Rat Interleukin 4,IL-4 ELISA KIT

Code CSB-E04635r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


CUSABIO's rat IL-4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of IL-4 concentrations in serum, plasma, and tissue homogenates. This assay exclusively recognizes rat IL-4. The quantitative sandwich ELISA technique of this kit is based on IL-4 antibody-IL-4 antigen interactions and an HRP colorimetric detection system to detect the levels of IL-4 in samples. The intensity of the color is positively proportional to the amount of bound IL-4 in the initial step.

IL-4 is a pleiotropic Th2 cytokine involved in IgE production, mast cell development, and eosinophil development. IL-4 binds to IL-4Rα-γc or IL-4Rα-IL-13Rα1, activating JAKs, leading to the recruitment of mediators of cell growth, resistance to apoptosis, gene activation, and differentiation. Dysregulated IL-4 signaling is associated with the pathophysiology of inflammatory and allergic disorders, such as asthma and atopic dermatitis (AD). IL-4 is implicated in asthma through its involvement in IgE production, eosinophil chemotaxis, and the differentiation of THO cells to TH2 cells. It also plays an essential role in the development of allergic diseases. IL4/IL4R signaling also exerts immune and pro-tumor functions.

Target Name interleukin 4
Alternative Names Il4 ELISA Kit; Il-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell IGG differentiation factor ELISA Kit; B-cell growth factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
Abbreviation IL4
Uniprot No. P20096
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Target Background

(From Uniprot)
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Gene References into Functions
  1. age-related decrease in IL-4 expression exacerbated surgery-induced cognitive deficits and exaggerated the neuroinflammatory responses PMID: 28414034
  2. IL-4 Release from a Biomimetic Scaffold for the Temporally Controlled Modulation of Macrophage Response PMID: 26951461
  3. Formoterol synergizes with des-ciclesonide to inhibit IL-4 expression in IgE/antigen-induced mast cells by inhibiting JNK activation. PMID: 26003274
  4. CaSR activation promoted T-cell apoptosis and the secretion of pro-inflammatory cytokine TNF-alpha and anti-inflammatory cytokines IL-4. PMID: 25256599
  5. IL-4 was found to augment the mRNA expression of ICAM-1. PMID: 23904363
  6. IL-4 has a modulatory effect on the enzymatic compartment of the antioxidant defense system in the brain of rats. PMID: 24319732
  7. IL-4 promoted the activation of effector cells to mediate rejection and did not support alloantigen-specific Treg that could transfer specific tolerance. PMID: 24139939
  8. The level of IL-4 is elevated in masseter muscle of both sexes following chemically induced inflammation. PMID: 20969918
  9. Treatment with intranasal interferon gamma can inhibit production of IL4 and IL5 in allergic rhinitis. PMID: 18510219
  10. IL4 in bronchoalveolar lavage fluid of allergic rhinitis rats was elevated. PMID: 16229173
  11. endogenous expression of antiinflammatory cytokine IL-4 regulates the extent of acute macrophage activation and confines the ensuing secondary cavity formation after spinal cord trauma PMID: 20623539
  12. passive rats exposed to emotional stress were characterized by a decreased blood levels of TNF alpha and IL-4 as compared to behaviorally passive controls and behaviorally active animals exposed to stress. PMID: 20120734
  13. A single treatment with IL-4 via retrovirally transduced lymphocytes partially protects against type 1 diabetes in BioBreeding rats. PMID: 12004164
  14. Gene transfer of IL-4 may represent a potential therapeutic approach to induce tolerance to splenic allografts. PMID: 15251393
  15. IL-4 appears to be an important Th2 cytokine involved in the induction of the L-arginine/iNOS pathway in eosinophils PMID: 15826818
  16. synthesis and release from mast cells mediated by Mycoplasma pneumoniae P1 adhesin PMID: 16169702
  17. HSV-mediated expression of IL-4 effectively reduces the behavioral manifestations of neuropathic pain, and reverses some of the biochemical and histologic correlates of neuropathic pain at the spinal level. PMID: 16503976
  18. Data show that islets of non-diabetic BB rats become positive to both IL-1beta and IL-4 very early on, and that production of these interleukins together with the expression of CD4 and CD25 are predictive for type 1 diabetes onset in non-diabetic BB rats. PMID: 16972262
  19. These data suggest that IL-4 may provide a significant immunomodulatory signal which can protect against microglia-mediated neurotoxicity by suppressing the production and release of free radicals. PMID: 17018025
  20. We conclude that IL-4 exerts its anti-inflammatory effects in a limited and target-specific manner, which is delicately regulated by the cellular microenvironment. T PMID: 17442043
  21. Our data demonstrate, for the first time, the neuroprotective effect of IL-4 through cAMP/PKA pathway in thapsigargin-induced photoreceptor cell death. PMID: 19235892

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Subcellular Location Secreted.
Protein Families IL-4/IL-13 family
Database Links

KEGG: rno:287287

STRING: 10116.ENSRNOP00000010029

UniGene: Rn.108255

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