise. PMID: 28534495
  2. Motor neurons control blood vessel patterning by an autocrine mechanism that titrates motor neuron-derived VEGF via their own expression of sFlt1. PMID: 28262664
  3. Targeting NLRP3 shifts the VEGF-A-induced cardiac hypertrophy from a pathologic toward a more physiologic hypertrophy. PMID: 29146733
  4. T3 thyroid hormone stimulates the expression and secretion of VEGF by Leydig cells PMID: 29417848
  5. Dexamethasone suppressed mRNA VEGF expression and VEGF production in cortical cells while in medullar cells only VEGF production was reduced. Introduction of IL-7, IL-1b or murine thymocytes increased while addition of Semaphorin 3A, SDF-1a or ACTH decreased VEGF production by cortical epithelial cells with no influence on medullar cells. PMID: 30192114
  6. lack of endogenous PTH may reduce VEGF expression in bone marrow mesenchymal stem cellsderived osteoblasts. PMID: 29620150
  7. Upregulation of podocyte VEGF decreased the number of mesangial cells via inhibition of PDGF-B-mediated signaling. PMID: 28776225
  8. These data provide a new pathological perspective on cerebellar astrogliosis in Niemann-Pick type C disease and suggest the importance of VEGF as a therapeutic target for this disease. PMID: 29397865
  9. leukocyte domiciled midkine mediates increased plasma levels of VEGFA relevant for upregulation of endothelial nitric oxide synthase 1 and 3 PMID: 29233575
  10. Mesenchymal stem cells secrete VEGF which in turn mediates the differentiation of endothelial progenitor cells into endothelial cells. PMID: 29138837
  11. This study showed that the quantity of VEGF in the glioma microenvironment seems to be crucial for the participation of microglia/macrophages on tumor progression. PMID: 28948650
  12. AK131850 directly competed miR-93-5p in N-OC and M-OC through sponge, thereby increasing VEGFa transcription, expression and secretion through derepressing of miR-93-5p on VEGFa. PMID: 29590659
  13. miR203 expression may be upregulated by IL17 stimulation, and miR203 is a positive regulator of IL17induced VEGF secretion. PMID: 29039484
  14. NF-kappaBmiR15abFGF/VEGFA axis contributes to the impaired angiogenic capacity of bone marrowmesenchymal stem cells in high fat dietfed mice. PMID: 28944834
  15. Results support the idea that excess heparin binding epidermal growth factor-like growth factor (HB-EGF) leads to a significant elevation of vascular endothelial growth factor (VEGF) and ventricular dilatation. These data suggest a potential pathophysiological mechanism that elevated HB-EGF can elicit VEGF induction and hydrocephalus. PMID: 27243144
  16. Over-expression of VEGF-A165b is protective against proteinuria in a mouse model with progressive depletion of all endogenous VEGF-A splice isoforms from the kidney. PMID: 28574576
  17. The present data suggest that Ischemic preconditioning transiently increases plasma VEGF levels by downregulating miR-762 and miR-3072-5p in CD34-positive BM cells, leading to protection against organ ischemia. PMID: 27905554
  18. findings are the first to demonstrate the importance of CdGAP in embryonic vascular development and VEGF-induced signaling, and highlight CdGAP as a potential therapeutic target to treat pathological angiogenesis and vascular dysfunction PMID: 27270835
  19. VEGF165 induces differentiation of hair follicle stem cells into endothelial cells and plays a role in in vivo angiogenesis. PMID: 28244687
  20. TGF-beta1/TbetaRII/Smad3 signaling pathway increased VEGF expression in in oral squamous cell carcinoma tumor-associated macrophages (TAMs). TAMs can promote the tumor angiogenesis by secreting VEGF. PMID: 29462614
  21. VEGF causes extensive neural stem cell (NSC) remodelling manifested in transition of the enigmatic NSC terminal arbor onto long cytoplasmic processes engaging with and spreading over even remote blood vessels, a configuration reminiscent of early postnatal "juvenile" NSCs. PMID: 27849577
  22. effect of dox on VEGF-A levels might at least partly explain its previously reported beneficial effects on myocardial and brain ischemia. Also, this effect on VEGF-A should be taken into account in all studies using dox-regulated vectors PMID: 29351307
  23. Genetic depletion experiments revealed that VEGFR2, but not VEGFR3, is indispensable for maintenance of thyroid vascular integrity. Notably, blockade of VEGF-A or VEGFR2 not only abrogated vascular remodeling but also inhibited follicular hypertrophy, which led to the reduction of thyroid weights during goitrogenesis. PMID: 28438786
  24. Findings suggest that VEGF gene expression can be suppressed by TNFSF15-stimulated activation of the JNK-GATA3 signaling pathway which gives rise to up-regulation of miR-29b. PMID: 27589684
  25. The low-molecular-weight heparin (LMWH) Tinzaparin inhibited Von Willebrand factor (VWF) fiber formation and vessel occlusion in tumor vessels by blocking thrombin-induced endothelial cells (ECs) activation and vascular endothelial growth factor-A (VEGF-A)-mediated VWF release. PMID: 27602496
  26. These data suggest that VEGF expressed by skeletal myofibers may directly or indirectly regulate both hippocampal blood flow and neurogenesis. PMID: 28597506
  27. Results show that apoE4-driven brain pathology and cognitive impairments in young apoE4 TR mice are associated with down regulation of the VEGF system and can be reversed by upregulation of the expression of VEGF in the hippocampus. These animal model findings suggest that the VEGF system is a promising target for the treatment of apoE4 carriers in Alzheimers disease. PMID: 27372644
  28. It was shown that peritoneal macrophages are the main suppliers of VEGF at tumor angiogenesis, as evidenced by the data obtained on model system of endothelial cells synchronized in G0/G1 phase. PMID: 29235752
  29. these results uncover a novel role for VEGF in controlling proper allocation of Isl1(+) cardiac progenitors to their respective descending lineages PMID: 27794491
  30. endothelial master transcription factor ETS1 promotes global RNAPII pause release, and that this process is governed by VEGF PMID: 28851877
  31. Results provide evidence that VEGF derived from Osx+ osteoblast progenitor cells is required for optimal ossification of developing mandibular bones and modulates mechanisms controlling BMP-dependent specification and expansion of the jaw mesenchyme. PMID: 26899202
  32. The MDA-induced VEGF increase was inhibited by autophagy-lysosomal inhibitors. Intravitreal MDA injection in mice increased laser-induced choroidal neovascularization (laser-CNV) volumes. In a mouse model fed a high-linoleic acid diet for 3 months, we found a significant increase in MDA levels, autophagic activity, and laser-CNV volumes PMID: 26923802
  33. deletion of AT2 receptor reduced SHP-1 activity and restored VEGF actions, leading to an increased blood flow reperfusion after ischemia in diabetes mellitus. PMID: 29074590
  34. ata indicate that the HIF-1alpha/VEGF-A axis is an essential aspect of tumor immunity. PMID: 29136509
  35. It has been concluded that stimulation of VEGF release is a key factor in the promotion of macrophage proliferation by ceramide 1-phosphate. PMID: 29080796
  36. 8-Br-cAMP-induced cell-secreted VEGF is biologically active and may promote angiogenesis PMID: 24493289
  37. results suggested that the combination of nCS (to support bone formation) with a fibrin-based VEGF/FGF9 release system (support vascular formation) is an innovative and effective strategy that significantly enhanced ectopic bone formation in vivo. PMID: 27269204
  38. Astrocyte-derived vascular endothelial growth factor-A (VEGF-A) is known to induce BBB dysfunction.(review) PMID: 28966265
  39. CRP can upregulate vascular endothelial growth factor-A (VEGF-A) expression by activating hypoxia inducible factor-1alpha (HIF-1alpha) in ADSCs. PMID: 27526687
  40. VEGF and IGF1 were critical factors for the spontaneous cardiac differentiation of BATDCs, and MEK/ERK signaling was involved in the role of VEGF and IGF1. PMID: 27870972
  41. Distal retinal ganglion cell axon transport loss and activation of p38 MAPK stress pathway following VEGF-A antagonism have been documented. PMID: 27148685
  42. The neuroprotection observed in ColXV KO mice may be attributed to the increased VEGF-A production following stroke in the ischemic territory. PMID: 28079884
  43. VEGF protein levels were also higher in the ipsilateral hemisphere of WT mice compared to Par-1 KO mice after glioma cell implantation. PMID: 26463974
  44. the importance of VEGF derived from tumor-infiltrating myeloid cells for initiating vascularization in gliomas PMID: 26951383
  45. Low VEGF expression is associated with liver fibrosis. PMID: 28118605
  46. We conclude that HIF-1 is not a major regulator of Vegfa expression during wound healing; rather, it serves to maintain basal levels of expression of Vegfa and its target genes in intact skin, which are required for optimal granulation tissue formation in response to wounding. PMID: 28686658
  47. Mechanical strain stimulates vasculogenesis of embryonic stem cells by the intracellular messengers ROS, NO and calcium as well as by upregulation of angiogenesis guidance molecules and the angiogenic growth factors VEGF, FGF-2 and PDGF-BB. PMID: 27725190
  48. This study identifies YAP/TAZ as central mediators of VEGF signaling PMID: 28867486
  49. Ectopic midline vascularisation in endothelial Nrp1 and Vegfa(188/188) mutants caused additional axonal exclusion zones within the chiasm. PMID: 28676569
  50. VEGF inhibition decreases local CFH and other complement regulators in the eye and kidney through reduced VEGFR2/PKC-alpha/CREB signaling. PMID: 27918307
  51. Vascular endothelial growth factor (VEFG)-A, originally described as an angiogenic factor, belongs to a super-family of glycoproteins, and signals through tyrosine kinase receptors VEGF receptor 1 (VEGFR1) and VEGF receptor 2 (VEGFR2) and coreceptors, neuropilin (NP) 1 and 2. PMID: 28661183
  52. VEGF-A overexpression in adipocytes triggers angiogenesis. There was a rapid appearance of beige fat cells in subcutaneous white adipose tissue as early as 2 days postinduction of VEGF-A. In contrast to conventional cold-induced beiging, VEGF-A-induced beiging is independent of interleukin-4. PMID: 28254844
  53. Data show that Leishmania major infection initiates enhanced vascular endothelial growth factor-A/VEGFR-2 signaling and suggest that VEGFR-2-dependent lymphangiogenesis is a mechanism that restricts tissue inflammation in leishmaniasis. PMID: 27474074
  54. VEGFR3 limits VEGFR2 expression and VEGF/VEGFR2 pathway activity in quiescent and angiogenic blood vascular endothelial cells, thereby preventing excessive vascular permeability. PMID: 28298294
  55. VEGF165-induced vascular leakage requires both VEGFR2 and NRP1, including the VEGF164-binding site of NRP1 and the NRP1 cytoplasmic domain (NCD), but not the known NCD interactor GIPC1. PMID: 28289053
  56. Apelin could effectively promote mesenchymal stem cell survival and vascularization under hypoxic-ischemic condition in vitro, and this procedure was associated with the upregulation of VEGF. PMID: 28161441
  57. BLT2 expression in mast cells is essential for the production of VEGF in OVA-induced allergic asthma. PMID: 27489284
  58. Stability and species specificity of renal VEGF-A splicing patterns in kidney disease in humans and mice has been demonstrated. PMID: 27598902
  59. Novel VF-Trap fusion protein on blockage of VEGF and FGF-2 activity to prevent angiogenesis. PMID: 27130666
  60. E2F1 hinders skin wound healing by suppressing VEGF expression, neovascularization, and macrophage recruitment. Strategies that target E2F1 may enhance wound healing. PMID: 27490344
  61. The results demonstrated that long-term hyperglycaemia in conjunction with VEGF-driven retinal neovascularization increased Edn2 serum concentration suggesting Edn2 might be a candidate biomarker for vascular changes in diabetic retinopathy. PMID: 27482904
  62. Betaine has an anti-angiogenic effect on pathologic retinal neovascularization via suppression of ROS mediated VEGF signaling. PMID: 27473515
  63. p-mTOR, p-4EBP1, HIF-1alpha and VEGF together are involved in the pathogenesis of asthma. PMID: 28100332
  64. Renin-angiotensin system transgenic mouse model suggests that renal injury in preeclampsia may be mediated through local VEGF. PMID: 27927648
  65. findings demonstrated that a multi-component Chinese medicine DHI effectively increased blood flow recovery after tissue ischemia in diabetic mice by promoting angiogenesis and improving glucose tolerance through a concomitant activation of VEGF-A/VEGFR-2 and PPARdelta signaling pathways PMID: 27930695
  66. MicroRNA-29b inhibits angiogenesis by targeting VEGFA through the MAPK/ERK and PI3K/Akt signaling pathways in endometrial carcinoma. PMID: 28222438
  67. Data show that receptor for activated C-kinase 1 (RACK1) and vascular endothelial growth factor (VEGF) expression is up-regulated after choroidal neovascularization (CNV) formation. PMID: 27112838
  68. beta2-AR activation also stimulated VEGF and IL-6 mRNA expression by 2- and 10-fold, respectively. PMID: 28114591
  69. XBP1 regulates VEGF-mediated cardiac angiogenesis. PMID: 27133203
  70. First-in-class selective PET tracers for imaging VEGFR-1 and VEGFR-2 were constructed and successfully validated in an orthotopic murine tumor model. PMID: 27390161
  71. data for the first time demonstrate a calpain/PTP1B/VEGFR2 negative feedback loop in the regulation of VEGF-induced angiogenesis. Modulation of local PTP1B and/or calpain activities may prove beneficial in the treatment of impaired wound healing in diabetes. PMID: 27872190
  72. Furthermore, the combination of islet transplantation and atRA administration significantly rescued hyperglycemia in diabetic mice. These findings suggest that vitamin A derivatives can potentially be used as a supplementary treatment to improve diabetes management and glycemic control. PMID: 27381866
  73. The results demonstrate that the Vegf-Dll4/Notch feedback system normally operates to generate heterogeneity between endothelial cells driving blood vessel branching, whilst synchronization drives vessel expansion. PMID: 27074663
  74. Ginkgo biloba exocarp extract inhibits tumor angiogenesis, which may be closely relevant to its effect in blockage of Wnt /beta-catenin-VEGF signaling pathway in Lewis lung carcinoma. PMID: 27649680
  75. D2R agonist treatment blocks tumor growth, induces regression of the aberrant blood supply and normalizes blood vessels. An anti-Vegf therapy is also effective to restrain tumor growth and improves vascular remodeling. Importantly, only the combination treatment suppresses intratumoral hemorrhage and restores blood vessel perfusion, suggesting that it might represent an attractive therapy targeting tumor vasculature PMID: 28152577
  76. Selected Nanobodies specifically reacted to mVEGF, but cross-reactivity with other antigens was not observed. Evaluated affinity for the Nanobodies was in nanomolar range. PMID: 27167350
  77. VEGF-A mRNA and protein expression were upregulated in choroidal neovascularization; these changes were ameliorated by restoration of miR-93 by means of transfection. PMID: 27349759
  78. These results indicate that tensile force induces in vivo gene expression associated with vascularization early in tensile-force-induced sutural bone formation. Moreover, the early induction of Vegf gene expression is regulated by CTGF and ROCK2. PMID: 26825658
  79. Combining VEGF165 obviously enhanced the inducing effects of BMP2 on osteogenic differentiation capacity of C3H10T1/2 cells. PMID: 26757978
  80. our results clearly indicate that the two major VEGF isoforms are not redundant in inducing and regulating tumor angiogenesis, as they can exert even opposite effects on the growth and invasion of cancer cells in vivo. PMID: 27033458
  81. Endothelial cell-specific endoglin expression in islets of Langerhans is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size. PMID: 27456002
  82. PLD2 functions as a key mediator in the VEGF-mediated angiogenic functions of endothelial cells. PMID: 26818087
  83. These experiments reveal that transient, high-fat diet -elicited reduction of brain glucose uptake initiates a compensatory increase of VEGF production and assign obesity-associated macrophage activation a homeostatic role to restore cerebral glucose metabolism, preserve cognitive function, and limit neurodegeneration in obesity. PMID: 27133169
  84. IL-35 treatment reduced collagen-induced arthritis via inhibiting vascular endothelial growth factor and its receptors PMID: 26922678
  85. VEGF enhances glycolysis in pancreatic cancer via HIF1alpha up-regulation. PMID: 26980697
  86. Repeat administration of shRNA-Vegf-A moderately improves vascularremodeling associated with hemodial- ysis AVFs. PMID: 26948326
  87. The IMQ-induced mouse psoriatic model showed an upregulation of VEGF in the skin lesion. PMID: 26733387
  88. In lymphatic endothelial cells, ectodomain shedding of LYVE-1 was induced by vascular endothelial growth factor (VEGF)-A, an important factor for angiogenesis and lymphangiogenesis under pathological conditions. VEGF-A-induced LYVE-1 ectodomain shedding was mediated via the extracellular signal-regulated kinase (ERK) and a disintegrin and metalloproteinase (ADAM) 17. PMID: 26966180
  89. VEGF inhibitors bevacizumab improves blood flow recovery through the induction of PDGF-BB in a diabetic mouse hindlimb ischemia model. PMID: 26808210
  90. These findings suggest a shared pathogenic role of VEGF-A-induced and NLRP3 inflammasome-mediated IL-1beta activation for multiple distinct ocular aging diseases. PMID: 26912740
  91. miR-320a play important roles in doxorubicin induced cardiotoxicity by targeting VEGF signaling pathway. PMID: 26837315
  92. data demonstrate that the PI3K p110alpha-Akt/Rac1 and NOX1 signalling pathways play a pivotal role in VEGF-induced vascular differentiation and cell migration. PMID: 26553657
  93. Our findings show that PGC-1alpha control of ROS homeostasis plays an important role in the control of endothelial response to VEGF-A. PMID: 26828021
  94. Metformin inhibits breast tumor angiogenesis and suggest role of HIF-1alpha-VEGF signaling axis in mediating HER2-induced tumor angiogenesis. PMID: 26625311
  95. results suggest that sustained intraocular VEGF neutralization induces retinal neurodegeneration and vascular damage in the diabetic eye. PMID: 26671074
  96. VEGF-dependent capillary maintenance supports muscle growth. PMID: 26542520
  97. Data suggest that expression of Notch-1 receptor in endothelial progenitor cells (EPC) in bone marrow is up-regulated in diabetic angiopathy (DA); signaling in EPC via Notch-1 receptor and VEGFA/VEGFR2 appears to be altered in DA. PMID: 26598222
  98. Both placental NRP1 and VEGF were expressed at lower levels in women with pre-eclampsia and homocysteine-treated mice, which may contribute to endothelial damage. PMID: 26708340
  99. Collectively, these data suggest that VEGF/VEGFR2 signaling regulates germ cell proliferation and promotes testicular regeneration via direct action on germ cells and the enhancement of vascularization. PMID: 26727223
  100. RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix. PMID: 27126736

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Subcellular Location Isoform VEGF-1: Secreted, SUBCELLULAR LOCATION: Isoform VEGF-2: Secreted, SUBCELLULAR LOCATION: Isoform VEGF-3: Cell membrane, Peripheral membrane protein
Protein Families PDGF/VEGF growth factor family
Tissue Specificity In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in ki
Database Links

KEGG: mmu:22339

STRING: 10090.ENSMUSP00000115883

UniGene: Mm.282184

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