Human TNF-α ELISA Kit is designed to quantify human TNF-α levels in the cell culture supernates. It quantitates human TNF-α with 1.95 pg/mL sensitivity and shows excellent specificity for human TNF-α. This human TNF-α ELISA kit includes both the capture and the detection antibodies for TNF-α as well as avidin conjugated Horseradish Peroxidase (HRP) and TMB substrate solution, which are the reagents used in color development step. The produced color develops in proportion to the amount of TNF-α in the sample. The color development is stopped and the color intensity is measured using a microplate reader.This ELISA kit has been cited in 95 publications.
TNF-α is an inflammatory cytokine generated by macrophages/monocytes during acute inflammation. It contributes to a wide range of signaling events within cells, resulting in necrosis or apoptosis. It also plays an important role in the resistance to infection and malignancies. TNF-α binds to two different receptors TNFR1 and TNFR2, activating a downstream signal cascade, including NF-κB and MAPK pathways, evoking a variety of cellular responses, such as inflammation, host defense, cell survival, differentiation, and proliferation. Dysregulation of TNF-α signaling is related to chronic inflammation and autoimmune diseases. TNF-α inhibitors have been successfully produced and employed in the clinical treatment of autoimmune disorders such as Crohn's disease (CD) and RA due to TNF-α participation in the pathogenesis of autoimmune diseases.