CD19 Monoclonal Antibody

Datasheet
Code CSB-MA004888A1m
Product Type Monoclonal Antibody
Size
Uniprot No. P15391
Image
  • Western Blot
    Positive WB detected in: Raji whole cell lysate
    All lanes: CD19 antibody at 1:2000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 61 kDa
    Observed band size: 95 kDa

  • IHC image of CSB-MA004888A1m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-MA004888A1m diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Overlay histogram showing Raji cells stained with CSB-MA004888A1m (red line) at 1:50. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunogen Recombinant Human CD19 protein (20-291AA)
Raised in Mouse
Species Reactivity Human
Tested Applications ELISA, WB, IHC, FC; Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200
Relevance Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein A purified
Isotype IgG2b
Clonality Monoclonal
Alias B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Gene Names CD19
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 4C6G1
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Function Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Involvement in disease Immunodeficiency, common variable, 3 (CVID3)
Subcellular Location Membrane, Single-pass type I membrane protein
Database Links

HGNC: 1633

OMIM: 107265

KEGG: hsa:930

STRING: 9606.ENSP00000437940

UniGene: Hs.652262

Pathway PI3K-Akt signaling pathway
B cell receptor signaling pathway
Hematopoietic cell lineage

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