Product Details

Uniprot No.

P35222

Target Names

CTNNB1

Alternative Names

Catenin beta-1 (Beta-catenin), CTNNB1, CTNNB

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human beta Catenin

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Beta-catenin, encoded by the CTNNB1 gene, serves as a central hub in cellular biology, functioning both as a structural component of adherens junctions and as the key effector of canonical Wnt signaling. This dual role makes it indispensable for understanding processes ranging from embryonic development and stem cell maintenance to tumor progression, where aberrant beta-catenin accumulation drives oncogenic transcription programs across multiple cancer types.

This recombinant monoclonal antibody, clone 5E1, offers the reproducibility that demanding research requires. Generated against a synthetic peptide derived from human beta-catenin and produced using recombinant technology, it provides sequence-defined consistency that eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies. For researchers conducting longitudinal studies or multi-site collaborations, this translates to reliable, comparable results across experiments.

The antibody has been validated for immunohistochemistry on formalin-fixed, paraffin-embedded human tissues, with demonstrated performance in both breast cancer and liver cancer specimens at 1:100 dilution using citrate buffer antigen retrieval. These validation studies confirm its utility for examining beta-catenin localization patterns, whether assessing membrane-associated pools at cell-cell contacts or detecting nuclear accumulation indicative of active Wnt signaling. The antibody is also validated for ELISA applications, providing flexibility for quantitative detection workflows.

Whether investigating Wnt pathway dysregulation in oncology research, examining cell adhesion dynamics in cardiovascular studies, characterizing stem cell populations, or exploring beta-catenin's emerging roles in neuronal function, this antibody delivers the specificity and consistency needed for meaningful experimental outcomes in human tissue and cell-based systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling.

Gene References into Functions

CXC chemokine ligand 9 promotes the progression of diffuse large B-cell lymphoma in a beta-catenin-dependent manner. PMID: 30130730
Results suggest that epigenetic regulation of CTNNB1 may serve as a novel avenue to block colon cancer cell migration and invasion. PMID: 29923144
The results demonstrated that 2HF could inhibit EMT, and cell migration and invasion through the Wnt/bcatenin signaling pathway by suppressing GSK3b phosphorylation, betacatenin expression and transactivation. PMID: 30226607
Collectively, these studies suggested the cellular transcription factor beta-catenin stimulates productive herpes simplex virus 1infection, in part because VP16 enhances beta-catenin dependent transcription. PMID: 30077727
CTNNB1 mutations may be more related to tumorigenesis ( aldosterone-producing adenoma) rather than excessive aldosterone production PMID: 28102204
CTNNB1 mutations were found in 60% of Basal cell adenoma but not in basal cell adenocarcinoma. None of the tested cases had PIK3CA mutations. CTNNB1 mutation trended to be more common in those cases having a predominant tubular or tubulotrabecular patterns. PMID: 29224720
Data reveal that post-translational modifications of beta-catenin in the ubiquitin-proteasome pathway yield a truncated beta-catenin molecule containing a serine 552-phosphorylated core region without N and C termini. This proteolytic processing of beta-catenin is required for binding with TCF4 and subsequent transcriptional activation. PMID: 29330435
Results identify CTNNB1 as a Girdin-interacting protein. Girdin-depleted skin cancer cells displayed scattering and impaired E-cadherin-specific cell-cell adhesion. PMID: 30194792
the dysregulation of TET2/E-cadherin/beta-catenin regulatory loop is a critical oncogenic event in HCC progression PMID: 29331390
High CTNNB1 expression is associated with bladder cancer progression. PMID: 30015971
It has been found that miR-27a-3p modulated the Wnt/beta-catenin signaling pathway to promote epithelial-mesenchymal transition in oral squamous carcinoma stem cells by down-regulating SFRP1. PMID: 28425477
Beta-catenin pathway is activated by CBX8 in in hepatocellular carcinoma. PMID: 29066512
our data provide a novel evidence for the biological and clinical significance of SPAG5 as a potential biomarker, and we demonstrate that SPAG5-b-catenin-SCARA5 might be a novel pathway involved in hepatocellular carcinoma progression. PMID: 30249289
Results show that hypoxia enhanced nuclear accumulation and transcriptional activity of beta-catenin which promotes expression of EMT-related genes and eventually contributes to the metastatic process in lung cancer cells. PMID: 30396950
This study demonstrates that FOXC1 induces cancer stem cells (CSCs)-like properties in non-small cell lung cancer (NSCLC) by promoting beta-catenin expression. The findings indicate that FOXC1 is a potential molecular target for anti-CSC-based therapies in NSCLC PMID: 30189871
High TBL1XR1 expression indicates poor disease-free survival of stage I-III colorectal cancer patients; beta-catenin signaling is critical for TBL1XR1-mediated colorectal cancer cells oncogenicity. PMID: 28295012
Taking together, these results suggest that Wnt/beta-catenin signal pathway activation-dependent up-regulation of syncytin-1 contributes to the pro-inflammatory factor TNF-alpha-enhanced fusion between oral squamous cell carcinoma cells and endothelial cells. PMID: 28112190
The disassociation of the beta-catenin/E-cadherin complex in the osteoblast membrane under stretch loading and the subsequent translocation of beta-catenin into the nucleus may be an intrinsic mechanical signal transduction mechanism. PMID: 29901167
Aberrant CTNNB1 expression was seen in a substantial proportion of our hepatocellular carcinoma (HCC) cases. CTNNB1-positive HCC was associated with normal AFP levels, unicentric tumors, well-differentiated histology, and an unfavorable outcome. PMID: 30082549
Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-beta-catenin signaling. PMID: 29901121
High CTNNB1 expression is associated with the recurrece of Adamantinomatous Craniopharyngiomas. PMID: 29625497
High CTNNB1 expression is associated with uterine fibroids. PMID: 29066531
The nucleus and/or cytoplasm expression of beta-catenin was associated with tumor progression and correlated overall survival of patients with ovarian cancer (OC). beta-catenin may be a possible potential prognostic biomarker for the patients with OC. [review] PMID: 30103006
In the two wild type (WT) cases, two novel alterations were detected: a complex deletion of APC and a pathogenic mutation of LAMTOR2. Focusing on WT DT subtype, deep sequencing of CTNNB1, APC and LAMTOR2 was conducted on a retrospective series of 11 WT DT using a targeted approach PMID: 29901254
DLX1 interacted with beta-catenin and enhanced the interaction between beta-catenin and TCF4 T-cell factor PMID: 29317218
Nuclear beta-catenin immunoreactivity with appropriate criteria may be helpful to distinguish basal cell adenocarcinoma (BCAC) from histologically similar tumors. However, a minor subset of adenoid cystic carcinoma (ACC) with nuclear beta-catenin expression require careful diagnosis. PMID: 29496310
High CTNNB1 expression is associated with metastasis in cholangiocarcinoma. PMID: 30193944
beta;-catenin directly interacts with the Cx43 carboxyl-terminal domain. PMID: 29882937
This study showed that beta-catenin expression was the most evident in the nucleus rather than in cytoplasm. PMID: 29297710
Nuclear beta-catenin accumulation in non-mitotic glioblastoma cells is due to a feed forward mechanism between DOCK4 and beta-catenin. PMID: 28925399
Study found that HIF1alpha overexpression led to an enhanced betacatenin nuclear translocation, while betacatenin silencing inhibited betacatenin nuclear translocation. The enhanced betacatenin nuclear translocation induced resulted in an enhanced cell proliferation and cell invasion, an altered cell cycle distribution, decreased apoptosis, and improved nonhomologous end joining repair under normal and irradiation cond... PMID: 29658569
our results demonstrated that miR-188 inhibits glioma cell proliferation by targeting beta-catenin PMID: 29268818
Marked upregulation of beta-catenin and its downstream targets effectively enhanced hepatosphere formation, with an associated induction of CD133, OCT4 and Sox2 expression and also caused an significant enhancement of HCC proliferation PMID: 29792038
Wnt/beta-catenin signaling pathway may play a significant role in the pathogenesis of preeclampsia by regulating the invasion and proliferation of trophoblast. PMID: 29603045
Associations between environmental variants together with single nucleotide polymorphisms (SNPs) of beta-catenin (ctnnb1) and lung cancer risk were analyzed using a logistic regression model. PMID: 29562493
that CTNNB1 is overexpressed and confers a poor prognosis in acute myeloid leukemia PMID: 29496308
High CTNNB1 expression is associated with cisplatin-resistance in non-small cell lung cancer. PMID: 30009824
beta-catenin immunopositivity is seen in majority of cases of sinonasal sarcoma PMID: 29566950
For the first time, we demonstrated that rather than excluding lymphocytes infiltration as reported in mela-noma, high levels of TILs were associated with beta-catenin overexpression in BC. PMID: 29286921
Study shows that apigenin-induced lysosomal degradation of beta-catenin in Wnt/beta-catenin signaling. PMID: 28337019
Used CRISPR-Cas9 technology to study effect of knockout of catenin beta 1 (CTNNB1) on cell behavior and signal pathways in HEK293 cells. Results showed knockout of CTNNB1 effected Wnt/beta-catenin signaling pathway and suppressed adhesion and proliferation of HEK 293T cells. PMID: 29249062
our results also revealed that lncRNA SNHG20 knockdown inhibited Wnt/b catenin signaling activity by suppressing beta-catenin expression and reversing the downstream target gene expression. Taken together, lncRNA SNHG20 plays an pivotal role in ovarian cancer progression by regulating Wnt/b-catenin signaling PMID: 29101241
Wnt3A regulates the expression of 1,136 genes, of which 662 are upregulated and 474 are downregulated in CCD-18Co cells. A set of genes encoding inhibitors of the Wnt/beta-catenin pathway stand out among those induced by Wnt3A, which suggests that there is a feedback inhibitory mechanism. PMID: 29044515
The aim of our study was to analyze the immunohistochemical expression of beta-catenin, E-cadherin and Snail, depending on clinico-morphological aspects of the laryngeal squamous cell carcinomas. Results revealed variable E-cadherin, beta-catenin and Snail expression, depending on differentiation degree and tumor stage. PMID: 29250652
In this study we showed that the activation of Wnt/beta-catenin pathway culminates in the upregulation of MGAT1 enzyme both at transcriptional and post-transcriptional levels. We also showed that overexpression of the beta-catenin gene (CTNNB1) increased the promoter activity of MGAT1. PMID: 29310626
CTNNB1 mutation is associated with acquired resistance to KIT inhibitor in metastatic melanoma. PMID: 28421416
three CTNNB1 SNPs were suggested to have the potential to be novel biomarkers for risk prediction of cancer in overall population or some specific subgroups. [Review] PMID: 28963373
A CTNNB1 exon 3 mutation restricted to the areas exhibiting both positive glutamine synthetase (GS) and C-reactive protein (CRP) expression, whereas wild-type CTNNB1 was found in areas showing only CRP staining. These two cases illustrate focal beta-catenin activation that can occur within Inflammatory hepatocellular adenoma (IHCAs). PMID: 28618047
Results show that E-cadherin/beta-catenin complex is disrupted by ICAT promoting epithelial-mesenchymal transition of cervical cancer cells. PMID: 29048651
Toosendanin administration inhibited growth and liver metastasis of orthotopically implanted SGC7901 tumors in vivo through miR200amediated beta-catenin pathway. Our data suggest that Toosendanin may suppress oncogenic phenotypes of human GC cells partly via miR200a/beta-catenin axis. Hence, Toosendanin may have a promising chemotherapeutic activity for GC therapy. PMID: 29048657

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Involvement in disease

Colorectal cancer (CRC); Pilomatrixoma (PTR); Medulloblastoma (MDB); Ovarian cancer (OC); Mesothelioma, malignant (MESOM); Mental retardation, autosomal dominant 19 (MRD19); Vitreoretinopathy, exudative 7 (EVR7)

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse. Cytoplasm, cytoskeleton, cilium basal body.

Protein Families

Beta-catenin family

Tissue Specificity

Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level).

Database Links

HGNC: 2514

OMIM: 114500

KEGG: hsa:1499

STRING: 9606.ENSP00000344456

UniGene: Hs.476018

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Code	CSB-RA155605A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA155605A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA155605A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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CTNNB1 Recombinant Monoclonal Antibody

Product Details

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Target Background