IL1B Antibody

Code CSB-PA003023
Size US$167
  • Western Blot analysis of HeLa cells using IL-1β Polyclonal Antibody
  • Western Blot analysis of L929 MOUSE-BRAIN cells using IL-1β Polyclonal Antibody

Product Details

Uniprot No. P01584
Target Names IL1B
Alternative Names IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from the Internal region of Human IL-1β.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Preventive Effects of Pyungwi-san against Dextran Sulfate Sodium- and Clostridium difficile-Induced Inflammatory Bowel Disease in Mice. Yang M, et al,International Journal of Molecular Sciences,2019

Sample type: Colonic tissue lysates
Sample species: Mouse
Application: WB
Review: Western blot analysis showing the effect of PWS on the expression of interleukin (IL)-1 β , IL-10, and transforming growth factor (TGF)- β 1 in DSS + CD-treated mice. Representative blots from experiments for each protein are shown (upper panel). The density of the bands in each blot was quantified by performing densitometric analysis and was normalized to the amount of β -actin (lower panel). Data are represented as mean ± SD.

Imipenem toxicity in male reproductive organs as a result of inflammatory microenvironment and oxidative stress in germinal cells. Cherif B, et al,Toxicology,2019

Sample type: Testis tiusse
Sample species: Human
Application: IHC
Review: Bax, Bcl-2 and IL-1 beta expression analysis by immunohistochemical staining in testis of rat. Control; IM15, IM50 and IM100: groups treated with imipenem/cilastatin at respectively 15, 50
and 100 mg/kg; GM group treated with 50 mg/kg of gentamicin.
PMID: 30721722

Early Pattern of Epstein-Barr Virus Infection in Gastric Epithelial Cells by “Cell-in-cell”. Yue W, et al,Virologica Sinica,2019

Applications: WB
Review: The protein expression of IL-1b at the indicated time points post-infection.
PMID: 30911896

Target Data

Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Secreted
Protein Families IL-1 family
Tissue Specificity Expressed in activated monocytes/macrophages (at protein level).
Database Links

HGNC: 5992

OMIM: 147720

KEGG: hsa:3553

STRING: 9606.ENSP00000263341

UniGene: Hs.126256

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