EGFR

What is EGFR?

EGFR, also known as ErbB-1 or HER1, is an important transmembrane receptor of the epidermal growth factor receptors family. Besides EGFR, this family members also include HER2 (erbB2, NEU), HER3 (erbB3) and HER4 (erbB4). EGFR plays an important role in the physiological processes of cell growth, proliferation and differentiation. The abnormality of EGFR signal pathway is closely related to the occurrence of many diseases.

The Structure of EGFR

Human EGFR gene is located on chromosome 7p and encodes a single-chain transmembrane protein with a molecular weight of 134 kDa, which contains 1210 amino acid residues. The protein was further glycosylated at the 11th to 12th aspartic acid residues to form a mature EGFR, with a molecular weight of 170 kDa. EGFR is highly conserved in evolution, and the homology of EGFR between human and mouse is as high as 80%.

EGFR can be divided into three parts: extracellular region, transmembrane region and intracellular region.

The extracellular domain, the N-terminal of EGFR, contains 621 amino acid residues. This part contains the binding domain of the ligand EGF, which can mediate the binding of the ligand and EGFR.

The transmembrane region contains 23 amino acid residues, which is a hydrophobic region with helical structure.

The intracellular region consists of 542 amino acid residues, which can be divided into three parts: Proximal membrane region, tyrosine protein kinase region and C-terminal region.

The Expression of EGFR

EGFR is distributed on the surface of mammalian epithelial cells, fibroblasts, glial cells, keratinocytes and so on. EGFR is also expressed in a variety of human malignant tumor tissues and tumor cell lines.

Ligand of EGFR

EGFR ligand mainly include: Epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), betacellulin (BTC), heparin-binding EGF (HBEGF) and epiregulin (EPR). EGF and TGF- α are the two most important ligands of EGFR.

The Function of EGFR

EGFR is located on the cell surface. After being activated by ligand, EGFR is dimerized and induces intrinsic protein kinase activation, and further activates downstream cellular signal pathways: Ras/Raf/MEK/ERK/MAPK pathway, PI3K/Akt (PKB) pathway and JAK/STAT pathway. Signal transduction ultimately mediates a series of processes such as cell differentiation, survival, migration, invasion, adhesion and cell damage repair.

EGFR is often overexpressed and out of control in human tumors, which is closely related to tumor cell proliferation, angiogenesis, tumor invasion, metastasis and inhibition of apoptosis.

Popular Product

● Recombinant Human Epidermal growth factor receptor(EGFR),partial (CSB-MP007479HU)

Validated Data

High Purity Validated by
SDS-PAGE

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Cetuximab, the EC₅₀ of human EGFR protein is 0.6919-1.047 ng/ml.

Excellent Bioactivity Validated by LSPR Assay

Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9nM as detected by LSPR Assay.

● EGFR Recombinant Monoclonal Antibody CSB-RA159341MA1HU

Validated Data

IHC

IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

FC

Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA159341MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.