Human plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Code CSB-E07946h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05121
Lead Time 3-5 working days
Abbreviation SERPINE1
Target Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Alias PAI, PAI-1, PAI1, PLANH1, plasminogen activator inhibitor-1|serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1,tPAI-1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.125 ng/mL-200 ng/mL
Sensitivity 2.201 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
HGNC 8583
RGD 3249
MGI 97608
Precision

 Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess. 

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of human PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

References
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Function Serine protease inhibitor. Inhibits TMPRSS7
Involvement in disease Plasminogen activator inhibitor-1 deficiency (PAI-1D)
Subcellular Location Secreted
Protein Families Serpin family
Tissue Specificity Expressed in endothelial cells (PubMed:2430793, PubMed:3097076). Found in plasma, platelets, and hepatoma and fibrosarcoma cells.
Database Links

HGNC: 8583

OMIM: 173360

KEGG: hsa:5054

STRING: 9606.ENSP00000223095

UniGene: Hs.414795

Pathway HIF-1 signaling pathway
Hippo signaling pathway
p53 signaling pathway
Cellular senescence
Complement and coagulation cascades
AGE-RAGE signaling pathway in diabetic complications
Apelin signaling pathway

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