Code | CSB-E07435r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 85 | ||||
Range % | 82-91 | |||||
1:2 | Average % | 92 | ||||
Range % | 83-99 | |||||
1:4 | Average % | 101 | ||||
Range % | 93-107 | |||||
1:8 | Average % | 96 | ||||
Range % | 90-104 |
The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 88 | 85-94 | ||||
EDTA plasma (n=4) | 94 | 90-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Interleukin 5 is a cytokine that regulates eosinophil development, activation, and survival. As a member of the type I cytokine family, IL-5 is primarily produced by T helper 2 cells, mast cells, and eosinophils themselves. This glycoprotein acts as a key mediator in allergic inflammatory responses and parasitic infections. It promotes eosinophil differentiation from bone marrow precursors and enhances their recruitment to sites of inflammation. IL-5 signaling is particularly important in asthma, allergic rhinitis, and other eosinophil-associated disorders, making it a significant target for therapeutic intervention and biomarker research.
The Rat Interleukin 5,IL-5 ELISA KIT (CSB-E07435r) is designed for quantitative measurement of IL-5 in rat samples using a sandwich assay principle. This kit works with various sample types including serum, plasma, cell culture supernates, and tissue homogenates with a sample volume requirement of 50-100ul. The assay provides a detection range of 15.6 pg/mL to 1000 pg/mL with a sensitivity of 3.9 pg/mL. Results are measured at 450 nm wavelength. The complete assay can be performed within 1-5 hours.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
Based on the provided information, no published research applications could be identified for this rat interleukin 5 ELISA kit from the available citation excerpts. Without specific research examples, a comprehensive applications summary cannot be generated at this time.
• Inflammatory research - Potential applications in studies examining eosinophil-mediated immune responses and allergic inflammation pathways
• Respiratory disease studies - Possible use in research examining asthma and other eosinophil-associated respiratory conditions
• Immunological profiling - Potential application in cytokine panel studies assessing immune system activation and regulation
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STRING: 10116.ENSRNOP00000010729
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