Code | CSB-RA007479A1068phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
The phospho-EGFR (Y1068) recombinant monoclonal antibody is yielded through the implementation of protein technology and DNA recombinant techniques. Initially, animals are immunized with a synthesized peptide derived from human phospho-EGFR (Y1068). Next, B cells are obtained from the mice. Through a screening process, positive B cells are selected and undergo single clone identification. The light and heavy chains of the phospho-EGFR (Y1068) antibody are then amplified via PCR and inserted into a plasmid vector, creating a recombinant vector, which is transfected into host cells for the expression of the antibody. Subsequently, the phospho-EGFR (Y1068) recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It is recommended for use in ELISA and WB for the detection of human EGFR phosphorylated at Y1068 residue.
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