Rat Interleukin 4,IL-4 ELISA KIT

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Citations

Customer Reviews and Q&A

Target Background

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Rat Interleukin 4,IL-4 ELISA KIT

Name: Rat Interleukin 4,IL-4 ELISA KIT
Brand: CUSABIO
SKU: CSB-E04635r

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Citations (39) Protocol MSDS

Code	CSB-E04635r
Size	$395
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Product Details
Citations (39)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

interleukin 4

Alternative Names

Il4 ELISA Kit; Il-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell IGG differentiation factor ELISA Kit; B-cell growth factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit

Abbreviation

IL4

Uniprot No.

P20096

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-110
1:2	Average %	97
	Range %	91-105
1:4	Average %	99
	Range %	94-104
1:8	Average %	93
	Range %	89-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

3.065

2.834

2.949

2.794

500

2.708

2.567

2.637

2.482

250

2.135

2.028

2.082

1.926

125

1.434

1.429

1.431

1.276

62.5

0.774

0.721

0.748

0.592

31.2

0.468

0.453

0.461

0.305

15.6

0.307

0.294

0.300

0.145

0.156

0.155

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

CUSABIO's rat IL-4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of IL-4 concentrations in serum, plasma, and tissue homogenates. This assay exclusively recognizes rat IL-4. The quantitative sandwich ELISA technique of this kit is based on IL-4 antibody-IL-4 antigen interactions and an HRP colorimetric detection system to detect the levels of IL-4 in samples. The intensity of the color is positively proportional to the amount of bound IL-4 in the initial step.

IL-4 is a pleiotropic Th2 cytokine involved in IgE production, mast cell development, and eosinophil development. IL-4 binds to IL-4Rα-γc or IL-4Rα-IL-13Rα1, activating JAKs, leading to the recruitment of mediators of cell growth, resistance to apoptosis, gene activation, and differentiation. Dysregulated IL-4 signaling is associated with the pathophysiology of inflammatory and allergic disorders, such as asthma and atopic dermatitis (AD). IL-4 is implicated in asthma through its involvement in IgE production, eosinophil chemotaxis, and the differentiation of THO cells to TH2 cells. It also plays an essential role in the development of allergic diseases. IL4/IL4R signaling also exerts immune and pro-tumor functions.

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Dietary Methionine Restriction Improves Gut Health and Alters the Plasma Metabolomic Profile in Rats by Modulating the Composition of the Gut Microbiota M Yang,/,2024
Three Birds, One Stone: An Osteo-Microenvironment Stage-Regulative Scaffold for Bone Defect Repair through Modulating Early Osteo-Immunomodulation, Middle Neovascularization, and Later Osteogenesis Y Yuan,Advanced science,2023
Stem cell biotherapy: A new remedy for Trichinella spiralis-induced inflammatory myopathy DM Abou Rayia,Parasitology international,2023
Mesenchymal Stem Cells and Myeloid-Derived Suppressor Cells Interplay in Adjuvant-Induced Arthritis Rat Model FAM Abo-Aziza,International immunopharmacology,2023
Taurine alleviated hepatic steatosis in oleic acid-treated-HepG2 cells and rats fed a high-fat diet Q Song,Heliyon,2023
Tongue‐brain‐transported ZnO NPs induced abnormal taste perception A Chen,Advanced healthcare materials,2023
Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent Preeclampsia H Liu,Cell transplantation,2023
Xiaoqinglong Decoction Enhances Autophagy to Antagonist Airway Inflammation Induced by Cold in Asthmatic Rats B Wang,Evidence-based complementary and alternative medicine,2022
In Vivo Immune Study of Achillea fragrantissima Extract Versus Echinaid and Endoxan in Wistar Rats RM Alhomaid,Current Research in Nutrition and Food Science Journal,2022
Influenza a virus triggers acute exacerbation of chronic obstructive pulmonary disease by increasing proinflammatory cytokines secretion via NLRP3 inflammasome activation S Ji,Journal of inflammation,2022
IL-10/STAT3 signalling pathway-mediated T-cell suppressors upregulation in severe HFMD with EV71 infection Yaozhong Zhang,Research Square,2022
Improved cognition impairment by activating cannabinoid receptor type 2: Modulating CREB/BDNF expression and impeding TLR-4/NFκBp65/M1 microglia signaling pathway in D-galactose-injected ovariectomized rats SS Abd El-Rahman,PloS one,2022
Differential Immunomodulatory Potential of Silver Nanoparticles and Effect on the Kynurenine Pathway in Male Wistar Rats Lawrence Boluwatife Afolabi,Journal of Nanomaterial,2022
Lycopene Modulates Placental Health and Fetal Development Under High‐Fat Diet During Pregnancy of Rats S Sun,Molecular Nutrition & Food Research,2021
The effects of ursodeoxycholic acid on sepsis-induced cholestasis management in an animal model RH Ainosah,Journal of Taibah University Medical Sciences,2020
The prenatal challenge with lipopolysaccharide and polyinosinic:polycytidylic acid disrupts CX3CL1-CX3CR1 and CD200-CD200R signalling in the brains of male rat offspring: a link to schizophrenia-like behaviours K Chamera,Journal of Neuroinflammation,2020
Maternal Immune Activation Sensitizes Male Offspring Rats to Lipopolysaccharide-Induced Microglial Deficits Involving the Dysfunction of CD200–CD200R and CX3CL1–CX3CR1 Systems K Chamera,Cells,2020
Metoclopramide use to induce lactation can alter DRD2 and BDNF in the prefrontal cortex of offspring SG Gürgen,Journal of Chemical Neuroanatomy,2020
SiRNA in MSCs-Derived Exosomes Silences Ctgf Gene for Corticospinal Axon Regeneration and Locomotor Recovery in Spinal Cord Injury Rats W Huang,Research Square Preprint,2020
Potential anti-inflammatory and immunomodulatory effects of carvacrol against ovalbumin-induced asthma in rats El-Din Y M E,Life Sciences,2019
Anti-obesity effect of cocoa proteins (Theobroma cacao L.) variety “Criollo” and the expression of genes related to the dysfunction of white adipose tissue in high-fat diet-induced obese rats Luis JorgeCoronado-Caceres, et al,Journal of Functional Foods,2019
BTLA suppress acute rejection via regulating TCR downstream signals and cytokines production in kidney transplantation and prolonged allografts survival Jiayi Zhang,, et al,Scientific Reports,2019
In vitro-in vivo correlation of inhalable budesonide-loaded large porous particles for sustained treatment regimen of asthma Li J, et al,Acta Biomaterialia,2019
Immunosuppressive effect of artemisinin and hydroxychloroquine combination therapy on IgA nephropathy via regulating the differentiation of CD4+ T cell subsets in rats Bai L, et al,International immunopharmacology,2019
Brusatol ameliorates 2, 4, 6-trinitrobenzenesulfonic acid-induced experimental colitis in rats: Involvement of NF-κB pathway and NLRP3 inflammasome JiangtaoZhou.et al,International Immunopharmacology,2018
The Cu-containing TiO2 coatings with modulatory effects on macrophage polarization and bactericidal capacity prepared by micro-arc oxidation on titanium substrates QianliHuang.et al,Colloids and Surfaces B: Biointerfaces,2018
Sustained therapeutic efficacy of budesonide-loaded chitosan swellable microparticles after lung delivery: Influence of in vitro release, treatment interval and dose Lan Zhang.et al,Journal of Controlled Release,2018
IL-4 mediates the delayed neurobehavioral impairments induced by neonatal hepatitis B vaccination that involves the down-regulation of the IL-4 receptor in the hippocampus XiaoWang.et al,Cytokine,2018
Mahuang Fuzi XixinDecoction Attenuates Th1 and Th2 Responses in Treatment of Ovalbumin-induced Allergic Inflammation in Rat Model of Allergic Rhinitis Mengyue Ren.et al,/,/
Effects of the combined extracts of Herba Epimedii and Fructus Ligustrilucidi on airway remodeling in the asthmatic rats with the treatment of budesonide Tang X.et al,BMC Complement Altern Med.,2017
Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity L.V. Fedulova.et al,Journal of Pharmacy and Nutrition Sciences,2017
Liuweibuqi capsules suppress inflammation by affecting T cell polarization and survival in chronic obstructive pulmonary disease Chengyang Wang.et al,Medicinal Chemistry Research,2017
Impaired function of the intestinal barrier in a novel sub-health rat model. Sisi Feng.et al,Molecular Medicine Reports,2016
Anti-diabetic effects of soluble and insoluble dietary fibre from deoiled cumin in low-dose streptozotocin and high glucose-fat diet-induced type 2 diabetic rats Ma M.et al,Journal of Functional Foods,2016
Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma. Johnathan M.et al,BMC Complement Altern Med.,2016
Synthetic p55 tandem DNA vaccine against Pneumocystis carinii in rats. Fan H.et al,Microbiol Immunol.,2016
Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti Hoan TD. et al,Exp Parasitol,2015
Immunostimulative effects of Cyperus rotundus, Alpinia calcarata, Solanum surattense, Clerodendrum infortunatum and Croton laccifer extracts combination on cyclophosphamide-induced immunosupression in rats Ediriweera P. S. et al,Journal of Experimental and Integrative Medicine,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

Gene References into Functions

age-related decrease in IL-4 expression exacerbated surgery-induced cognitive deficits and exaggerated the neuroinflammatory responses PMID: 28414034
IL-4 Release from a Biomimetic Scaffold for the Temporally Controlled Modulation of Macrophage Response PMID: 26951461
Formoterol synergizes with des-ciclesonide to inhibit IL-4 expression in IgE/antigen-induced mast cells by inhibiting JNK activation. PMID: 26003274
CaSR activation promoted T-cell apoptosis and the secretion of pro-inflammatory cytokine TNF-alpha and anti-inflammatory cytokines IL-4. PMID: 25256599
IL-4 was found to augment the mRNA expression of ICAM-1. PMID: 23904363
IL-4 has a modulatory effect on the enzymatic compartment of the antioxidant defense system in the brain of rats. PMID: 24319732
IL-4 promoted the activation of effector cells to mediate rejection and did not support alloantigen-specific Treg that could transfer specific tolerance. PMID: 24139939
The level of IL-4 is elevated in masseter muscle of both sexes following chemically induced inflammation. PMID: 20969918
Treatment with intranasal interferon gamma can inhibit production of IL4 and IL5 in allergic rhinitis. PMID: 18510219
IL4 in bronchoalveolar lavage fluid of allergic rhinitis rats was elevated. PMID: 16229173
endogenous expression of antiinflammatory cytokine IL-4 regulates the extent of acute macrophage activation and confines the ensuing secondary cavity formation after spinal cord trauma PMID: 20623539
passive rats exposed to emotional stress were characterized by a decreased blood levels of TNF alpha and IL-4 as compared to behaviorally passive controls and behaviorally active animals exposed to stress. PMID: 20120734
A single treatment with IL-4 via retrovirally transduced lymphocytes partially protects against type 1 diabetes in BioBreeding rats. PMID: 12004164
Gene transfer of IL-4 may represent a potential therapeutic approach to induce tolerance to splenic allografts. PMID: 15251393
IL-4 appears to be an important Th2 cytokine involved in the induction of the L-arginine/iNOS pathway in eosinophils PMID: 15826818
synthesis and release from mast cells mediated by Mycoplasma pneumoniae P1 adhesin PMID: 16169702
HSV-mediated expression of IL-4 effectively reduces the behavioral manifestations of neuropathic pain, and reverses some of the biochemical and histologic correlates of neuropathic pain at the spinal level. PMID: 16503976
Data show that islets of non-diabetic BB rats become positive to both IL-1beta and IL-4 very early on, and that production of these interleukins together with the expression of CD4 and CD25 are predictive for type 1 diabetes onset in non-diabetic BB rats. PMID: 16972262
These data suggest that IL-4 may provide a significant immunomodulatory signal which can protect against microglia-mediated neurotoxicity by suppressing the production and release of free radicals. PMID: 17018025
We conclude that IL-4 exerts its anti-inflammatory effects in a limited and target-specific manner, which is delicately regulated by the cellular microenvironment. T PMID: 17442043
Our data demonstrate, for the first time, the neuroprotective effect of IL-4 through cAMP/PKA pathway in thapsigargin-induced photoreceptor cell death. PMID: 19235892

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Subcellular Location

Secreted.

Protein Families

IL-4/IL-13 family

Database Links

KEGG: rno:287287

STRING: 10116.ENSRNOP00000010029

UniGene: Rn.108255

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Related pathways

Jak-STAT signaling pathway PI3K-Akt signaling pathway Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation

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