Phospho-TGFBR1 (S165) Antibody

Datasheet
Code CSB-PA050248
Size US$167
Uniprot No. P36897
Image
  • Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFβ RI (S165) Polyclonal Antibody

  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunogen The antiserum was produced against synthesized peptide derived from human TGF beta Receptor I around the phosphorylation site of Ser165. AA range:131-180
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Tested Applications WB,IHC,ELISA;WB:1/500-1/2000.IHC:1/50-1/300.ELISA:1/5000
Form Liquid
Conjugate Non-conjugated
Storage Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Alias TGFBR1; ALK5; SKR4; TGF-beta receptor type-1; TGFR-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; ALK-5; ALK5; Serine/threonine-protein kinase receptor R4; SKR4; TGF-beta type I receptor; Transfor
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Target Names TGFBR1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.
Involvement in disease Loeys-Dietz syndrome 1 (LDS1); Multiple self-healing squamous epithelioma (MSSE)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Cell junction, tight junction, Cell surface, Membrane raft
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily
Tissue Specificity Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. Expressed in a variety of cancer cell lines (PubMed:25893292).
Database Links

HGNC: 11772

OMIM: 132800

KEGG: hsa:7046

STRING: 9606.ENSP00000364133

UniGene: Hs.494622

Pathway Hippo signaling pathway
MAPK signaling pathway
TGF-beta signaling pathway
Adherens junction
Cellular senescence
Osteoclast differentiation
Th17 cell differentiation
AGE-RAGE signaling pathway in diabetic complications
Apelin signaling pathway
FoxO signaling pathway
Endocytosis

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