MAPK9/MAPK10 (Ab-183) Antibody

Datasheet
Code CSB-PA117331
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P45984
Image
  • Western blot analysis of extracts from Hela and MCF cells using SAPK/JNK(Ab-183) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Ab-183) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Peptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK/JNK.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of total SAPK/JNK protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200
Relevance Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias JNK2
Immunogen Species Homo sapiens (Human)
Target Names MAPK9/MAPK10
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock
Subcellular Location Cytoplasm, Nucleus
Protein Families Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links

HGNC: 6886

OMIM: 602896

KEGG: hsa:5601

STRING: 9606.ENSP00000389338

UniGene: Hs.484371

Pathway cAMP signaling pathway
ErbB signaling pathway
MAPK signaling pathway
Wnt signaling pathway
Focal adhesion
TNF signaling pathway
Apoptosis
Autophagy
Necroptosis
Protein processing in endoplasmic reticulum
Ras signaling pathway
Fc epsilon RI signaling pathway
IL-17 signaling pathway
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation
Toll-like receptor signaling pathway
Adipocytokine signaling pathway
AGE-RAGE signaling pathway in diabetic complications
ER Stress pathway
FoxO signaling pathway
Insulin signaling pathway
Neurotrophin signaling pathway

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