Recombinant Human TNF receptor-associated factor 3(TRAF3)

Code CSB-YP024148HU
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Source Yeast
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Code CSB-EP024148HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP024148HU
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Source Baculovirus
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Code CSB-MP024148HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names TRAF3
Uniprot No. Q13114
Alternative Names CAP 1; CAP-1; CAP1 ; CD40 associated protein 1 ; CD40 binding protein ; CD40 bp; CD40 receptor associated factor 1; CD40 receptor-associated factor 1; CD40-binding protein; CD40BP; CRAF 1; CRAF1; IIAE5; LAP 1; LAP1; LMP1 associated protein ; LMP1-associated protein 1; MIPT3; TNF receptor associated factor 3 ; TNF receptor-associated factor 3; TRAF 3; Traf3; TRAF3_HUMAN
Species Homo sapiens (Human)
Expression Region 1-568
Target Protein Sequence MESSKKMDSP GALQTNPPLK LHTDRSAGTP VFVPEQGGYK EKFVKTVEDK YKCEKCHLVL CSPKQTECGH RFCESCMAAL LSSSSPKCTA CQESIVKDKV FKDNCCKREI LALQIYCRNE SRGCAEQLML GHLLVHLKND CHFEELPCVR PDCKEKVLRK DLRDHVEKAC KYREATCSHC KSQVPMIALQ KHEDTDCPCV VVSCPHKCSV QTLLRSELSA HLSECVNAPS TCSFKRYGCV FQGTNQQIKA HEASSAVQHV NLLKEWSNSL EKKVSLLQNE SVEKNKSIQS LHNQICSFEI EIERQKEMLR NNESKILHLQ RVIDSQAEKL KELDKEIRPF RQNWEEADSM KSSVESLQNR VTELESVDKS AGQVARNTGL LESQLSRHDQ MLSVHDIRLA DMDLRFQVLE TASYNGVLIW KIRDYKRRKQ EAVMGKTLSL YSQPFYTGYF GYKMCARVYL NGDGMGKGTH LSLFFVIMRG EYDALLPWPF KQKVTLMLMD QGSSRRHLGD AFKPDPNSSS FKKPTGEMNI ASGCPVFVAQ TVLENGTYIK DDTIFIKVIV DTSDLPDP
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
Gene References into Functions
  1. A TRAF3-NIK axis differentially regulates viral DNA vs RNA pathways in innate immune signaling. PMID: 30018345
  2. Association between the rs1883832 and rs3765459 CD40 gene polymorphism and susceptibility to cervical cancer in a subset of Malaysian population. PMID: 28181356
  3. Study demonstrated that TRAF3, as a novel RIP2 binding partner, was downregulated in glioma tissues and functionally was a negative regulator involved in RIP2induced glioma cell growth. PMID: 29693188
  4. Data show that TNF receptor-associated factor 3 (TRAF3) autophagy is driven by RAS and results in activation of transcription factor RelB (RELB). PMID: 29146913
  5. MicroRNA-214 regulates immunity-related genes in bovine mammary epithelial cells by targeting NFATc3 and TRA PMID: 28627449
  6. These results indicate that TRAF3 deficiency suffices to metabolically reprogram B cells, a finding that improves our understanding of the role of TRAF3 as a tumor suppressor, and suggests potential therapeutic strategies. PMID: 27752131
  7. Viral proteins aim to subvert TRAF3 antiviral action. PMID: 27208423
  8. Mechanistic studies showed that HACE1 exerts its inhibitory role on virus-induced signaling by disrupting the MAVS-TRAF3 complex. PMID: 27213432
  9. An important B cell-specific role for TNFR-associated factor 3 is the inhibition of homeostatic survival, directly relevant to the common occurrence of TNFR-associated factor 3 mutations in human B cell malignancies. Review. PMID: 27154354
  10. The current investigations identified a subset of HPV-positive HNSCCs with mutations in the genes TRAF3 (tumor necrosis factor receptor-associated factor 3) and CYLD (cylindromatosis lysine 63 deubiquitinase). Defects in TRAF3 and CYLD correlated with the activation of transcriptional factor nuclear factor kappaB, episomal HPV status of tumors, and improved patient survival. PMID: 28295222
  11. NDR1 interacts with TRAF3 and interferes with the association of TRAF3 and IL-17R, resulting in increased formation of the activation complex IL-17R-Act1, which is required for the downstream signaling and production of pro-inflammatory factors PMID: 28219902
  12. Data suggest that UBR5 down-regulates levels of TRAF3, a key component of Toll-like receptor signaling, via the miRNA pathway; p90RSK is an upstream regulator of UBR5; p90RSK phosphorylates UBR5 as required for translational repression of TRAF3 mRNA. (UBR5 = ubiquitin protein ligase E3 component n-recognin 5 protein; TRAF3 = TNF receptor-associated factor 3; p90RSK = 90 kDa ribosomal protein S6 kinase) PMID: 28559278
  13. The GA genotype and GA+AA genotype of TRAF3 rs12147254 were found to increase the risk of coronary heart disease among T2DM patients. the GACGAC haplotype in TRAF3 had a protective effect on T2DM micro-macrovascular complications. PMID: 28479387
  14. These data suggest an interplay between CELF2 and hnRNP C as the mechanistic basis for activation-dependent alternative splicing of TRAF3 exon 8. PMID: 28031331
  15. DDX3 directly regulates TRAF3 ubiquitination and acts as a scaffold to co-ordinate assembly of signaling complexes downstream from MAVS. PMID: 27980081
  16. The NleB effector limited host IFN-beta production by inhibiting Lys(63)-linked ubiquitination of TNF receptor-associated factor 3 (TRAF3). Inhibition was dependent on the glycosyltransferase activity of NleB. PMID: 27387501
  17. TRAF3 epigenetics may significantly contribute to the variability of clopidogrel response and recurrence of ischemic events in patients with stroke. PMID: 27026631
  18. Taken all together, Helicobacter pylori toxin Tip-alpha might activate NF-kappaB to promote inflammation and carcinogenesis by inhibiting miR-3178 expression, which directly targets TRAF3, during Helicobacter pylori infection in gastric mucosal epithelial cells. PMID: 27493095
  19. PLK1 overexpression in prostate epithelial cells triggers oncogenic transformation. The signaling mechanisms underlying the observed cellular effects of PLK1 involve direct PLK1-dependent phosphorylation of CRAF with subsequent stimulation of the MEK1/2-ERK1/2-Fra1-ZEB1/2 signaling pathway. PMID: 27003818
  20. These findings suggest that RNF166 positively regulates RNA virus-triggered IFN-beta production by enhancing the ubiquitination of TRAF3 and TRAF6. PMID: 26456228
  21. Findings establish CK1varepsilon as a regulator of antiviral innate immune responses and indicate a novel mechanism of immunoregulation that involves CK1varepsilon-mediated phosphorylation of TRAF3. PMID: 26928339
  22. findings identify TRAF3 and PTPN22 as inhibitors of IL-6R signaling in B cells and reveal a previously uncharacterized role for TRAF3 in the regulation of plasma cell differentiation PMID: 26329582
  23. TRAF3 regulates B-cell survival via inhibition of CREB stability PMID: 26755589
  24. ESR1 directly interacts with TRAF3 and promotes K48-linked proteasomal degradation of TRAF3. PMID: 26186972
  25. these findings demonstrate that WDR82 is a negative regulator of virus-triggered type I IFNs pathway through mediating TRAF3 polyubiquitination status and stability on mitochondria. PMID: 26519536
  26. RIG-I-like receptor mediated antiviral innate immune responses in the lower respiratory tract involves TRAF3 and TRAF5 signaling. PMID: 26454171
  27. TRAF3 regulates signaling to T cells not only through costimulatory members of the TNFR superfamily, but also through the T cell receptor complex, and cytokine receptors. PMID: 25723057
  28. Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a positive regulator of pathological cardiac hypertrophy in mice and humans. PMID: 26034202
  29. Focal genetic loss of TRAF3 is recurrent in human diffuse large B-cell lymphoma. PMID: 25468570
  30. The role of TRAF3 and NIK in T cell malignancies is clarified. Results indicate that TRAF3 differentially governs the growth of B and T cell cancers. PMID: 24739416
  31. It has become increasingly evident that the roles and functions of TRAF3 are highly context-dependent. TRAF3 can serve distinct roles for different receptors in the same cell, and also has highly cell-type-dependent functions. PMID: 24433987
  32. After viral infection, HSCARG interacted with tumor necrosis receptor-associated factor 3 (TRAF3) and inhibited its ubiquitination by promoting the recruitment of OTUB1 to TRAF3. PMID: 24763515
  33. SUMOylation is required for optimal TRAF3 signaling capacity. PMID: 24260396
  34. a mechanistic basis for Traf3 alternative splicing and ncNFkappaB activation in contributing to T cell-dependent adaptive immunity. PMID: 24671418
  35. These findings demonstrate that herpes simplex virus 1 ubiquitin-specific protease UL36 removes polyubiquitin chains on TRAF3 and counteracts the IFN-beta pathway. PMID: 23986588
  36. noncanonical NF-kappaB activation by Tio depends on a distinct sequence motif that directly recruits tumor necrosis factor receptor-associated factor 3 (TRAF3). PMID: 23612708
  37. TRAF3 and TRAF5 are overexpressed in inflammatory bowel disease. Although the endoscopic appearance can be normal, TRAF3 and TRAF5 pre-activation can be detected in non-inflamed colonic segments PMID: 23329887
  38. In an attempt to explore TRAF3 binding partners that may be involved in TRAF3-regulated signaling, a yeast two-hybrid screen of a human spleen cDNA library was undertaken. RIP2 was identified as a TRAF3 binding partner. PMID: 23333941
  39. Levels of TRAF3 degradation correlate with number of cells infected with Chlamydia pneumoniae, because more TRAF3 is detected at a lower multiplicity of C. pneumoniae infection. PMID: 23303668
  40. model proposed where dsDNA and dsRNA sensing induces the formation of membrane-bound compartments originating from the Golgi, which mediate the dynamic association of TRAF3 with MAVS leading to an optimal induction of innate immune responses PMID: 22792062
  41. Ubiquitination of NEMO negatively regulates the interferon antiviral response through disruption of the MAVS-TRAF3 complex. PMID: 22901541
  42. A novel microRNA-32 mediated mechanism regulates TRAF3 in human microglial cells exposed to human immunodeficiency virus (HIV)-1 Tat protein. PMID: 22709905
  43. TRAF3 functioned as ubiquitin E3 ligase for calcineurin and promoted its degradation. PMID: 22715070
  44. Genetic lesions of the TRAF3 and MAP3K14 genes in classical Hodgkin lymphoma. PMID: 22469134
  45. MIP-T3 is unique among cellular inhibitors of innate immunity because it appears to affect TRAF3 ubiquitination only slightly, but is capable of preventing TRAF3 from engaging downstream transducers and effectors. PMID: 22079989
  46. TRAF3 participates in a number of the regulatory post-translational modifications involving ubiquitin that are important in various signaling pathways. PMID: 22017431
  47. Data show that IKBalpha, NFKB2, and TRAF3 gene polymorphisms play a role in the development of multiple myeloma and in the response to bortezomib therapy. PMID: 21228035
  48. Studies indicate that TRAF3 plays a highly versatile role of in immunity-related signal transduction. PMID: 21660053
  49. Lack of TRAF3 expression might be one of the reasons for the aberrant expression of the unclassical NF-kappaB activity in Hodgkin's lymphoma cells. PMID: 19954665
  50. In contrast to TRAF3 transgenic mice lacking TRAF3 in B cells, T cell TRAF3-deficient mice exhibit defective IgG1 responses to T-dependent antigen, as well as impaired T cell-mediated immunity to infection with Listeria monocytogenes. PMID: 21084666

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Involvement in disease Herpes simplex encephalitis 3 (HSE3)
Subcellular Location Cytoplasm, Endosome, Mitochondrion
Protein Families TNF receptor-associated factor family, A subfamily
Database Links

HGNC: 12033

OMIM: 601896

KEGG: hsa:7187

STRING: 9606.ENSP00000376500

UniGene: Hs.510528

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