Mouse adiponectin,ADP ELISA Kit

Instructions
Code CSB-E07272m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name adiponectin, C1Q and collagen domain containing
Alternative Names Adipoq ELISA Kit; Acdc ELISA Kit; Acrp30 ELISA Kit; Apm1 ELISA Kit; Adiponectin ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; ACRP30 ELISA Kit; Adipocyte ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; Adipocyte-specific protein AdipoQ ELISA Kit
Abbreviation ADP
Uniprot No. Q60994
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Gene References into Functions
  1. These data suggest that APN has a moderate regulatory role in oxidative stress-induced mitophagy and suppresses apoptosis. These findings demonstrate the antioxidant potential of APN in oxidative stress-associated skeletal muscle diseases. PMID: 28600493
  2. Downregulation of adiponectin in inflamed adipocyte by fetuin-A through the mediation of Wnt3a and PPARgamma is a new report. PMID: 27720679
  3. Study results indicate for the first time that adiponectin is able to influence the mechanical responses in strips from the mouse gastric fundus. PMID: 30254407
  4. miR-711, which is upregulated by Adipoq, represses TLR4 signaling, acting therefore as a major mediator of the anti-inflammatory action of Adipoq. PMID: 28240307
  5. both paracrine and endocrine effects of adiponectin may contribute to reduced reactive oxygen species generation and apoptosis after MI/R, in a CD36-dependent manner PMID: 29018142
  6. These data may indicate that insulin resistance in Adp(-/-) mice is likely caused by an increase in concentrations of TNFalpha and FFA via downregulation of PPARalpha. PMID: 29445073
  7. Adiponectin improves metabolic health but has only minor effects on reproductive functions in the polycystic ovary syndromemouse model. PMID: 28790184
  8. APN attenuates adverse cardiac remodeling following cardiac injury by up-regulating MMP-9 expression. APN up-regulates MMP-9 expression via activation of AMPK and ERK1/2. PMID: 29263115
  9. the effects of APN on the promotion of preadipocyte differentiation under inflammatory conditions may involve the PPARgamma signaling pathway, and at least partly depends on upregulation of PPARgamma expression. PMID: 29115433
  10. Results demonstrate that adiponectin enhances inhibitory postsynaptic current onto neuropeptide Y (NPY) neurons to attenuate action potential firing in NPY neurons in a glucose-independent manner, being contrasted to its glucose-dependent effect on proopiomelanocortin neurons. PMID: 28606559
  11. Results show a reciprocal regulation of adiponectin and FGF19 gene expression in mice. PMID: 27666676
  12. Acrp30 (a globular form of adiponectin) reduces the expression of proinflammatory cytokines and the expression of RAGE as beta amyloid transporters into brain. Moreover, Acrp30 attenuated the apoptosis and the tight junction disruption through AdipoR1-mediated NF-kappaB pathway in beta amyloid-exposed bEnd.3 cells. PMID: 29022894
  13. Findings demonstrate that adiponectin is an essential regulator of thermogenesis, and adiponectin is required for maintaining body temperature under cold exposure. PMID: 29058611
  14. Chronic stress accelerates DPP4-mediated GLP-1 degradation and alters plasma adiponectin, accelerating vascular senescence and impairing ischemia-induced neovascularization. PMID: 28963101
  15. AdipoQ antisense (AS) Long noncoding RNA (lncRNA) transfer from nucleus to cytoplasm inhibits adipogenesis through formation of an AdipoQ AS lncRNA/AdipoQ mRNA duplex to suppress the translation of AdipoQ mRNA.[ PMID: 29414510
  16. Adiponectin enhances quiescence exit of murine hematopoietic stem cells and hematopoietic recovery through mTORC1 potentiation. PMID: 28480607
  17. plasma adiponectin and leptin were also decreased in IL 10tm.These findings suggest that frailty observed in this mouse model of chronic inflammation may in part be driven by alterations in fat mass, hormone secretion and energy metabolism PMID: 29267271
  18. CpG ODNs decreased placental adiponectin expression in NOD mice and impaired human trophoblast function and was associated with increased embryo loss. Adiponectin may therefore play an important protective role in the prevention of bacteria-induced pregnancy failure. PMID: 27094728
  19. Caloric restriction (CR) impacted adiposity but only levels of the high molecular weight isoform of adiponectin responded to CR. PMID: 28156058
  20. T-cadherin was essential for accumulation of adiponectin in the neointima and atherosclerotic plaque lesions, and the adiponectin-T-cadherin association protected against vascular injury. PMID: 28062540
  21. mTORC1 mediated many of the beneficial actions of FGF21 in vitro, including UCP1 and FGF21 induction, increased adiponectin secretion, and enhanced glucose uptake without any adverse effects on insulin action. PMID: 27681418
  22. AnxA6 protein in adipocytes was upregulated by oxidative stress which might trigger AnxA6 induction in adipose tissues and contribute to impaired fat storage and adiponectin release. PMID: 27702590
  23. Adiponectin alters calcium and phosphate balance and renal mineral excretion, in part, through klotho. PMID: 27914707
  24. Adiponectin (ApN) proves to be a powerful protector of the skeletal muscle capable of reversing the disease progression, thus making it a potential therapeutic agent for Duchenne muscular dystrophy (DMD). PMID: 28463682
  25. These results demonstrated that LC along with insulin increases GSH levels thereby improving adiponectin secretion and glucose utilization in adipocytes. PMID: 28755973
  26. Adiponectin, TNF-alpha, and LOX-1 exert complex regulatory effects on the coronary microvascular endothelial function in atherosclerotic ApoE knockout mice. PMID: 27050429
  27. adiponectin inhibited endoplasmic reticulum stress and apoptosis of adipocyte in vivo and in vitro by activating the AMPK/PPARalpha/ATF2 pathway. PMID: 27882945
  28. Irisin improved endothelial function by modulating HO-1/ adiponectin axis in perivascular adipose tissue (PVAT) in HFD-induced obese mice. These findings suggest that regulating PVAT function may be a potential mechanism by which irisin improves endothelial function in obesity. PMID: 28595178
  29. a unique key feature of the T-cad prodomain is its involvement in binding of the T-cad repeats 1 and 2 to adiponectin; adiponectin positively regulates T-cad abundance PMID: 28325833
  30. adiponectin maintains intestinal homeostasis and protects against murine colitis through interactions with its receptor AdipoR1 and by modulating adaptive immunity and STAT3 signaling PMID: 28258220
  31. The KIF5B level was up-regulated during 3T3-L1 adipogenesis. This increase in cytosolic KIF5B was synchronized with the induction of adiponectin. Endogenous KIF5B and adiponectin were partially colocalized at the peri-nuclear and cytosolic regions. PMID: 27264953
  32. The elevation in circulating levels of adiponectin and Fgf15 led to normalized hepatic and serum levels of bile acids, limited hepatic accumulation of toxic bile, attenuated inflammation, and amelioration of liver injury in the ethanol-fed mNT knockout mice. PMID: 27573244
  33. Female adiponectin null mice displayed impaired fertility, reduced oocytes, disrupted estrous cycle, increased atretic follicles, and impaired late folliculogenesis. There was decrease in serum estradiol and FSH but an increase in LH and testosterone at proestrus. There was reduction of progesterone levels at diestrus, a significant decrease in LH receptor expression as well as in the number of GnRH immunoreactive neurons PMID: 27700136
  34. Tongqiaohuoxue decoction improved obesity-induced inflammation and insulin resistance by increasing adiponectin production. PMID: 27404230
  35. High salt is an important suppressor of cardioprotective APN and AdipoR1 in cardiac myocytes. PMID: 28051329
  36. PPARdelta activation in perirenal fat by agonist or high sodium intake inhibited renal sodium-glucose cotransporter 2 (SGLT2) function, which is mediated by increased production of adipose adiponectin. PMID: 27053360
  37. Acute knockdown of Insr or both Irs1 and Irs2 in adipocytes increased Adipoq mRNA expression but reduced adiponectin secretion. PMID: 26888756
  38. Adiponectin may protect the aorta from atherosclerotic injury by reducing inflammation. Adiponectin effectively inhibits the activation of NF-kappa B pathway by inhibiting the expression of NF-kappa B nuclear protein p65. PMID: 26965176
  39. Altered adiponectin multimerization could explain declined adiponectin levels and altered distribution of adiponectin complexes in the plasma of obese insulin-resistant individuals PMID: 26407855
  40. The expression of PI3K-insensitive GSK3 stimulates the production of adiponectin and protects from diet-induced metabolic syndrome. PMID: 27140617
  41. These data suggest that adiponectin could represent a possible biomarker in obesity-associated asthma PMID: 26462929
  42. ADPN might act as a biomarker of inflammation and have potential for the treatment of hemorrhagic shock. PMID: 26909947
  43. Adiponectin exerts novel effects to limit the production and action of mono-MPs, underscoring yet another pleiotropic effect of this adipokine. PMID: 26687997
  44. The results demonstrated a dynamic dysfunction of APN/AdipoR1 axis accompanying progression of diabetes mellitus in mice with cerebral ischemia. PMID: 26611106
  45. reduced plasma levels in obese mice treated with house dust mite allergens as compared to similarly treated lean mice PMID: 26476732
  46. CRP decreased adiponectin expression and multimerization, while CRP-induced decline in adiponectin might be mediated through the PI3K/Akt pathway. PMID: 26812237
  47. Globular Adiponectin (gAd) activates autophagy in myoblasts and gAd-activated autophagy drives the myogenic properties of this hormone. PMID: 26826036
  48. APN can help to reduce periodontitis-related bone loss, modulate JMJD3 and IRF4 expression, and macrophage infiltration. PMID: 26399931
  49. our data indicate that robust hypertrophic MEF2 activation in the heart in vivo requires a background of adiponectin signaling and that adiponectin signaling in primary isolated CM directly enhances MEF2 activity through activation of p38 MAPK PMID: 26196305
  50. In 3T3-L1 adipocytes, catechin and quercetin attenuated TNF-alpha-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. PMID: 25620282
  51. These findings uncover adiponectin as a key efferent signal for cold-induced adaptive thermogenesis by mediating the crosstalk between adipocytes and M2 macrophages in scWAT. PMID: 26166748
  52. Results show that perivascular adipose tissue-secreted APN suppresses plaque formation by inducing macrophage autophagy via suppressing the Akt/FOXO3a signaling pathway. PMID: 26020520
  53. Adiponectin directly acts on murine dermal gammadelta-T cells to suppress IL-17 synthesis via AdipoR1. Mice with adiponectin deficiency show severe psoriasiform skin inflammation with enhanced infiltration of IL-17-producing dermal Vgamma4+gammadelta-T cells. PMID: 26173479
  54. DNA hypermethylation of a particular region of the adiponectin promoter suppresses adiponectin expression through epigenetic control and, in turn, exacerbates metabolic diseases in obesity. PMID: 26139044
  55. The chemical synthesis is described of a polypeptide construct possessing both the variable and the collagen-like domain of adiponectin. PMID: 24752567
  56. The aim of this study was to investigate the impact of the substitution at position 70 in hepatitis c virus core protein on adipokine production by murine and human adipocytes. PMID: 26121241
  57. ADIPOQ stimulates autophagic flux in skeletal muscle, which likely represents one important mechanism mediating multiple favorable metabolic effects PMID: 25905437
  58. adiponectin is an important determinant of the kidney response to high glucose in vivo and in vitro PMID: 25957229
  59. Mitochondrial and contractile function are preserved in hearts of mice lacking adiponectin, suggesting that adiponectin may be expendable in the regulation of mitochondrial energetics and contractile function in the heart under non-pathological conditions PMID: 25785965
  60. adiponectin. Taken together, our results show that adiponectin is stored in a unique vesicular compartment, and released through a regulated exocytosis pathway that is dependent on insulin signalling. PMID: 26330614
  61. maternal ADN supplementation reversed the adverse effects of maternal obesity on placental function and fetal growth. Improving maternal ADN levels PMID: 26417088
  62. these data indicate that increased myocardial bioavailability of adiponectin mediates ECM remodeling following PO and that adiponectin deficiency delays these effects. PMID: 25910275
  63. Elevated gAd at differentiation of 3T3-L1 cells leads to reduced lipid content, reduced lipid metabolism and high resistance to inflammation. PMID: 25491932
  64. Adipoq plays a part in the anti-aging, but not in the anti-tumor, effects of calorie restriction. PMID: 25698374
  65. ERp44 exclusively recognizes and converts assembly-trapped adiponectin intermediates back to precursors of the biologically potent high molecular weight form. PMID: 26060250
  66. Endoplasmic reticulum is an important site of adiponectin accumulation whose secretion is increased through the action of long chain omega-3 fatty acids. PMID: 25900100
  67. data also indicate that stimulation of omega-oxidation of fatty acids by the HFD is enhanced by adiponectin PMID: 25915851
  68. The cardioprotective effects of TNF-alpha neutralization are partially due to the upregulation of adiponectin. PMID: 25888509
  69. our findings suggest that APN may have a potential role in regulating soft tissue sarcoma growth. PMID: 25694398
  70. Adiponectin deletion impairs insulin signaling in insulin-sensitive but not insulin-resistant 3T3-L1 adipocytes. PMID: 25956568
  71. FGF21 induces adipocyte production of adiponectin which acts on the blood vessels to inhibit neointima formation and macrophage inflammation. Adiponectin treatment partly reverses atherosclerosis but not hypercholesterolemia in apo-E(-/-) mice. PMID: 25794851
  72. These data indicate that PHs and LHs are both required for physiological adiponectin production and in particular are essential for the formation/secretion of the HMW isoforms. PMID: 25240468
  73. adiponectin expression is regulated by the circadian clock and through the circadian expression of its transcription factor PPARgamma and its co-activator PGC1alpha. PMID: 25448847
  74. our data presented a novel role for VDRA and ACEI in reducing factors associated with CHD that may lead to the discovery of new therapeutic venues. PMID: 25037058
  75. elevated adiponectin levels improve systemic lipid metabolism in the near absence of insulin. PMID: 25339419
  76. adiponectin elicited Mer expression and Mer-dependent efferocytosis in macrophages similar to cells stimulated with C1q. PMID: 24942043
  77. our findings reveal adiponectin as a dosage-dependent regulator of lactation homeostasis and milk quality that critically controls inflammation in the nursing neonates. PMID: 25590242
  78. Adiponectin suppressed mesangial cell proliferation and extracellular matrix production,thus suppressing the development and progression of diabetic nephropathy. PMID: 24816832
  79. DsbA-L is localized in both the mitochondria and the endoplasmic reticulum (ER) in adipocytes; its ER localization plays a critical role in suppressing ER stress and promoting adiponectin biosynthesis and secretion. PMID: 25739441
  80. Adiponectin prevents pancreatic beta-cells from apoptosis by inhibition of intrinsic apoptosis pathway via regulation of the BCL2 family. PMID: 25028793
  81. APN has an important role in preventing age-related hearing impairment. PMID: 24763046
  82. These results suggest that adiponectin protects against LPS-induced acute cardiac injury by suppressing cardiac inflammatory responses, and could represent a potential therapeutic target in sepsis-associated myocardial dysfunction. PMID: 25180179
  83. Data indicate that adiponectin increase mitochondrial biogenesis and inhibit apoptosis of pancreatic cancer cells via AMP-activated protein kinase (AMPK)/sirtuin-1 (Sirt1)/peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1alpha). PMID: 25051362
  84. These data show that both circulating and tissue-bound Adipo levels are dependent on Tcad and, in reverse, regulate tissue Tcad levels through a positive feedback loop. PMID: 25514086
  85. These data suggest that adiponectin may play a significant role in mediating the effects of exercise on hippocampal neurogenesis and depression, possibly by activation of the ADNR1/AMPK signaling pathways PMID: 25331877
  86. study found adiponectin attenuates Ang II-induced vascular inflammation and abdominal aortic aneurysms formation in mice PMID: 24911638
  87. This study demonstrates that blocking KHK and redirecting fructose metabolism to alternative pathways is an effective way to prevent visceral obesity and insulin resistance induced by high fructose, a widespread component of Western diets. PMID: 25187370
  88. Sphingosine 1-phosphate is a novel regulator of SERCA2 that activates CaMKII signaling and mediates adiponectin-induced cardioprotection. PMID: 24852843
  89. We have provided the first direct evidence that APN is a novel regulator of PP2Cm and systematic branched chain amino acid levels. PMID: 25071024
  90. Adiponectin stimulated skeletal muscle autophagy and antioxidant potential to reduce insulin resistance caused by high fat diet. PMID: 25071026
  91. The findings identify A20 as a mediator of adiponectin anti-inflammatory action in white adipose tissue and a potential target for mitigating obesity-related pathology. PMID: 25190567
  92. documented that either adiponectin or the synthetic peptide with adiponectin properties, ADP355, suppressed p-FAK in synthetic matrices with stiffness measurements of 9 and 15 kPa PMID: 25154876
  93. Adiponectin is not required for the maintenance of mitochondrial content, or for exercise-induced increases in skeletal muscle mitochondrial proteins. PMID: 24687585
  94. suppressed adiponectin expression/production by MI or TNFalpha administration was markedly decreased by TNFR1 deletion (P<0.01 vs. WT), but exacerbated by TNFR2 deletion (P<0.05 vs. WT). PMID: 23465561
  95. adiponectin suppresses thermogenesis, which is likely to be a mechanism whereby adiponectin reduces energy expenditure PMID: 24531262
  96. A link between mitochondrial biogenesis and dynamics and adiponectin synthesis in white adipocytes, is reported. PMID: 24604890
  97. Adiponectin promotes CVB3 myocarditis by suppressing TLR-dependent innate immunity, polarization of anti-inflammatory M2 macrophages and reduction of number and activation of NK cells resulting in attenuated acute anti-viral immune responses. PMID: 24691762
  98. Adiponectin expression protects against angiotensin II-mediated inflammation and accelerated atherosclerosis. PMID: 24466075
  99. Data suggest adiponectin-AMPK (AMP-activated protein kinase) signaling can be modulated by dietary factors; here, dietary supplement camphene protects against hepatic steatosis/insulin resistance via activation of adiponectin-AMPK signaling in liver. PMID: 23818423
  100. Adiponectin regulates ACTH secretion and the hypothalamic-pituitary-adrenal axis in an AMPK-dependent manner in pituitary corticotroph cells. PMID: 24361598

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Subcellular Location Secreted
Tissue Specificity Synthesized exclusively by adipocytes and secreted into plasma.
Database Links

KEGG: mmu:11450

STRING: 10090.ENSMUSP00000023593

UniGene: Mm.3969

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