Code | CSB-RA966627A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
CUSABIO immunized a rabbit with a human FGFR3-derived peptide, followed by the isolation of splenocytes from the immunized rabbit. RNA was extracted from the splenocytes and converted into cDNA through reverse transcription. The FGFR3 antibody genes were extended using degenerate primers. PCR amplification yielded fragments of the FGFR3 antibody gene, which were then purified and cloned into an expression vector. The recombinant expression vector was transfected into a host system for antibody production. The resulting FGFR3 recombinant monoclonal antibodies were purified using affinity chromatography from the cell culture supernatant. The binding specificity and affinity of the recombinant FGFR3 monoclonal antibody were confirmed through ELISA and WB applications. This antibody specifically targets the human FGFR3 protein.
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