TNF Antibody

Code CSB-PA07427A0Rb
Size US$166
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate
    All lanes: TNF antibody at 3.2µg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA07427A0Rb at dilution of 1:100

  • Immunofluorescence staining of HepG2 cells with CSB-PA07427A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TNF Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
APC1 antibody; APC1 protein antibody; Cachectin antibody; DIF antibody; Differentiation inducing factor antibody; Macrophage cytotoxic factor antibody; Tnf antibody; TNF superfamily member 2 antibody; TNF superfamily, member 2 antibody; TNF, macrophage derived antibody; TNF, monocyte derived antibody; TNF-a antibody; TNF-alpha antibody; TNFA antibody; TNFA_HUMAN antibody; TNFSF2 antibody; Tumor necrosis factor (TNF superfamily member 2) antibody; Tumor necrosis factor alpha antibody; Tumor necrosis factor antibody; Tumor necrosis factor ligand superfamily member 2 antibody; Tumor Necrosis Factor, Membrane Form antibody; Tumor necrosis factor, soluble form antibody
Raised in
Species Reactivity
Recombinant Human Tumor necrosis factor protein (77-233AA)
Immunogen Species
Homo sapiens (Human)

The TNF Antibody (Product code: CSB-PA07427A0Rb) is Non-conjugated. For TNF Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA07427B0Rb TNF Antibody, HRP conjugated ELISA
FITC CSB-PA07427C0Rb TNF Antibody, FITC conjugated
Biotin CSB-PA07427D0Rb TNF Antibody, Biotin conjugated ELISA
Purification Method
>95%, Protein G purified
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Tested Applications
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 3 reviews

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Applications : WB

Sample dilution: 1: 500

Review: The protein expression of TNF-α (0.53 ± 0.11 vs. 1.16 ± 0.19), FasL (0.31 ± 0.04 vs. 1.05 ± 0.17), and TRAIL (0.36 ± 0.07 vs. 0.97 ± 0.05) in HTR-8/SVneo cells were also decreased in the H-MEG3 group compared with the NC group. ***p < 0.001, **p < 0.01, *p < 0.05 by two-way ANOVA.

By Anonymous

Applications : Western blot analysis

Sample type: cell

Review: The expression of hub proteins (TNF, ESR1, MCL1, TBP, CD19, LCK, PCNA, CHEK1, and POLA1) in HeLa cells treated with N-CM and H-CM. The expression of hub proteins in HeLa cells treated with N-CM and H-CM for 24 h was analyzed with Western blotting.

By Anonymous

Applications : Immonoblot

Sample type: cells

Review: As far as Il-6 is concerned, its levels increased parallel to the increasing seawater temperature in both the gills and the mantle of Fthiotis and Lesvos individuals, with Fthiotis exhibiting statistically significant higher Il-6 levels in general compared to Lesvos.

By Anonymous

Target Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Gene References into Functions
  1. Genetic predisposition to rheumatoid arthritis in Russian population in the Republic of Karelia is associated with the presence of the GG TNF-alpha genotype. PMID: 30225702
  2. Treatment with 30 microg/ml curcumin also significantly diminishes the protein production of TNFalpha in Behcet's disease patients (p < .01) and healthy controls (p < .05) M1 macrophages. PMID: 29806793
  3. the protective role of the A allele in TNF-alpha 238A/G but not TNF-alpha 308A/G against the occurrence of juvenile idiopathic arthritis in the Caucasian population (Meta-Analysis) PMID: 30412082
  4. TNF-alpha expressed regionally in Epicardial Adipose Tissue may exert potent effects on the progression of coronary atherosclerosis PMID: 28931782
  5. The effect of the pro-inflammatory diet on concentrations of TNF-alpha was more pronounced in pregnant women reporting higher levels of stress. PMID: 30200631
  6. TNF-alpha -308G/A polymorphism is not associated with the risk to develop of bullous pemphigoid and alopecia areata in our Iranian cohort PMID: 29843231
  7. data support that only the promoter single-nucleotide polymorphism (SNP) rs1800629 within the TNF-alpha gene is associated with increased risk for developing Graves' disease (GD), especially in European population. Future large-scale studies are required to validate the associations between TNF-alpha gene and GD. PMID: 29440561
  8. We studied 173 polymorphisms to establish an association with the response to anti-TNF drugs in patients with moderate-to-severe plaque psoriasis (N=144). PMID: 27670765
  9. The SNP rs4819554 in the promoter region of IL17RA significantly influences the response to anti-TNF drugs at week 12. PMID: 27670766
  10. TNF-alpha promoter gene polymorphisms and/or haplotypes are risk factors of Nephrotic syndrome and resistance to steroid among Egyptian children PMID: 28803697
  11. The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for nasal polyposis in the north part of Iran and the minor frequency of G308A allele in the current study is slightly more than other major populations. PMID: 30003390
  12. results suggest, the interplay of pro-inflammatory cytokines IFN-gamma derived from CD4+T lymphocytes and TNF-alpha from CD14+ cells has no direct additive impact on parasite replication but induces IL-4 production. PMID: 29953494
  13. The single nucleotide polymorphisms rs361525, rs1800629, and rs17999645 of tumor necrosis factor-alpha were significantly correlated with the diagnosis of cervical cancer PMID: 29940817
  14. Rs11541076 in IRAK3, a negative regulator of TLR signalling, as a predictor of anti-TNF treatment response. PMID: 27698401
  15. In this Brazilian population, TNF and IL17 gene polymorphisms responsible for the expression of important inflammatory cytokines were associated with overall spondyloarthritis and, specifically, with ankylosing spondylitis and psoriatic arthritis, regardless of gender and HLA-B27 PMID: 29849482
  16. by restraining TNFR1 at the cell surface via sialylation, ST6Gal-I acts as a functional switch to divert signaling toward survival. These collective findings point to a novel glycosylation-dependent mechanism that regulates the cellular response to TNF and may promote cancer cell survival within TNF-rich tumor microenvironments. PMID: 29233887
  17. The TNF*A allele confer susceptibility to AIH in the Tunisian patients and is associated with increased production of TNF-alpha. Anti-TNF antibodies could be an alternative to the use of corticotherapy and may avoid the exacerbated immune response in Autoimmune hepatitis. PMID: 29845365
  18. Impairment in TNF, IL-1beta, and IL-17 production upon stimulation with mycobacterial antigens may contribute to the increased susceptibility to M. tuberculosis infection observed in HTLV-1 infected individuals. PMID: 29523325
  19. LL was significantly negatively correlated with PGC-1alpha, TNF-alpha, and IL-6 mRNA expressions. PGC-1alpha mRNA expression levels in paraspinal muscles may be affected by lumbar kyphosis. PMID: 30233161
  20. TNF-alpha-308G>A polymorphism affects the overall survival of cancer patients and is a potential therapeutic target for cancer. PMID: 30407345
  21. Many inflammatory pathologies are now recognized to be driven by aberrant TNF-induced cell death, which, in most circumstances, depends on the kinase Receptor-interacting serine/threonine-protein kinase 1 (RIPK1). [review] PMID: 29217118
  22. High TNF-alpha expression is associated with retinopathy of prematurity. PMID: 29274846
  23. Tumour necrosis factor-alpha selectively reduces BMPR-II transcription and mediates post-translational BMPR-II cleavage via the sheddases, ADAM10 and ADAM17 in pulmonary artery smooth muscle cells. PMID: 28084316
  24. Polymorphisms of IL-1betab and TNF-a are not a risk of ICC, but an individual with O. viverrini infection has an effect on all genotypes of the TNF-alpha gene that might promote intrahepatic cholangiocarcinoma . Primary prevention of intrahepatic cholangiocarcinoma in high-risk areas is based on efforts to reduce O. viverrini infection. PMID: 30139338
  25. In placenta, when gestational age was controlled for, partial correlation revealed a significant positive correlation between TNF-alpha and MMP-9 only in the second trimester. PMID: 28820024
  26. Study shows that in human endometrial stromal cells, high tumor necrosis factor levels negatively affect the insulin action through decreased adiponectin signaling and glucose transporter type 4 protein. This could explain the failures observed in endometrial function of obese women with polycystic ovary syndrome. PMID: 28946816
  27. Three single nucleotide polymorphisms (SNPs) within P2X4R and two SNPs within CAMKK2 influenced concentrations of TNFalpha in peripheral blood mononuclear cells, but these SNP did not associate with risk for HIV-associated sensory neuropathy in South Africans. PMID: 29428485
  28. Results indicated that the proinflammatory cytokine TNF-alpha impairs endothelial tight junctions and promotes monocyte-endothelial cell adhesion by upregulating beta-site amyloid precursor protein enzyme 1 expression through activating PKC signaling and sequentially cleaving alpha-2, 6-sialic acid transferase 1. PMID: 28091531
  29. These findings reveal that PrP enhances the responses to TNF-alpha, promoting proinflammatory cytokine production, which may contribute to inflammation and tumorigenesis. PMID: 28900035
  30. This study found that the protein and mRNA expression levels of the cytokines TNF-alpha is significantly increased. PMID: 28476335
  31. Taking together, these results suggest that Wnt/beta-catenin signal pathway activation-dependent up-regulation of syncytin-1 contributes to the pro-inflammatory factor TNF-alpha-enhanced fusion between oral squamous cell carcinoma cells and endothelial cells. PMID: 28112190
  32. Data suggest that, in children with pediatric obesity, lifestyle weight-loss intervention results in down-regulation of serum cardiotrophin-1 (CTF1), interleukin-6 (IL6), and tumor necrosis factor-alpha (TNFA); expression of CTF1, IL6, and TNFA is also down-regulated in peripheral blood mononuclear cells after improvement in adiposity, body mass index, and waist-hip ratio. PMID: 28749076
  33. TNFalpha differently regulated the levels of PPARalpha, LXRalpha, and LXRbeta binding to the apoA-I gene promoter in THP-1 cells. Obtained results suggest a novel tissue-specific mechanism of the TNFalpha-mediated regulation of apoA-I gene in monocytes and macrophages and show that endogenous ApoA-I might be positively regulated in macrophage during inflammation. PMID: 29442267
  34. this is the first evidence to suggest that TET2 mutations promote clonal dominance with aging by conferring TNFalpha resistance to sensitive bone marrow progenitors while also propagating such an inflammatory environment. PMID: 29195897
  35. Anti-rotavirus effect of TNF-alpha was achieved by NFkappaB-regulated genes via the activation of classical nuclear factor kappaB (NF-kappaB) signaling. PMID: 29859235
  36. Results revealed that heterozygous genotype GA of TNF-alpha-238 (rs 361525) SNP significantly increased the risk of adverse-outcome (mortality rate), regardless of organ dysfunction or severity of sepsis. PMID: 29978383
  37. in the patients with primary depression, depressive symptoms were associated with TNF-alpha PMID: 30148175
  38. Addition of TNFalpha to podocytes causes CD80 upregulation, actin reorganization and podocyte injury. PMID: 29022109
  39. The results of the study suggest that the levels of C-reactive protein and tumor necrosis factor-alpha are important diagnostic markers of inflammation in patients with chronic pancreatitis and type 2 diabetes mellitus. PMID: 30280549
  40. TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID: 29949812
  41. the results of the present study suggested that overexpression of KLF15 in Eahy926 cells exhibited a protective effect against TNFalpha induced dysfunction via activation of Nrf2 signaling and inhibition of nuclear factor kappaB signaling. PMID: 29956764
  42. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
  43. Elevated A20 promotes TNF-induced and RIPK1-dependent intestinal epithelial cell death PMID: 30209212
  44. TNF-alpha GG genotype at -238 and GG haplotype at positions -308 and -238 were associated with Kawasaki disease in an Iranian population. PMID: 27455075
  45. We have shown that a TNFalpha gene polymorphism, rs1800629, is highly significantly associated with postmenopausal osteoporosis and BMD in the female Han Chinese population PMID: 29481288
  46. allele -308 A TNF-alpha may have a role in progression of rheumatoid arthritis in a North Indian population PMID: 28748515
  47. ATF3 mediates the inhibitory action of TNF-alpha on osteoblast differentiation and that the TNF-alpha-activated JNK pathway is responsible for the induction of Atf3 expression. PMID: 29605296
  48. TNF-alpha, DKK1, and OPG have roles in pathogenesis of knee osteoarthritis PMID: 28676900
  49. Btk acts in the TLR7/8 pathway and mediates Ser-536 phosphorylation of p65 RelA and subsequent nuclear entry in primary human macrophages. PMID: 29567473
  50. Results indicated that prolonged tumor necrosis factor (TNFalpha) exposure could have detrimental consequences to endothelial cells by causing senescence and, therefore, chronically increased TNFalpha levels might possibly contribute to the pathology of chronic inflammatory diseases by driving premature endothelial senescence. PMID: 28045034

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Involvement in disease
Psoriatic arthritis (PSORAS)
Subcellular Location
Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Protein Families
Tumor necrosis factor family
Database Links

HGNC: 11892

OMIM: 191160

KEGG: hsa:7124

STRING: 9606.ENSP00000398698

UniGene: Hs.241570

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