Code | CSB-E12124h |
Size | 96T,5×96T,10×96T |
Promotion | |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human EGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:5 | Average % | 101 | |||||
Range % | 95-104 | ||||||
1:10 | Average % | 99 | |||||
Range % | 91-103 | ||||||
1:20 | Average % | 94 | |||||
Range % | 87-98 | ||||||
1:40 | Average % | 108 | |||||
Range % | 97-112 |
The recovery of human EGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 103 | 96-107 | |||||
EDTA plasma (n=4) | 93 | 84-97 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
![]()
|
The product CSB-E12124h is a sandwich ELISA kit developed to measure concentrations of human EGFR in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of EGFR in samples. The color intensity is measured at 450 nm via a microplate reader.
EGFR regulates epithelial tissue development and homeostasis in a physiological context. It is involved in the growth, differentiation, maintenance, and repair of various tissues and organs also including the nervous system. In pathological settings, mostly in lung and breast cancer and in glioblastoma, EGFR drives tumorigenesis. EGFR is frequently mutated and/or overexpressed in different types of human cancers. It is a key factor in epithelial malignancies, and its activity increases tumor growth, invasion, and metastasis. Overexpression of EGFR and its ligand TGFα by many carcinomas correlates with the development of cancer metastasis, resistance to chemotherapy, and poor prognosis.
There are currently no reviews for this product.