Recombinant Arabidopsis thaliana Floral homeotic protein APETALA 1 (AP1)

Code CSB-YP339569DOA
MSDS
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Source Yeast
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Code CSB-EP339569DOA
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Source E.coli
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Code CSB-EP339569DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339569DOA
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Source Baculovirus
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Code CSB-MP339569DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AP1
Uniprot No.
Alternative Names
AP1; AGL7; At1g69120; F4N2.9; Floral homeotic protein APETALA 1; Agamous-like MADS-box protein AGL7
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-256
Target Protein Sequence
MGRGRVQLKR IENKINRQVT FSKRRAGLLK KAHEISVLCD AEVALVVFSH KGKLFEYSTD SCMEKILERY ERYSYAERQL IAPESDVNTN WSMEYNRLKA KIELLERNQR HYLGEDLQAM SPKELQNLEQ QLDTALKHIR TRKNQLMYES INELQKKEKA IQEQNSMLSK QIKEREKILR AQQEQWDQQN QGHNMPPPLP PQQHQIQHPY MLSHQPSPFL NMGGLYQEDD PMAMRRNDLE LTLEPVYNCN LGCFAA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that promotes early floral meristem identity in synergy with LEAFY. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Is indispensable for normal development of sepals and petals in flowers. Regulates positively the B class homeotic proteins APETALA3 and PISTILLATA with the cooperation of LEAFY and UFO. Interacts with SEPALLATA3 or AP3/PI heterodimer to form complexes that could be involved in genes regulation during floral meristem development. Regulates positively AGAMOUS in cooperation with LEAFY. Displays a redundant function with CAULIFLOWER in the up-regulation of LEAFY. Together with AGL24 and SVP, controls the identity of the floral meristem and regulates expression of class B, C and E genes. Represses flowering time genes AGL24, SVP and SOC1 in emerging floral meristems.
Gene References into Functions
  1. APETALA1 establishes determinate floral meristem through regulating cytokinins homeostasis in Arabidopsis. PMID: 26359644
  2. suppression of cytokinin biosynthesis and activation of cytokinin degradation mediates AP1 function in establishing determinate floral meristems PMID: 24753595
  3. Co-expression analysis identifies CRC and AP1 the regulator of Arabidopsis fatty acid biosynthesis. PMID: 22676405
  4. Different action of the APETALA1 gene on the development of reproductive organs in flowers of the abruptus mutant of Arabidopsis thaliana PMID: 21950056
  5. results suggest distinct functions of AP1 during the initiation of flower development PMID: 20360106
  6. The floral homeotic PISTILLATA (PI) protein and its interacting partner APETALA3 directly act, in combination with other factors, to restrict the expression of AP1 during early stages of floral development. PMID: 16640596
  7. AP1, AGL24 and SVP redundantly control floral meristem identity. PMID: 18694458
  8. Angiostatin K1-3 induced E-selectin expression via AP1 and Ets-1 binding to the proximal E-selectin promoter (-356/+1), which was positively mediated by JNK activation. PMID: 18761727

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in young flower primordia, later becomes localized to sepals and petals.
Database Links

KEGG: ath:AT1G69120

STRING: 3702.AT1G69120.1

UniGene: At.10200

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