Phospho-JUN (Thr91) Antibody

Code CSB-PA180546
Product Type Polyclonal Antibody
Size US$360
Uniprot No. P05412
  • Western blot analysis of extracts from 293 cells untreated or treated with UV using c-Jun(Phospho-Thr91) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr91) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunogen Peptide sequence around phosphorylation site of threonine 91 (T-T-T(p)-P-T) derived from Human c-Jun.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of c-Jun only when phosphorylated at threonine 91.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:100
Relevance Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Binetruy B, et al. (1991) Nature. 351: 122-127.
Smeal T, et al. (1991) Nature. 354:494-496.
Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias AH119; AP1; Jun A; c-Jun; p39
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names JUN
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells
Subcellular Location Nucleus
Protein Families BZIP family, Jun subfamily
Database Links

HGNC: 6204

OMIM: 165160

KEGG: hsa:3725

STRING: 9606.ENSP00000360266

UniGene: Hs.696684

Pathway cAMP signaling pathway
ErbB signaling pathway
Estrogen signaling pathway
MAPK signaling pathway
Wnt signaling pathway
Focal adhesion
TNF signaling pathway
B cell receptor signaling pathway
IL-17 signaling pathway
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Neurotrophin signaling pathway

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