Phospho-NFKBIA (S32/S36) Antibody

Datasheet
Code CSB-PA000559
Size US$167
Image
  • Western Blot analysis of COS7 cells using Phospho-IκB-α (S32/S36) Polyclonal Antibody
  • Western Blot analysis of various cells using Phospho-IκB-α (S32/S36) Polyclonal Antibody

Product Details

Uniprot No. P25963
Target Names NFKBIA
Alternative Names NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Raised in Rabbit
Species Reactivity Human,Mouse,Rat,Monkey
Immunogen Synthesized peptide derived from Human IκB-α around the phosphorylation site of S32/S36.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

The novel thioredoxin reductase inhibitor A-Z2 triggers intrinsic apoptosis and shows efficacy in the treatment of acute myeloid leukemia. Dongdong Zhang, et al,Free Radical Biology and Medicine,2019

Sample type: AML cell lines
Sample species: Mouse
Application: WB
Review: AML cells were treated with 1 µM A-Z2 for 24 h, cell supernatants were harvested, cytochrome c level was measured by ELISA. Apoptosis (F) and cell cycle were analyzed after Molm-13 were treated with 1 µM A-Z2 for 24 h. AML-PC: AML patient primary cell.
PMID: 31730934

Genistein improves schistosomiasis liver granuloma and fibrosis via dampening NF-kB signaling in mice. Chunpeng Wan.et al,Parasitology Research,2017

Application: Mouse liver tissues
Sample type: Peripheral blood neutrophils
Review: Representative western blots for IκB, phosphor-IκB, p65, and phospho-p65 expressions in mouse liver tissues.
PMID: 28160073

Target Data

Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Involvement in disease Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID)
Subcellular Location Cytoplasm, Nucleus
Protein Families NF-kappa-B inhibitor family
Database Links

HGNC: 7797

OMIM: 164008

KEGG: hsa:4792

STRING: 9606.ENSP00000216797

UniGene: Hs.81328

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