Code | CSB-YP023955HU |
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Size | $276 |
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Introducing Recombinant Human TNF, a high-quality protein designed for use in immunology research. This partial-length protein encompasses the 77-233aa expression region of Tumor necrosis factor, also known as Cachectin or TNF-alpha. Expressed in yeast and equipped with an N-terminal 6xHis-tag for easy purification, this protein is perfect for researchers requiring a reliable and effective TNF source.
Our Recombinant Human TNF boasts a purity of greater than 90%, as confirmed by SDS-PAGE, providing assurance that the protein will deliver optimal results in your experiments. While endotoxin levels have not been tested, the activity of this protein has been measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL, indicating high biological potency.
Provided as a lyophilized powder, Recombinant Human TNF ensures convenient storage and use for a variety of experimental setups, making it an excellent choice for your research needs.
Applications : immunofluorescence staining
Review: Control NP and AF cells compared with those exposed for 12 hrs to TNF (25 ng/ml), TNF+CM, TNF+EVs or TNF+ SF. Localization of NF-κB p65 was visualized using confocal microscopy after immunofluorescence staining with NF-κB p65 antibody (green). Nuclei (white arrows) were stained with DAPI (blue).
By Anonymous
Applications : Thermos shift assay
Review: Thermal shift curves of unfolding transition of TNF-alpha in the presence of 100 pM, 10 pM, 1 pM, 0.1 pM 0.01 pM, and 0 pM of MY1. Data fit Boltzmann equation gave midpoint Tm of 81.13°C, 78.52°C, 77.07.5°C, respectively, (R2 > 0.9).
By Anonymous