Rat Interleukin 5,IL-5 ELISA KIT

Code CSB-E07435r
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 5 (colony-stimulating factor, eosinophil)
Alternative Names
Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 82-91  
1:2 Average % 92  
Range % 83-99  
1:4 Average % 101  
Range % 93-107  
1:8 Average % 96  
Range % 90-104  
Recovery
The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 85-94  
EDTA plasma (n=4) 94 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.407 2.457 2.432 2.268  
500 1.768 1.736 1.752 1.588  
250 1.065 1.098 1.082 0.918  
125 0.704 0.735 0.720 0.556  
62.5 0.448 0.469 0.459 0.295  
31.2 0.323 0.300 0.312 0.148  
15.6 0.221 0.229 0.225 0.061  
0 0.161 0.167 0.164    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Interleukin 5 is a cytokine that regulates eosinophil development, activation, and survival. As a member of the type I cytokine family, IL-5 is primarily produced by T helper 2 cells, mast cells, and eosinophils themselves. This glycoprotein acts as a key mediator in allergic inflammatory responses and parasitic infections. It promotes eosinophil differentiation from bone marrow precursors and enhances their recruitment to sites of inflammation. IL-5 signaling is particularly important in asthma, allergic rhinitis, and other eosinophil-associated disorders, making it a significant target for therapeutic intervention and biomarker research.

The Rat Interleukin 5,IL-5 ELISA KIT (CSB-E07435r) is designed for quantitative measurement of IL-5 in rat samples using a sandwich assay principle. This kit works with various sample types including serum, plasma, cell culture supernates, and tissue homogenates with a sample volume requirement of 50-100ul. The assay provides a detection range of 15.6 pg/mL to 1000 pg/mL with a sensitivity of 3.9 pg/mL. Results are measured at 450 nm wavelength. The complete assay can be performed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

Based on the provided information, no published research applications could be identified for this rat interleukin 5 ELISA kit from the available citation excerpts. Without specific research examples, a comprehensive applications summary cannot be generated at this time.

Inflammatory research - Potential applications in studies examining eosinophil-mediated immune responses and allergic inflammation pathways
Respiratory disease studies - Possible use in research examining asthma and other eosinophil-associated respiratory conditions
Immunological profiling - Potential application in cytokine panel studies assessing immune system activation and regulation

Citations

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Target Background

Function
(From Uniprot)
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Subcellular Location
Secreted.
Protein Families
IL-5 family
Database Links
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