Product Details

Purity

>85% (SDS-PAGE)

Target Names

Cdk6

Uniprot No.

Q64261

Alternative Names

Cdk6; Cdkn6; Crk2Cyclin-dependent kinase 6; EC 2.7.11.22; CR2 protein kinase; CRK2; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE

Species

Mus musculus (Mouse)

Expression Region

1-326

Target Protein Sequence

MEKDSLSRAD QQYECVAEIG EGAYGKVFKA RDLKNGGRFV ALKRVRVQTS EEGMPLSTIR EVAVLRHLET FEHPNVVRLF DVCTVSRTDR ETKLTLVFEH VDQDLTTYLD KVPEPGVPTE TIKDMMFQLL RGLDFLHSHR VVHRDLKPQN ILVTSSGQIK LADFGLARIY SFQMALTSVV VTLWYRAPEV LLQSSYATPV DLWSVGCIFA EMFRRKPLFR GSSDVDQLGK ILDIIGLPGE EDWPRDVALP RQAFHSKSAQ PIEKFVTDID ELGKDLLLKC LTFNPAKRIS AYGALNHPYF QDLERYKDNL NSHLPSNQST SELNTA

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.

Gene References into Functions

The downregulation of Cdkn1a and the upregulation of Cdk6. PMID: 29357734
data indicate that Gli3 controls the onset of cortical neurogenesis by determining the levels of Cdk6 expression, thereby regulating neuronal output and cortical size. PMID: 30093555
Cdk6 interacts with a number of proteins involved in cytoskeleton organization. Cdk6 regulates the transcription of a panel of genes involved in actin (de-)polymerization. PMID: 28255017
CDK6-mediated suppression of CD25 is required for initiation of T-ALL by activated Notch1. . Pharmacologic inhibition of CDK6 kinase induces CD25 and RUNX1 expression, cell cycle arrest and apoptosis in mouse and human T-ALL. PMID: 26707936
Fbxo7-deficient immature thymocytes failed to undergo expansion in the thymus due to a lack of Cdk6 activity, while mature T cells showed enhanced proliferative capacity upon T-cell receptor engagement due to reduced p27 levels. These studies reveal differential cell cycle regulation by Fbxo7 at different stages in T-cell development. PMID: 27915416
Taken together, our results suggested that microRNA-33 enhanced the replicative senescence of MEFs potentially by suppressing CDK6 expression. PMID: 27059142
Data show that E11/glycoprotein 38(GP38) was up-regulated upon SEMA3A stimulation, and cyclin-dependent kinase 6 (CDK6) was down-regulated in a time-dependent manner. PMID: 27107080
Notch3 transcription and growth of lymphoma cells was dependent on CDK6. PMID: 25483078
CDK6 is an important regulator of stem cell activation and an essential component of a transcriptional complex that suppresses Egr1 in hematopoietic and leukemic stem cell activation PMID: 25342715
Cdk6 deficiency prevents the expansion of neuronally committed precursors by lengthening G(1) phase duration, reducing concomitantly the production of newborn neurons PMID: 21319271
a mechanism for LPS signaling which involves a decrease in miR-107 leading to an increase in CDK6 PMID: 21628465
an important role for CDK6 kinase activity in thymocyte development that operates partially through modulating Notch target gene expression. PMID: 21508411
we identify CDK6 as relevant and critical target of AP-1-regulated DNA methylation on BCR-ABL-induced transformation, thereby accelerating leukemogenesis PMID: 21300982
These studies provide in vivo evidence that CDK4 and CDK6 play a similar role as a mediator of keratinocyte proliferation but differ in apoptosis activation and skin tumor development. PMID: 21224071
This study propose that chronic inflammation decreases spinal microglial/macrophage GRK2, which prevents silencing of microglia/macrophage activity and thereby contributes to prolonged hyperalgesia. PMID: 20609517
Data show that IO treatment provoked an increase in CDK6, Cyclin E, p27kip1, and p21waf1 expression, and after 6BIO treatment, the expression of most of these proteins decreased. PMID: 19860623
PU.1 coordinates proliferation and differentiation in immature erythroid cells by inhibiting the GATA-1-mediated gene expression program and also by regulating expression of cdk6 and genes that control progression through the G(1) phase of the cell cycle PMID: 19955566
Evidence for coordinated interaction of cyclin D3 with p21 and cdk6 in directing the development of uterine stromal cell decidualization and polyploidy during implantation PMID: 11804782
p18(INK4C) inhibits CD40-signaled cell cycle progression by preferential inhibition CDK6 PMID: 12196289
adapter Crk-II is constitutively associated with C3G, a GDP to GTP exchange factor for several small GTP-binding proteins PMID: 12215429
CDK6 blocks differentiation, coupling cell proliferation to the block to differentiation in leukemic cells PMID: 12833137
expression of cdk6 resulted in a distinct and rapid morphology change from multipolar to bipolar PMID: 12861051
oocytes regulators amounts and localization undergo dramatic changes as the oocytes grow, meiotically mature, and transit into embryogenesis and CDK4, CDK6, cyclin D2, and p27 appear in unusual, most likely posttranslationally modified, forms. PMID: 13679319
Cdk6 is a critical regulator of BMP-2-induced osteoblast differentiation and its Smads-mediated down-regulation is essential for efficient osteoblast differentiation PMID: 15254224
Cdk6 may be a regulator of chondrocyte differentiation and that its p38-mediated downregulation is involved in the efficient differentiation PMID: 15795936
cdk6 expression in primary astrocytes favored the expression of progenitor cell markers PMID: 16767702
Results show a critical requirement for CDK6 in Notch/Akt-dependent T-cell development and tumorigenesis. PMID: 19155308
These findings identified Znhit1 as a CDK6 regulator that plays an important role in cell proliferation. PMID: 19501046
A majority of cdk6 is localized to the cytoplasm with concentrations of cdk6 in the edges of the cytoplasm and in the cytoplasmic extensions of cells. PMID: 19667758

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Subcellular Location

Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily

Tissue Specificity

Expressed in subgranular zone (SGZ) of the hippocampal dentate gyrus (DG) and the subventricular zone (SVZ) of the lateral ventricles whose neural precursor cells (NPC) give rise to dentate granule neurons and olfactory bulb (OB) interneurons, respectivel

Database Links

KEGG: mmu:12571

STRING: 10090.ENSMUSP00000037925

UniGene: Mm.31672

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Recombinant Mouse Cyclin-dependent kinase 6 (Cdk6)

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