Human Catenin beta-1 (CTNNB1/CTNNB/OK/SW-cl.35/PRO2286) ELISA kit

Code CSB-E08963h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P35222
Lead Time 3-5 working days
Abbreviation CTNNB1
Protein Biological Process 1 Cell Adhesion
Target Name catenin (cadherin-associated protein), beta 1, 88kDa
Alias CTNNB, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000209288|beta-catenin
Species Homo sapiens (Human)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 2514
RGD 70487
MGI 88276
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CTNNB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-98  
1:2 Average % 93  
Range % 89-95  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 96  
Range % 86-102  
Recovery
The recovery of human CTNNB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-104  
EDTA plasma (n=4) 89 85-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.199 2.277 2.238 2.075  
500 1.743 1.663 1.703 1.540  
250 1.179 1.157 1.168 1.005  
125 0.794 0.800 0.797 0.634  
62.5 0.472 0.460 0.466 0.303  
31.2 0.327 0.333 0.330 0.167  
15.6 0.232 0.226 0.229 0.066  
0 0.163 0.162 0.163    
References
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Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin
Involvement in disease Colorectal cancer (CRC); Pilomatrixoma (PTR); Medulloblastoma (MDB); Ovarian cancer (OC); Mesothelioma, malignant (MESOM); Mental retardation, autosomal dominant 19 (MRD19); Vitreoretinopathy, exudative 7 (EVR7)
Subcellular Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, Cell junction, adherens junction, Cell junction, Cell membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cell junction, synapse
Protein Families Beta-catenin family
Tissue Specificity Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (Pub
Database Links

HGNC: 2514

OMIM: 114500

KEGG: hsa:1499

STRING: 9606.ENSP00000344456

UniGene: Hs.476018

Pathway Hippo signaling pathway
Melanogenesis
Wnt signaling pathway
Focal adhesion
Adherens junction
Signaling pathways regulating pluripotency of stem cells
Leukocyte transendothelial migration
Rap1 signaling pathway

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