Transcription factor AP-1 is a protein in humans that is encoded by JUN gene. Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
The following JUN reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
JUN Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA011972GA01HU | JUN Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA157903 | Phospho-JUN (Ser73) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA180546 | Phospho-JUN (Thr91) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA105550 | Phospho-JUN (Thr93) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA566229 | Phospho-JUN (Tyr170) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA258817 | Phospho-JUN (Thr239) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA205924 | Phospho-JUN (Ser243) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA832170 | JUN (Ab-63) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA040807 | JUN (Ab-73) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA438598 | JUN (Ab-91) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA928151 | JUN (Ab-93) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA587026 | JUN (Ab-170) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA925492 | JUN (Ab-239) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA074471 | JUN (Ab-243) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA185121 | Phospho-JUN (Ser63) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA570612 | JUN (Ab-63) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA790730 | JUN (Ab-243) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA000151 | Acetyl-JUN (K271) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA000471 | Phospho-JUN (S63) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA000473 | Phospho-JUN (T239) Antibody |
Human,Mouse,Rat | WB, IHC, IP, ELISA |
CSB-PA000475 | Phospho-JUN (T93) Antibody |
Human,Mouse,Rat | WB, IHC, IP, ELISA |
CSB-PA000886 | JUN Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA011034 | JUN Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA011294 | Phospho-JUN (S243) Antibody |
Human,Mouse,Rat | WB, IHC, IP, ELISA |
CSB-PA011295 | Phospho-JUN (T91) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA011296 | Phospho-JUN (Y170) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA011297 | Phospho-JUN (T231) Antibody |
Human,Mouse,Rat | IHC, IF, ELISA |
CSB-PA164448 | JUN Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-PA656958 | JUN Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-PA16929A0Rb | JUN Antibody |
Human, Mouse, Rat | ELISA, WB, IF |
CSB-PA16929B0Rb | JUN Antibody, HRP conjugated |
Human | ELISA |
CSB-PA16929D0Rb | JUN Antibody, Biotin conjugated |
Human | ELISA |
CSB-RA011972A63phHU | Phospho-JUN (S63) Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC, IF |
CSB-RA911286A0HU | JUN Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC |
JUN Antibodies for Avian sarcoma virus (strain 17)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA361514XA01ATE | JUN Antibody |
Avian sarcoma virus (strain 17) | ELISA, WB (ensure identification of antigen) |
JUN Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP011972RA CSB-EP011972RA CSB-BP011972RA CSB-MP011972RA CSB-EP011972RA-B |
Recombinant Rat Transcription factor AP-1 (Jun) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP011972CH CSB-EP011972CH CSB-BP011972CH CSB-MP011972CH CSB-EP011972CH-B |
Recombinant Chicken Transcription factor AP-1 (JUN) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP011972PI CSB-EP011972PI CSB-BP011972PI CSB-MP011972PI CSB-EP011972PI-B |
Recombinant Pig Transcription factor AP-1 (JUN) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP011972HU CSB-MP011972HU CSB-EP011972HU-B |
Recombinant Human Transcription factor AP-1 (JUN) |
Yeast Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP011972MO CSB-BP011972MO CSB-MP011972MO CSB-EP011972MO-B |
Recombinant Mouse Transcription factor AP-1 (Jun) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP011972BO CSB-EP011972BO CSB-BP011972BO CSB-MP011972BO CSB-EP011972BO-B |
Recombinant Bovine Transcription factor AP-1 (JUN) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Coturnix japonica (Japanese quail) (Coturnix coturnix japonica)
Code | Product Name | Source |
---|---|---|
CSB-YP011972DXJ CSB-EP011972DXJ CSB-BP011972DXJ CSB-MP011972DXJ CSB-EP011972DXJ-B |
Recombinant Coturnix coturnix japonica Transcription factor AP-1 (JUN) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Serinus canaria (Island canary) (Fringilla canaria)
Code | Product Name | Source |
---|---|---|
CSB-YP011972SYL CSB-EP011972SYL CSB-BP011972SYL CSB-MP011972SYL CSB-EP011972SYL-B |
Recombinant Serinus canaria Transcription factor AP-1 (JUN) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
JUN Proteins for Avian sarcoma virus (strain 17)
Code | Product Name | Source |
---|---|---|
CSB-YP361514ATE CSB-EP361514ATE CSB-BP361514ATE CSB-MP361514ATE CSB-EP361514ATE-B |
Recombinant Avian sarcoma virus Viral jun-transforming protein (JUN) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Jun was found as a novel oncoprotein encoded by a cellular insert in the genome of avian sarcoma virus 17 (ASV17), an acutely oncogenic retrovirus isolated from a spontaneous tumor in a chicken [1][2]. The human JUN gene encodes c-Jun highly similar to the viral protein, which interacts directly with specific target DNA sequences to regulate gene expression. c-Jun contains a docking site for the Jun N-terminal kinases (JNKs) which phosphorylate Serines 63 and 73, thereby enhancing its transcriptional potential and stability [3]. And c-Jun can form either homo- or heterodimers with members of the Jun family [4]. Fos–Jun and Jun–Jun dimers form the transcription factor AP‐1, a dimeric transcription factor complex [4]. Growth factors, hormones, and a variety of environmental stresses activate mitogen-activated protein kinase (MAPK) cascades that enhance Jun/AP-1 activity through phosphorylation, thereby regulating cell proliferation, differentiation, transformation, and/or apoptosis [4]. Functional data suggest that c‐Jun is not only a target for activation by many of the extracellular stimuli but also plays a role in mediating the cellular response. Ron Wisdom et al. revealed that in fibroblasts, c‐Jun is required for progression through the G1 phase of the cell cycle, and c‐Jun protects cells from UV‐induced apoptosis and cooperates with NF‐kappa B to prevent apoptosis induced by tumor necrosis factor-alpha (TNFα) [5]. Phosphorylation of c‐Jun on serines 63 and 73 by JNK is required for the UV protective effects, but not for the ability to promote cell proliferation [5]. c-jun transcription is autoregulated by its own product Jun [6], which may be a mechanism for prolonging the signals from extracellular stimuli. And this mechanism has biological significance for the activity of c-jun in cancer [6][7]. Also, c-jun activities can be regulated by the ERK pathway. Constitutively active ERK increases c-jun transcription and stability through CREB and GSK3. This results in activated c-jun and its downstream targets such as RACK1 and cyclin D1. RACK1 can enhance JNK activity, and activated JNK signaling subsequently exerts regulation on c-jun activity [8].
[1] Cavalieri F, Ruscio T, et al. Isolation of three new avian sarcoma viruses: ASV 9, ASV 17, and ASV 25 [J]. Virology 1985, 143(2): 680-683.
[2] Maki Y, Bos TJ, et al. Avian sarcoma virus 17 carries the jun oncogene [J]. Proc. Natl. Acad. Sci. USA 1987, 84: 2848-2852.
[3] Smeal T, Binetruy B, et al. Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73 [J]. 1991 Nature 354(6353): 494-496.
[4] Zenz R, Wagner EF. Jun signalling in the epidermis: From developmental defects to psoriasis and skin tumors [J]. Int J Biochem Cell Biol. 2006; 38(7):1043-9.
[5] Ron Wisdom, Randall S. Johnson, et al. c‐Jun regulates cell cycle progression and apoptosis by distinct mechanisms [J]. EMBO J (1999)18:188-197.
[6] Angel P, Hattori K, et al. The jun proto-oncogene is positively autoregulated by its product, Jun/AP-1 [J]. Cell. 1998, 55 (5): 875-85.
[7] Rotondo JC, Borghi A, et al. Association of Retinoic Acid Receptor β Gene With Onset and Progression of Lichen Sclerosus-Associated Vulvar Squamous Cell Carcinoma [J]. JAMA Dermatology. 2018, 154 (7): 819-823.
[8] Lopez-Bergami P, Huang C, et al. Rewired ERK-JNK signaling pathways in melanoma [J]. Cancer Cell. 2007, 11 (5): 447-60.