Code | CSB-RA697694A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The CDK4 recombinant monoclonal antibody is created using protein technology and DNA recombinant technology. Initially, mice are immunized with a synthesized peptide from human CDK4, and after a certain period of time, the spleen cells are removed under aseptic conditions. The total RNA of spleen cells is extracted and converted into cDNA by RNA reverse transcription. The cDNA is used as a template for PCR amplification of the CDK4 antibody gene. The obtained CDK4 antibody gene is then introduced into a vector, which is transfected into host cells for culture. The CDK4 recombinant monoclonal antibody is purified from the supernatant of cell culture through affinity chromatography and has been thoroughly verified for its use in ELISA and WB experiments in detecting human CDK4 protein.
CDK4 is a protein that functions as a serine/threonine kinase. It plays a crucial role in cell cycle progression by regulating the G1/S transition. Once activated, the CDK4-cyclin D complex phosphorylates and inactivates the retinoblastoma protein (Rb). Phosphorylation of Rb releases E2F, which then activates the expression of genes required for DNA replication and cell division. Dysregulation of CDK4 activity has been implicated in the development of various types of cancer.
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